Both tips and pipette should guarantee a good locking between tip and cone. Optimal fit and tightness will help you to keep the relative error as low as possible, especially by pipetting smallest sample volumes.
- Uniform tip seat across all channels (channel-homogeneity)
Evenly and uniformly inserted tips ensure pipetting exact same sample volumes. Pipette features like haptic feedback give you the security over a correct fit without “rocking & banging”.
Check your pipetting technique. Immerse as little as possible, but deeply enough to avoid uptake of air. See more on (https://handling-solutions.eppendorf.com/liquid-handling/pipetting-facts/small-volumes/detailview/news/how-to-pipette-small-volumes-with-handheld-manual-pipettes
- Stick to the 384-well “beast”
Use high-throughput sample processing to your advantage! By processing one 384-well plate instead of four 96-well plates you save valuable time whilst increasing your sample throughput at factor 4.
Appropriate equipment and a correct technique can help you to reduce time and effort keeping maximum precision without losing your mind!