epMotion® 5075t NGS Solution
epMotion 5075 with MultiCon
View from back through epMotion 5075, deck fully equipped, gripper, magnetic bead plate
View from back through epMotion 5075, deck fully equipped, 8-channel tool
Gripper wants to remove magnetic bead plate from magnet adapter of epMotion
NGS workflow with automated lib prep and lab person
topview 5075t, thermal mixing module, no accesories, empty deck
sideview 5075t with plate on thermal module
epMotion 5075
sideview 5075t, thermal module, no accessories, empty deck
Esteve, Barcelona, Spain
Institute for Pathology, TU Munich, Germany
Institute of Biotechnology, Prague, Czech
NGS Made Easy
Product Information
NGS Made Easy - The epMotion 5075 NGS solution combines automated liquid handling and unique software features with the accessories and high-quality consumables you need for reliable Next Generation Sequencing library preparation and maximum NGS kit flexibility. Next Generation Sequencing sample preparation is a labor-intensive process, which requires experience, precision and accuracy to generate high-quality NGS libraries. Automating Next Generation Sequencing library preparation eliminates the risk of pipetting errors, provides reproducible results and increases your productivity. A vast list of automated Next Generation Sequencing sample prep methods has been qualified by kit manufacturers and customers to deliver sequencing results that are comparable or better to those from manual preparation. In addition, an intuitive software interface allows for ease-of-use and rapid method design, while providing the needed flexibility to optimize even the most complex Next Generation Sequencing workflows.You will find additional download material at the bottom of this page
Sets (2)
Accessories (74)
epServices (17)
2 Sets
Catalog no.
5075000962
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Catalog no.
5075000963
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Product Information
Technical Data
Applications
Features
The epMotion 5075 NGS solution includes everything to start automated NGS library preparation. The basic system is an epMotion 5075t with an integrated Eppendorf ThermoMixer® to mix, heat or cool samples and reagents, and a thermal module on deck position C2 for additional temperature control. A gripper for labware transport and 2x single- and 2x 8-channel pipetting tools to cover a volume range from 1 to 300 µL. Next Generation Sequencing specific accessories (Thermoblock 96 OC, 2 x Thermoadapter 96-well, Rack ILMN tubes, ReservoirRack, Eppendorf Magnet Adapter FLX, waste bag holder) are included, plus NGS specific consumables (Eppendorf twin.tec® PCR Plate 96 semi-skirted, epT.I.P.S.® Motion pipette tips as reload system with filter PCR clean 50 µL & 300 µL plus boxes and clips, LiquidWasteTub 400 mL, 30 mL reservoirs, waste bags). The epMotion 5075 NGS solution liquid handling robot is available from stock with and without CleanCap option in all different voltage versions.
ThermoMixer®
Fully integrated ThermoMixer with 2DMix-Control improves yield and application results, e.g. resuspending bacteria pellets or magnetic beads. Peltier element for heating and cooling of sample and/or reagents. The following epMotion versions are equipped with ThermoMixer capability, M5073, 5075t, 5075m, 5075vt. A ThermoMixer upgrade product is available for epMotion 5075l, 5075v, and successor versions 5075LH and 5075VAC.
Sample preparation for Next Generation Sequencing (NGS)
involves extensive manual processing, is highly time-consuming and needs intense concentration. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results. The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of pipetting errors.
Gripper Tool
The gripper tool allows transport of labware on the worktable of epMotion 5073 or 5075 versions. It is loaded by automatic tool exchange and can be programmed easily in the software. The stacking of up to 5 plates is possible.
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| Specifications | epMotion® 5075t NGS solution | epMotion® 5075tc NGS solution |
|---|
| Application | NGS | NGS |
| Heating rate | 5 °C/min, on the block | 5 °C/min, on the block |
| Volume range | 0,2 µL – 1 mL | 0,2 µL – 1 mL |
| Power supply | 100 – 240 V ±10 %, 50 – 60 Hz ±5 % | 100 – 240 V ±10 %, 50 – 60 Hz ±5 % |
| Max. power consumption | 700 W | 700 W |
| Dimensions (W × D × H) | 107 × 61 × 77 cm / 43 × 24 × 30 in | 107 × 61 × 77 cm / 43 × 24 × 30 in |
| Weight w/o accessories | 97,1 kg / 213,9 lb | 103,1 kg / 220,6 lb |
| Pipetting type | Air cushion system | Air cushion system |
| Autoclavable | yes (tools) | yes (tools) |
| SLAS/ANSI positions | 14.5 | 14.5 |
| Gripper carrying capacity | 1,200 g | 1,200 g |
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Eppendorf ThermoMixer® |
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| Cooling rate | 3 °C/min, on the block, above RT | 3 °C/min, on the block, above RT |
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Dispensing tool1)2) |
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| Random measurement error (1 µL) | ≤3 % | ≤3 % |
| Systematic measurement error (1 µL) | ±5 % | ±5 % |
| Random measurement error (25 µL) | ≤0.6 % | ≤0.6 % |
| Systematic measurement error (25 µL) | ±1.5 % | ±1.5 % |
| Random measurement error (50 µL) | ≤0.4 % | ≤0.4 % |
| Systematic measurement error (50 µL) | ±1.2 % | ±1.2 % |
| Random measurement error (150 µL) | ≤0.4 % | ≤0.4 % |
| Systematic measurement error (150 µL) | ±1 % | ±1 % |
| Random measurement error (1,000 µL) | ≤0.15 % | ≤0.15 % |
| Systematic measurement error (1,000 µL) | ±0.7 % | ±0.7 % |
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1)in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °C 2)See Eppendorf Application Note 168 for typical pipetting performance |
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Thermal module |
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| Heating rate | 9 °C/min | 9 °C/min |
| Cooling rate | 4 °C/min | 4 °C/min |
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Detector |
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| Optical sensor | Contact-free recognition of fill level, inserted tools, labware, tip types and quantities | Contact-free recognition of fill level, inserted tools, labware, tip types and quantities |
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Conductor – X, Y, Z Positioning |
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| Random measurement error | ±0.1 mm | ±0.1 mm |
| Systematic measurement error | ±0.3 mm | ±0.3 mm |
- Like 5075l, plus
- Bead applications with mixing and tempering
- Sequencing and PCR clean-up
- Nucleic acid purification
- Cell disruption
- Immunoassays
- Next Generation Sequencing library preparation
- Same as 5075t, plus
- Industry-proven, large library of manufacturer and customer qualified NGS methods enables sequencing results comparable or better to those from manual preparation
- Intelligent Tool Selection automatically decides which tools (single or 8-channel) to use for fastest run time – ideal when sample numbers change frequently or are not a multiple-of-8
- Optimized bead clean-up on magnetic plate adapter to prevent carryover contamination
- Email notification option keeps you informed about the status of your library prep run
- Increased stacking capabilities for plates and tip boxes for more efficient use of deck space
- Integrated, high-performance ThermoMixer and thermal module allow for efficient mixing and reliable temperature control
- CleanCap option provides UV decontamination and HEPA air filter to protection samples from contaminations
- Automating Next Generation Sequencing library preparation using epMotion provides reproducible results due to its outstanding pipetting accuracy (0.31 % at 1 µL) and precision (1.97 % at 1 µL) and by eliminating the risk of pipetting errors
- Integrated LEDs for visual feedback of system status like idle, run in progress or run finished
Downloads: epMotion® 5075t NGS Solution
Webinar: Liquid Handling for NGS Workflows
Generating high-quality NGS libraries is a labor-intensive process that requires experience, precision, and accuracy. In this webinar, you will learn:
- The basics of NGS workflows & expert advice on getting started with NGS in your lab
- Practical tips and tricks for manual and automated liquid handling
- How Eppendorf epMotion can help you achieve reliable and reproducible sequencing results
Eppendorf SE
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