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Eppendorf BioSpectrometer® fluorescence
BioSpectrometer fluorescence front view
BioSpectrometer fluorescence product_002
BioSpectrometer fluorescence product_003
BioSpectrometer fluorescence with cuvette
BioSpectrometer fluorescence with cuvette
BioSpectrometer fluorescence
Eppendorf BioSpectrometer® fluorescence: Low volume of samples needed when Eppendorf µCuvette is used
µCuvette G1.0 closed
µCuvette G1.0 mechanism
UVette light paths
VIS Cuvette macro
VIS Cuvette semi-micro
Eppendorf BioSpectrometer fluorescence
University of East London
Product Information
Focusing on very small sample amounts?The integrated fluorescence unit of the BioSpectrometer fluorescence increases the measuring range by a factor of 1,000, for example, to detect DNA. This enables reliable quantification down to a concentration of 1.0 pg/µL. Measurements can be flexibly executed in the UV/Vis or fluorescence range by using this detection instrument.
You will find additional download material at the bottom of this page
Single Devices (1)
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Accessories (8)
epServices (10)
1 Single Devices
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Catalog no.
6137000006
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€ 6.975,00
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Product Information
Technical Data
Applications
Features
The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. UV/Vis assays, fluorescence assays and semi-micro/micro-volume measurements can all be carried out on a single instrument without the need for external control (PC/Laptop). Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. Pre-programmed (modified) methods for Qubit DNA quantification kits (SS, HS, BR) expand the flexibility and functionality of the instrument. Additionally, the use of the Eppendorf µCuvette G1.0 for fluorescence assays (5 µL loading volume) allows for a vast reduction in reagent usage.
Standard data
Standard values are to be defined and measured by the instrument.
Set the standard curve
Based on the standard data, the instrument calculates the standard curve. This includes the linear regression value as well as the coefficient of determination.
Sample results
The measured sample value is recalculated based on the standard curve. Finally, you get a concentration value for your sample.
Annoyed by uncomfortable handling?
Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
• Guided software for easy handling
• Clear indications at cuvette shaft for convenient handling
• Direct keys for convenient handling
• Small footprint to enable position in direct arm reach at your bench
• Standardized »user-interface concept« for intuitive handling of devices
Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/
Struggling with paper-based documentation?
The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.
Start a free 30-day trial!
Visit www.eLABJournal.com
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| Specifications | Eppendorf BioSpectrometer® fluorescence |
|---|
| Interfaces |
• USB master: for USB stick and thermal printer DPU-S445 • USB slave for connecting to a PC (All functions available without PC) • Serial interface RS-232: for thermal printer DPU-414 • Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument |
| Power supply | 100 – 240 V ±10 %, 50 – 60 Hz |
| Dimensions (W × D × H) | 29,5 × 40,0 × 15,0 cm / 11,6 × 15,7 × 6 in |
| Weight w/o accessories | 5,4 kg / 12 lb |
| Random error wavelength | ≤0.5 nm |
| dsDNA concentration range (for 1 to 10 mm path length) | 2.5 ng/µL – 45,450 ng/µL (with fluorescence down to 1 pg/µL) |
| BSA concentration range (UV 280 nm) µCuvette® G1.0 | 758 ng/µL – 45,450 ng/µL |
| dsDNA concentration range (UV 260 nm) µCuvette® G1.0 | 25 ng/µL – 1,500 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette® 2 mm | 12.5 ng/µL – 750 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette® 10 mm | 2.5 ng/µL – 150 ng/µL |
| BSA concentration range (UV 280 nm) UVette® 2 mm | 379 ng/µL – 22,725 ng/µL |
| BSA concentration range (UV 280 nm) UVette® 10 mm | 76 ng/µL – 4,545 ng/µL |
| BSA concentration range (for 1 to 10 mm path length, calculation factor for BSA = 1.515 µg/µL) |
76 ng/µL – 45,450 ng/µL (with fluorescence down to 0.01 ng/µL) |
| Wavelength range absorption | Scan (nm): 200 – 830 at 1 nm increments |
| Wavelength Selection Increment | 1 nm |
| Scanning | yes |
| Wavelength range | 200 nm – 830 nm |
| Sample light path length (mm) | 8.5 mm |
| Measuring principle absorption | single-beam absorption spectrophotometer with reference beam |
| Measuring principle fluorescence | confocal filter fluorometer with reference beam |
| Light source absorption | Xenon flash lamp |
| Light source fluorescence | LED |
| Excitation wavelength fluorescence | 470 nm, bandwidth: 25 nm |
| Emission wavelength I fluorescence | 520 nm, bandwidth: 15 nm |
| Emission wavelength II fluorescence | 560 nm, bandwidth: 40 nm |
| Spectral bandwidth (mm) | ≤4 nm |
| Smallest step size | 1 nm |
| Absorption measuring range | 0 A – 3.0 A (260 nm) |
| Random error absorption |
≤0.002 at A = 0 ≤0.005 (0.5 %) at A = 1 |
| Systematic error wavelength | ±1 nm |
| Cuvette shaft | 12.5 mm × 12.5 mm |
| Detector Type | CMOS photodiode array, 1024 pixel |
| Power consumption |
Approx. 15 W during operating step Approx. 5 W with dimmed display |
| Mode absorption | Spectrophotometer |
| Mode fluorescence | Fluorometer |
| Fluorescence measuring range | 0.5 nM – 2,000 nM fluorescein (emission wavelength 520 nm) |
| Random error fluorescence | ±2 % at 1 nM fluorescein |
| Beam receiver fluorescence | Photodiodes |
| Operator guidance language | Spanish, Italian, French, English, German, Japanese |
| Method memory | >100 method programs |
- Ideal for NGS applications (micro-volume UV/Vis and fluorescence assays in a single instrument)
- dsDNA quantification down to 1 pg/µL with common kits, e.g., Pico Green®
- ssDNA quantification down to 10 pg/µL
- RNA quantification down to 20 pg/µL
- Oligo quantification down to 10 pg/µL
- Protein (BSA) quantification down to 0.01 ng/µL
- Quantification of GFP-binding proteins
- Growth measurement of GFP-producing bacteria
- All Eppendorf BioSpectrometer basic options
- Preprogrammed and freely programmable fluorescence methods
- Preprogrammed (modified) methods for Qubit DNA quantification kits (SS, HS, BR)
- Fluorescence measurement concentration of dsDNA down to 1 pg/µL, using Pico Green®
- Fluorescence measurement concentration of BSA down to 0.01 ng/µL
- Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
- Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein
- Single consumable (UVette) for UV/Vis and fluorescence assays
- Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
- Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email
Downloads: Eppendorf BioSpectrometer® fluorescence
Eppendorf SE
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