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96-Wells at Once – Faster and More Reproducible Cell Assays
Lab Academy
- Liquid Handling Workstations
- Efficiency
- Reproducibility
- Automation
- Essay
Cell Based Assays are an established standard tool in life science and performed in 96 and 384-well plates. They require multichannel pipetting to ensure quick, efficient, and reproducible handling – especially when multiple plates need to be processed. A manual 12-channel pipette, an electronic 12-channel pipette and a semi-automated 96-channel electronic pipette (epMotion 96) were compared for cell seeding, cytotoxic agent and assay reagent addition.
Cell viability was evaluated after treatment with a staurosporine dose-response curve. Profiles generated were comparable for each instrument used for cell seeding and reagent adding. Nevertheless, with a manual pipetting system, the assay reproducibility was affected as indicated by higher standard deviations.
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![](/media/MAIN/01-Lab-Academy/csm_Figure2_AN367_epMotion_b5103c32aa_700.jpg)
S/B and Z´factor calculated for CellTubes - Blue Cell Viability Assay according to the instrument used for cell seeding and reagent adding
![](/media/MAIN/01-Lab-Academy/csm_Figure4_AN367_manual_pipettes_b2b7d8d1fa_700.jpg)
Intra- and inter-plate CVs calculated for CellTubes- Blue Cell Visability Assay for the respective instruments used
With all assay performance parameters studied (signal-to-background ratio, Z’-factor, intra-plate and inter-plate CV), it was demonstrated that the assay robustness and reproducibility were significantly better when an electronic pipette (epMotion 96 or electronic pipette) was used.
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![](/media/MAIN/01-Lab-Academy/csm_Fig.5_tif_AN367_bbc12c4dae_700.jpg)
Among all instruments tested, the epMotion 96 appears as the fastest option since a complete cell-based assay requires only 4 minutes, that means 5 times less hands-on time than what is needed with the manual pipette.
- to read the whole Application Note.
- to read the whole Application Note.
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