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Stem Cell Bioprocessing

Stem cell expansion at large scale

Stem cell-based technologies are one of the most promising approaches to further advance modern medicine, drug discovery, and the development of novel food. Research and development in these application areas require large numbers of high-quality cells. Stirred-tank bioreactors facilitate efficient cell expansion when conventional 2D-culture systems reach their limits.

Stem cell production for applied research

How can stem cells be produced efficiently and reproducibly to suit the demands of applied research? Dr. Marcia Mata (Cell and Gene Therapy Catapult), Prof. Bernd Giebel (University Hospital Essen), and Dr. Philipp Nold (Eppendorf SE) share their knowledge on current trends and their thoughts on future developments in the field of stem cell research and applications.

Customer success stories: Expansion and differentiation of hiPSCs in bioreactors

Optimizing the cultivation conditions is crucial for the use of hiPSCs in applied research. Find some examples, of how our customers successfully use stirred-tank bioreactors for large-scale hiPSC expansion and differentiation as 3D cell aggregates.

Bioreactor development for your success

Stem cell culture is challenging. That is why within the EU Research and Innovation program, Horizon 2020, and in cooperation with its customers, Eppendorf further developed its bioreactors to suit the special needs of stem cells.

Discover the BioBLU® 0.3sc Single-Use Bioreactor optimized for slow stirring and cell aggregate formation.

  • The 8-blade impeller type ensures gentle mixing of your cell culture
  • Reduced cell settling and very good mixing already at low agitation speed reduces the stress to your stem cell culture
  • Improved DO-cap with chamfer corners and reduced overall diameter reduces cell sedimentation on the cap
  • Reusable glass bioreactor versions are also available
  • Compatible with the DASbox® Mini Bioreactor System

Our 8-blade impeller supported 3D cell aggregate formation in a DASbox® Mini Bioreactor and efficiently kept the aggregates in suspension. Read more about how this impeller was used for the cultivation of 3D cell aggregates at the Instituto de Biologia Experimental e Tecnológica (iBET) in Portugal

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Our bioprocess specialists are happy to provide further support. Please contact us to discuss the needs of your individual application.
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Production of stem cell-derived exosomes

Stem cell-derived exosomes are currently of great interest as cell-free therapeutic tools due to their strong diagnostic and therapeutical potential in various diseases models. To explore the use of exosomes in preclinical research, large amounts need to be produced.
  • Download expert interview: Get to know more about the challenges and trends in stem cell-derived extracellular vesicle bioprocessing
  • Watch webinar to learn more about the benefits of stirred-tank bioreactors for exosome production
  • Download application note about exosome production at small scale, using the DASbox® Mini Bioreactor System
  • Download application note about exosome production at bench scale, using the SciVario® twin bioreactor control system

Expanding mesenchymal stem cells in bioreactors

Mesenchymal stem cells (MSCs) are multipotent cells derived from the bone marrow, from adipose tissue, cord blood or other tissues related to the embryonic mesoderm. Read, how they can be cultivated on microcarriers in stirred-tank bioreactors, while conserving their potential to differentiate into use cell like osteocytes or chondrocytes.

Growing meat in bioreactors

Can bioreactors revolutionize the way we produce animal products, such as meat and leather? Can we make meat more sustainable and increase animal welfare? Read expert opinions about the use of stem cells for cultured meat

Join the stem cell community!

Interview with Dr. Philipp Nold

Dr. Nold joined the Eppendorf Bioprocess Center as an Infield Application Specialist for stem cells. In this interview he speaks about the latest advances in bioprocessing.

Main aspect of this interview is: Biopharma know-how meets bioreactor innovation in upstream bioprocessing.

Click here to read the interview

Induced pluripotent stem cells

Since the pioneering development of cell reprogramming by Shinya Yamanaka in 2006, induced pluripotent stem cells (iPSCs) have risen to be the most promising alternative to ethically problematic embryonic stem cells. Controlled cultivation and scale-up in clinical grade as well as ways to control their differentiation towards their final destiny will be key steps towards commercial use of these cells.

Cultivation of cell aggregates

For many applications like disease modeling, drug toxicity assessment, and manufacture of stem cell-based products, three-dimensional (3D) cell aggregates are of great interest. Researchers at the Instituto de Biologia Experimental e Tecnológica (iBET) in Portugal have tested the suitability of the Eppendorf DASbox® Mini Bioreactor System for the cultivation of the human tumor cell line H157 as 3D cell aggregates.

Dr. Robert Zweigerdt and his team at Hannover Medical School are investigating human pluripotent stem cell culture for clinical use. They established a process using the Eppendorf DASbox® Mini Bioreactor System equipped with BioBLU® Single-Use Vessels. As part of the EU-funded research project TECHNOBEAT , Eppendorf and the Hannover Medical School aim at scaling up the culture and develop a GMP-compliant process.

Researchers from TissUse® GmbH generated hiPSC-derived neurospheres for drug research applications. They demonstrated the potential of the DASbox Mini Bioreactor for such a process.

Stem Cell Community Day

The Stem Cell Community Day connects researchers from all over the world to review recent advances in the area of stem cells, with a special focus on cultivation in stirred-tank bioreactors.

eBook: Advancing medicine - Can cell and gene therapies paint a picture of perfect future health?
(The Medicine Maker, sponsored by Eppendorf)
3.2 MB, PDF

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At our 1st Stem Cell Community Day in April 2017 we brought together experts from industry and academia, to discuss latest achievements, challenges, and chances in stem cell bioprocessing for research and commercial manufacturing.

A selection of our customers