DASbox® Mini Bioreactor System
Product Information
With small working volumes, compact design and advanced control and information management options the Eppendorf DASbox Mini Bioreactor System addresses demands in research and early process development. Combined with the DASware Software Suite it is an optimum tool for Design of Experiments (DoE) approaches as well as standardising and streamlining process development.You will find additional download material at the bottom of this page
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Product Information
Technical Data
Applications
Features
Product Information
In addition to using industry standard glass bioreactors, the DASbox can be equipped with Eppendorf BioBLU 0.3 Single-Use Bioreactors, all fully instrumented single-use mini bioreactors.
Technical Data
Specifications | DASbox® Cell Culture | DASbox® Cell Culture Single-Use | DASbox® Microbiology | DASbox® Microbiology Single-Use |
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Application | Cell culture | Cell culture | Microbiology | Microbiology |
Number of parallel units | Up to 24 | Up to 24 | Up to 24 | Up to 24 |
Software | DASware control, other DASware optional | DASware control, other DASware optional | DASware control, other DASware optional | DASware control, other DASware optional |
User Interface | Process computer with monitor | Process computer with monitor | Process computer with monitor | Process computer with monitor |
Typical power consumption (4-fold system without process computer) |
168 W (at 230 V)/ 154 W (at 115 V) |
168 W (at 230 V)/ 154 W (at 115 V) |
168 W (at 230 V)/ 154 W (at 115 V) |
168 W (at 230 V)/ 154 W (at 115 V) |
Dimensions (W × D × H, 4-fold system without process computer) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
Product weight | 44 kg | 44 kg | 44 kg | 44 kg |
Typical weight | 44 kg | 44 kg | 44 kg | 44 kg |
Bacteria/Yeast/Fungi | yes | yes | yes | yes |
Mammalian/Animal | yes | yes | yes | yes |
Insect | yes | yes | yes | yes |
Interchangeable Vessels | yes | yes | yes | yes |
Validatable | no | no | no | no |
Stem cells | yes | yes | yes | yes |
Bioreactors |
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Vessels | Glass vessels | Single-use vessels | Glass vessels | Single-use vessels |
Sterilization | Autoclavable | Pre-sterilized | Autoclavable | Pre-sterilized |
Total volume | 350 mL | 380 mL | 350 mL | 380 mL |
Agitation |
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Drive | Direct overhead drives | Magnetic overhead drives | Direct overhead drives | Magnetic overhead drives |
Speed ranges | 20 – 2500 rpm | 20 – 500 rpm | 20 – 2500 rpm | 20 – 2000 rpm |
Impellers | Marine | Pitched-blade | Rushton-type | Rushton-type |
Gassing |
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Gas supply | TMFC; overlay and/or sparger | TMFC; overlay and/or sparger | TMFC; sparger | TMFC; sparger |
Standard gas flow rates | 0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO2 | 0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO2 | 0.2 – 25 sL/h, 0.2 – 18 sL/h CO2 | 0.2 – 25 sL/h, 0.2 – 18 sL/h CO2 |
Standard gas mixing | Air, N2, O2 and/or CO2 | Air, N2, O2 and/or CO2 | Air, N2, O2 and/or CO2 | Air, N2, O2 and/or CO2 |
Feeding |
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Feed lines per vessel |
2 (standard)/ 4 (optional) |
2 (standard)/ 4 (optional) |
2 (standard)/ 4 (optional) |
2 (standard)/ 4 (optional) |
Standard feed rates (depending on tube diameter) | 0.3 – 9.5 mL/h to 13 – 420 mL/h | 0.3 – 9.5 mL/h to 13 – 420 mL/h | 0.3 – 9.5 mL/h to 13 – 420 mL/h | 0.3 – 9.5 mL/h to 13 – 420 mL/h |
Monitoring and Control |
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Temperature control | Liquid-free heating and cooling (Peltier) | Liquid-free heating and cooling (Peltier) | Liquid-free heating and cooling (Peltier) | Liquid-free heating and cooling (Peltier) |
Standard temperature range (at 25° C room temp.) | 10 – 60°C | 10 – 45°C | 10 – 60°C | 10 – 45°C |
pH control | CO2/base, and other set-ups | CO2/base, and other set-ups | Acid and/or base, and other set-ups | Acid and/or base, and other set-ups |
DO control | Cascade (O2 concentration, gas flow rate), and other set-ups | Cascade (O2 concentration, gas flow rate), and other set-ups | Cascade (agitation speed, O2 concentration, gas flow rate), and other set-ups | Cascade (agitation speed, O2 concentration, gas flow rate), and other set-ups |
ORP (redox) measurement | - | - | Optional (select redox or level) | Optional (select redox or level) |
Level/foam | Optional | Optional | Optional (select redox or level) | Optional (select redox or level) |
OD measurement |
Optional (DASGIP OD4) |
Optional (DASGIP OD4) |
Optional (DASGIP OD4) |
Optional (DASGIP OD4) |
Exhaust condensation | Liquid-free (Peltier) | Liquid-free (Peltier) | Liquid-free (Peltier) | Liquid-free (Peltier) |
Exhaust analysis | - | - |
Optional (DASGIP GA4) |
Optional (DASGIP GA4) |
Applications
- Process development in cell culture and microbiology
- Controlled cultivation of stem cells
- Design of Experiments (DoE)
- Media optimization
- Clone and cell line screening, strain characterization
Features
- Parallel set-up of up to 24 bioreactors
- Excellent scalability and reproducibility in both microbial and cell culture applications
- Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as BioBLU 0.3 Single-Use Bioreactors
- Small working volumes save on the amount of cell material, medium and supplements required
- Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel
- Individual temperature control with liquid-free heating and cooling (Peltier)
- Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode
- LC display with key process parameters and integrated alarm function simplifies monitoring
- Fully mass flow-controlled gas mixing with individual gas mixture from air, N2, O2, and CO2, each directable either to headspace or sparger
- Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available
- Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h
- Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable
- Optional pull-out system for enhanced accessibility of bioreactors and control unit