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GMP info

Regarding suitability of Eppendorf bioprocess equipment in GMP-regulated applications, please reach out to your Eppendorf sales representative.

Monoclonal Antibody Production

Monoclonal antibody production using scalable bioprocess systems
Monoclonal antibody-based immunotherapy is a dominant and growing industry producing a promising class of therapeutics such as targeted anticancer drugs. In order to stay competitive, it’s essential that production can be made more efficient and cost-effective whilst still improving upon quality and concentration. Here you can find out how Eppendorf can help you to optimize your bioprocess for monoclonal antibody (mAb) production.
Monoclonal antibodies are genetically engineered molecules that are designed to act as substitute antibodies and restore, improve, alter, or mimic the immune system's defense against undesirable cells, such as cancer cells. Originally, for monoclonal antibody production mAb-producing cells (hybridomas) were generated by fusing immortal myeloma cells with antibody-producing B cells. Today, Chinese Hamster Ovary (CHO) cells are often used as an expression system. Antibody production in bacteria or yeast offers greater speed, however there are challenges associated with glycosylation. Find out more about antibody production in our infographic.

mAB discovery workflow

Antibody development is not without its challenges. Research teams are under increasing pressure to produce specific, high-quality therapeutic treatments within ever-tighter time and budget restrictions. As such, it is essential to optimize each step of the monoclonal antibody production process, from transfection, to clone selection, to process control and scale-up. From choosing candidate cell lines to scale-up, Eppendorf can help you to maintain a competitive edge by providing the tools, expertise, and knowledge needed to improve and regulate antibody yield and quality.

mAB solutions for biopharma
mAB discovery workflow

Expert knowledge: Optimize your bioprocess

Parallel, small-scale bioreactor system, which can help accelerating bioprocess development

Accelerate process development

Learn how the right equipment can accelerate your antibody process development through optimized, real-time parameter control, the ability to set up parallel and scalable reactions, and improved efficiency. With lower development costs and a shorter time for the product to reach the market, your business can stay competitive in an increasingly demanding market.

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Bioreactor control system, which can help in bioprocess optimization

Improve reproducibility and yield

Antibody production relies upon the interplay of several bioprocess parameters to influence yield and quality. Bioprocess optimization can help to improve reproducibility, reduce batch-to-batch variation, and increase yields and product quality, helping to save time and money in antibody production. This is especially important when seeking to scale up or perform a process validation. Here, we will consider the factors that can contribute to inconsistent results and offer strategies to increase the reproducibility and productivity of cell culture bioprocesses for the production of antibodies.

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Choose the right bioreactor system for monoclonal antibody production

Get to know more about cell culture in bioreactors and learn more about the Eppendorf solutions for upstream bioprocessing.

Parallel, small-scale bioprocess systems‌

Parallel small-scale bioreactor systems like the DASbox® Mini Bioreactor System support customers in implementing QbD principles in their development phase. These high-throughput bioreactor systems are designed for parallel cultivations, making them ideal for experimental design, parallel comparison studies and downscaling experiments.

Parallel small-scale bioreactor system, which supports users in implementing QbD principles

Single-use bioreactors‌

From small scale to pilot production scale‌

Software‌

How our customers use our products

Find some examples of how our customers use Eppendorf bioprocess equipment for monoclonal antibody production.

UGA Biopharma: Scaling-up from small to bench scale

Scaling the process up to larger volumes is a critical milestone in the development of monoclonal antibodies. Read, how scientists at UGA Biopharma scaled-up a biosimilar production process from small to bench scale using the kLa value as a scale-up criterium.

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KTH Royal Institute of Technology: Small-scale perfusion

Small scale perfusion systems are desired to save resources and reduce costs during process development, however, they are not very well established. Read how researchers at the KTH Royal Institute of Technology in Sweden established ATF perfusion cultivation of an antibody-producing CHO cell line at a working volume of only 230 mL.

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GenMab A/S: Antibody development

Scientists from GenMab A/S, an international biotechnology company specializing in the development of antibody-based therapeutics, were among the first SciVario® twin users. Get to know, which system’s features they find helpful to tackle challenges in antibody development.

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Alvotech®: Bioprocess development for biosimilar productiion

Bioprocess development for biosimilar production: Scientists from Alvotech® share insights on which process parameters are key to ensure consistent product quality and how automation can make the scientists’ lives easier.

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Roche Pharma Technical Development: Improve pH sensor recalibration

The pH of the culture medium strongly influences process performance. Read, how scientists at Roche Pharma Technical Development used exhaust CO2 readings for improved pH sensor recalibration to match the starting pH of carbonate-buffered systems – for increased process reproducibility cross plants, sites, and scales.

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APC: Raman analyzer integration

Scientists at APC, Ltd integrated a Raman analyzer and a model predictive controller algorithm with a DASGIP® Parallel Bioreactor System. Read, how this allowed them increasing the cell density of their CHO cell bioprocess by automizing glucose control.

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