Primary samples can be swabs, blood or other body fluids.
Storage and Management
- Samples can be stored in high quality Cryo tubes
- An Ultra-Low Temperature Freezer allows long term storage of primary samples
- The eLabJournal® and eLABInventory allow for effortless archiving and documentation
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To process the collected samples, samples need to be transferred to tubes or plates.
Manual Liquid Handling
- Mechanical or Electronic Single Channel Pipettes are ideal for precise reformatting of samples from primary vessels
- Filter Tips assure the sample integrity of primary samples
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Automated Liquid Handling
- Benchtop Pipetting Robots support you to deal with an increased sample throughput
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Plates and Tubes
- Leachable free plates and tubes for optimal processing and storage of aliquoted samples
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Storage and Management
- Samples can be stored using high-quality Cryo tubes
- Ultra-Low Temp Freezer allows long term storage of aliquoted samples
- eLabJournal® and eLABInventory allow painless archiving and documentation
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Nucleic Acid Extraction (also known as Nucleic Acid Purification) refers to the isolation of viral DNA or RNA used with magnetic-bead or filter/silica-based protocols.
Manual Liquid Handling
- Single Channel Pipettes are ideal for processing in tube format. Multichannel pipettes are optimal for processing in plate format.
- Filter Tips assure the sample integrity when handling primary samples
- A Dispenser and Combitips® help add reagents in a quick and precise way. They also help minimize handling steps, used materials and reduce the risk of cross-contamination.
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Automated Liquid Handling
- Benchtop Pipetting Robots allow walk-away automation of Magnetic-Bead-Based and Filter-Based Extraction Kits
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Plates and Tubes
- Leachable free plates and tubes for optimal results
- A wide range of consumables allow you to pick the best-suited consumable for your protocol, ranging from single tubes to deep well plates
- Safe and fast processing: easy one-hand handling, perfect fit on magnets, centrifugation and heat safe, automation-friendly
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Tempering and Mixing
- ThermoMixers are especially helpful for lysis steps, but also allow to help with other incubation steps, like resuspension and neutralization
- Suited for magnetic bead homogenization
- Easily fits the biosafety cabinet
- Interchangeable blocks make it easy to process and clean plates and tubes
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Centrifugation
- Key for processing filter-based protocols, especially single spin columns
- A wide variety of centrifuges and adapters allow you to handle plates and single vessels of each size and format
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Storage and Management
- Extracted nucleic acids can be stored in an Ultra-Low Temperature Freezer
- eLabJournal® and eLABInventory allow painless archiving and documentation
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Viral genomic material needs to be processed to generate libraries ready for Next-Generation sequencing. These libraries are mostly generated with commercially available reagent kits. The kit protocols involve a multi-step process that includes several magnetic bead cleanup steps, as well as multiplex PCR.
Manual Liquid Handling
- Single Channel Pipettes are ideal for processing samples in tube format. Multichannel Pipettes are optimal for processing in plate format
- Superior precision for highest reproducibility and handling low volumes
- A perfectly fitted tip for your pipette helps you to maximize precision
- Filter Tips drastically reduce the risk of cross-contamination
- A Dispenser and Combitips® help add reagents in a quick and precise way. They also help minimize handling steps, used materials and reduce the risk of cross-contamination.
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Automated Liquid Handling
- Benchtop pipetting robots allow the automation of whole NGS library preparation protocols or single critical steps
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Plates and Tubes
- Leachable free plates and tubes avoid interference with the NGS sequencing
- Semi skirted twin.tec® plates are ideally suited for NGS library preparation; as the combine up to 300 uL volume, a thermo-stable frame and perfect fit with 96-well magnets
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Tempering and Mixing
- ThermoMixers are important for all heat incubation steps
- Suited for magnetic bead homogenization
- Interchangeable blocks make it easy to process both plates and tubes
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Amplification
- Mastercycler® X50 offers high cycling speed and outstanding block homogeneity
- Ideally suited for demanding PCR applications like Multiplex PCR and critical heat incubation steps
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Centrifugation
- Crucial to avoid air bubbles that interfere with the reactions, and to spin down samples and reagents
- A wide variety of centrifuges and adapters allow you to handle plates and single vessels of each size and format
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Before the actual sequencing can take place, it is recommended that the quality and concentration of the libraries are checked. Some protocols also require a concentration normalization of the libraries. Pooling is usually the last step before the libraries are sequenced.
Manual Liquid Handling
- Single Channel Pipettes are ideal for precise qPCR set-up for QC, library distribution for normalization, and pooling before sequencing.
- Superior precision for highest reproducibility and handling low volumes
- A perfectly fitted tip for your pipette helps you to maximize precision
- Filter Tips drastically reduce the risk of cross-contamination
- A Dispenser and Combitips help add reagents and diluent in a fast and precise way, which allows you to speed up both QC and normalization
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Automated Liquid Handling
- Benchtop Pipetting Robots can support library QC and take over tedious normalization routines
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Plates and Tubes
- Leachable free plates and tubes reduce interference with the fluorescent signal of qPCR reaction for QC
- White plates help achieve maximum signal strength
- The ultra-clear film is perfect to seal plates
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Centrifugation
- Crucial to avoid air bubbles that interfere with the fluorescent signal, and to spin down samples and reagents
- A wide variety of centrifuges and adapters allow you to work with plates and single vessels of any size and/or format
- Concentrators can deal with low library concentrations
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Storage and Management
- Libraries can be stored in an Ultra-Low Temperature Freezer if they are not sequenced directly
- eLabJournal® and eLABInventory allow painless archiving and documentation
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The actual NGS run can detect and quantify viruses, viral subspecies, or viral genes. For further analysis, samples can be stored after sequencing for follow-up experiments.
Manual Liquid Handling
- Single Channel Pipettes are ideal to securely load pooled libraries to the flow cells.
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Storage and Management
- Plates and tubes can be stored using Ultra-Low Temperature Freezer
- The eLabJournal® and eLABInventory allow for effortless archiving and documentation
More about Sample storage solutions