Cell Biology

From Harvesting to Cell Manipulation

Cell Biology is a branch of biology that studies cells - the organelles they contain, their function, their physiological properties, their life cycle, the interactions with their environment etc. Cell Biology basic research can be divided into several subfields e.g. the study of cell metabolism, the study of cell genetics and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell communication and signaling. Research in cell biology overlaps to a great extent with other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology.



Harvesting & Preparation

Harvesting & Preparation

The speed adjustment of our electronic pipettes helps to remove the cells very carefully and precise. A variety of different consumables such as the well-known Safe-Lock Tubes or our Conical Tubes (15 mL and 50 mL) can be used for the preparation and/ or sample collection.

  • Centrifuges
    • Swing-bucket rotors and adapters accommodate tubes and bottles from 0.2 mL to 750 mL
    • Fixed-angle rotors for high-speed molecular biology applications
    • High centrifugation speed of up to 22,132 × g (14,000 rpm)
    • Centrifuge lid with soft-touch lid closure
    • Quiet operation to improve your work environment
    • Compact footprint saves valuable bench space

       

  • Biopur® consumables
    • Safety guaranteed, even for the most sophisticated applications
    • Reduced costs - inconclusive, usually expensive tests no longer necessary
    • "Ready for use" products reduce labor and time required
    • Individually blistered consumables and “micro batches” in re-sealable boxes provide effective protection against soiling or contamination
  • Tubes & plates
    • Hinged Eppendorf Safe-Lock lid prevents unintentional lid opening during incubation and storage
    • Polypropylene provides high resistance to chemicals, mechanical stress and temperature extremes
    • Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio-assay results
    • g-Safe®: Exceptional centrifugation stability up to 30,000 x g prevents sample loss due to tube breakage and provides extra safety when working with hazardous samples
    • Precise lid sealing for minimal evaporation rates during long-term storage
  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity.
Cell seeding

Cell seeding

The Easypet 3 in combination with the serological pipettes enables you to control the forces that act on your cells during pipetting. Besides this, Eppendorf offers a new generation of Cell Culture Consumables with improved handling and stacking performance, optimized cell accessibility and safety.

  • Pipette controllers
    • Intuitive and convenient speed adjustment made simple with the tips
      of your fingers
    • Lightweight, well-balanced and ergonomic design that allows for fatigue-free pipetting
    • Vibrant backlit LEDs provide optical feedback of the remaining battery life
    • Lithium polymer rechargeable battery offers long cordless runtime
    • Smooth setting of pump speed
  • Serological Pipettes
    • Individually wrapped
    • Easy to open paper/plastic wrap
    • Subunits with dispenser option to keep stored products safe
    • Harmonized and well-fitting operation with Easypet 3, compatibility
      with all existing pipet helper
    • Sharp and clear graduation for facilitated read-out
  • Automation
    • 4 ANSI/SBS worktable and unlimited virtual positions
    • Maximum pipetting accuracy from 0.2 to 1,000 μL
    • Calibrated 1-channel and 8-channel pipetting tools
    • Optical sensor for touch-free detection of liquids, labware, tips
    • Compatible with almost all tube sizes (0.2 mL to 50 mL) and microplates with up to 384 wells
    • Compact design to fit into most cleanbench types
    • Intuitive and flexible drag and drop based software
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
Cell cultivation

Cell cultivation

Our Cell Culture Consumables are developed to facilitate the lab work while offering safety and convenience in handling. With our comprehensive range of bioprocess solutions cells can be cultivated under controlled conditions, e.g. using single-use vessels.

 

 

  • Bioreactors
    • Rigid wall stirred-tank single-use vessels covering working volumes from 100 mL - 40L
    • Wide range of industry standard autoclavable glass bioreactors
    • Stainless steel sterilize-in-place vessels
  • Bioprocess Systems
    • Comprehensive and scalable hardware and software solutions for R&D, process development, pilot, and production
    • Small scale and scale down models, parallel operated bioreactors, bench scale solutions, and sterilize-in-place systems
    • Covering working volumes of 60mL - 520L
  • CO2 Incubators
    • 48 to 170 L models, partly with integrated shaker
    • NEW: CellXpert® CO2 incubator family
    • High temperature disinfection (HTD) included
    • Fanless design and deep-drawn chamber for easy and reliable contamination prevention
    • Benchtop, Under Bench, Floor or Stackable
  • Pipettes and Tips
    • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid an accidential tip ejection a clear haptic feedback is provided.
    • The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
    • The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
    • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids or other external conditions without the need for a full calibration.
    • The quick lock enables an easy cleaning and maintenance of your pipette.
Cell manipulation

Cell manipulation

The TransferMan® 4 and InjectMan® 4 have been developed for precise micromanipulation of adherent and suspension cells. Application specific user profiles simplify the individual workflow process and the DualSpeed joystick combines direct and dynamic movement control.

 

  • Micromanipulators
    • Maximum stability ensures vibration-free work
    • One-hand operation for capillary and angle settings
    • Programmable Z-axis limit for preventing capillary breakage
    • Connection with Eppendorf PiezoXpert® and electronic microinjectors
    • Can be adapted to all conventional microscopes
  • Microinjectors
    • Very simple and clearly arranged operation for routine tasks
    • Injection volume ranges from femtoliters to microliters
    • Inject and Clean function can be triggered right on the device using the included mouse or optional foot control
    • Programmable injection guarantees high reproducibility
    • Connection to TransferMan® 4r, InjectMan® 4 or InjectMan®NI 2 enables semi-automatic microinjection
  • Capillaries
    • VacuTips for holding of suspension cells
    • TransferTips for stem cell transfer etc.
    • Femtotips and Femtotip II for microinjection of minimal volumina
    • Piezo-Drill Tips for piezo-assisted micromanipulation
  • Centrifuges
    • Max. rotor capacity: 48 x 1.5/2.0 mL, 6 x 50 mL, 2 x MTP
    • Max. speed: 30,130 x g (17,500 rpm)
    • Remarkable versatility with 12 different rotors
    • Soft-touch one-finger lid closure for ergonomic operation
    • Menu-driven, multi lingual operation menu (English, German,
      French, Spanish) with large backlit display
Analysis & Detection

Analysis & Detection

With the Eppendorf Detection equipment molecular analysis such as nucleic acid and protein quantification, kinetic studies, ELISA, fluorescence or microvolume measurements can be performed.

  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
  • Reader Plates
    • UV-VIS microplates feature an ultrathin film bottom for excellent light transmission in the UV range
    • Black Microplates offer an excellent signal-to-noise ratio - for clear signals even with low-concentration samples
    • White Eppendorf Microplates are optimized for highest sensitivity
      in the detection of luminescence signals by maximizing reflection
    • Solid black and white assay plates are made of polypropylene resulting
      in high resistance to chemicals, mechanical stress and temperature extremes
    • All plates are optimized for minimal autofluorescence and autoluminescence
  • Thermocyclers
    • A beautiful touchscreen to use, or advanced software control
    • Heating rates of up to 10°C/s
    • Great homogeneity – and the tools to show this
    • CycleManager X50 Software – Control and monitor up to x50 cyclers from a central server
    • 2D-Gradient – Taking traditional optimization principles into a new dimension
  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
Storage

Storage

The Eppendorf freezers meet the highest standards for quality in regard to performance, sample security, and convenience. To concentrate your samples prior to the storage, the Concentrator plus is available as well as different sizes and qualities of tubes and plates.

  • Freezer
    • Quick pull-down and recovery times with reliable heavy-duty compressors for sample safety
    • Left- or right-handed outer door as option for optimal space usage in the lab
    • Easy-to-read, eye level, flush mounted control panel and alarm status
    • Inner doors are insulated and sealed, minimizing cold air loss while accessing your samples
    • Special low-temperature outer door seals allow gaskets to remain flexible at -86 °C and prevents ice buildup

       

  • Deepwell plates
    • OptiTrack® matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with
      heat sealing
    • Easy and reliable stacking
  • Conical Tube
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high.
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity.
     

       

  • Sealing Options
    • Storage Film and Foil glued seals for simple and dependable sealing
      during sample storage
    • Heat Sealing Film and foil for continuous locking with the best
      evaporation protection (see heat sealing materials)
    • Foil products are made of aluminum, can be pierced, and protect
      light-sensitive samples
    • Film products are transparent and provide protection against
      unwanted punctures
    • Eppendorf Plate Lid: stable, flexible protection of samples during
      short-term storage

       

  • Concentrator
    • Comes in 3 system configurations
    • Extremely quite operation <50 dB(A)
    • Chemical-resistant, maintenance-free PTFE diaphragm pump eliminates the need for changing up pump oil
    • Small footprint saves valuable bench space
    • Wide variety of 15 different rotor options to accommodate tubes
      from 0.2 mL to 50 mL, microplates and PCR plates