Protein subunit vaccines
Protein subunit vaccines (e.g., for influenza, hepatitis B and C) are a proven strategy for decades and still today in the spotlight for pandemic management.
➕ Require no production of the whole pathogen because the vaccine consists of immunogenic viral or bacterial fragments generated by recombinant protein techniques. Since non-pathogenic, they are considered as safe. Multiple expression systems for antigenic protein components such as yeast, bacteria, insect, mammalian, and plant cells, allow the production of large quantities and to address unmet needs.
➖ Establishment of a proper scale-up of antigen production can be a major constraint. Several booster doses and adjuvants are needed.
Virus-like particle vaccines
Virus-like particle vaccines are self-assembled viral structural proteins that mimic the native virus. This feature enables to trigger strong immune responses.
➕ Lacking the viral genome, the safety profile is excellent. The production is scalable and safe as no living virus or inactivation steps are involved.
➖ The particle assembly is sometimes challenging, the manufacturing process complicated.
Safe and consistent culturing of cells
The CellXpert® C170i offers fast temperature and CO2 recovery (< 5 min/s) and very stable environmental conditions for (e.g) virus host cells as well as reliable contamination prevention (e.g. 180 °C sterilization).
Increase efficiency and downstream quality
The CR22N High Speed Centrifuge with up to 58,700 x g speed enables the pelleting of small insoluble particles to assure the quality of downstream applications. Triangular bottles can speed up harvesting after protein-antigen expression.
Speed up protein characterization assays
The Eppendorf Xplorer® enables quick filling of plates using the dispensing mode. When connected to the Pipette Manager, an even quicker operation of the pipette is possible.
Protect your cells for protein-antigen production
The New Brunswick S41i Incubator Shaker Design is ensuring minimum evaporation of media, stable conditions, and constant high humidity. Additionally, the high-temperature disinfection the seamless chamber design protect your cells during your initial R&D steps of overexpression optimization.
Maximize antigen retrieval
The LoBind® material guarantees maximum retrieval of proteins and nucleic acids. This improves the
reproducibility of sensitive assays, e.g. during antigen characterization. No coating further minimizes the risk
of downstream assay interference.
Automate your repetitive, routine tasks.
With the Eppendorf epMotion® you can use a variety of kits. The epMotion has an intuitive drag-and-drop-based software and full consumable flexibility.
Constant antigen expression conditions
The BioFlo® 320 Bioprocess Controller offers an integrated Scale-up Assist software to facilitate uncomplicated and intuitive upscaling from R&D to production scale.
*All products are for Research Use Only (RUO).
Traditional vaccines: Inactivated and live-attenuated vaccines
Whole inactivated vaccines, first introduced in 1869 for typhoid, still carry the full repertoire of immunogenic components of the original pathogen.
The most novel vaccines: Nucleic acid vaccines
Nucleic acid vaccines are made up of mRNA or plasmid DNA encoding viral antigenic components to express specific proteins of a pathogen after injection. The advantages are manifold.
