Primary samples are usually whole-blood samples. In most cases, plasma (liquid blood components without any cells) or serum (plasma without fibrinogens) is harvested by centrifugation. These samples are then transferred to plates or tubes.
Centrifugation
- Key for dividing solid from liquid blood components
- A wide variety of centrifuges and adapters allow you to handle blood samples in different quantities and vessels
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Manual Liquid Handling
- Mechanical or Electronic Single Channel Pipettes are ideal for precise collecting of supernatant (plasma, serum) without disturbing the pellet
- Filter Tips assure the sample integrity of primary samples
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Plates and Tubes
- Leachable free plates and tubes for optimal processing and storage of samples
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Storage and Management
- Serum or plasma can be stored in high-quality Cryo tubes
- An Ultra-Low Temperature Freezer allows long term storage of whole blood, plasma or serum
- The eLabJournal® and eLABInventory allow for effortless archiving and documentation
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To process the collected samples, samples need to be transferred to tubes or plates.
Manual Liquid Handling
- Mechanical or Electronic Single Channel Pipettes are ideal for precise reformatting of samples from primary vessels
- Filter Tips assure the sample integrity of primary samples
More about Manual Liquid Handling
Automated Liquid Handling
- Benchtop Pipetting Robots support you to deal with an increased sample throughput
- 96-channel head step automation is a very efficient tool to copy plates quickly
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Plates and Tubes
- Leachable free plates and tubes for optimal processing and storage of aliquoted samples
More about Laboratory Consumables
Setting up the ELISA involves adding the samples to the epitope or antibody-coated plates and then adding antibodies/secondary antibodies. The concentration of antibodies in the sample can then be measured by detecting a colorimetric or fluorescent marker. The whole process involves several washing and drying steps.
Manual Liquid Handling
- Single Channel Pipettes are ideal for adding samples from tubes
- Multichannel pipettes can save time when processing ELISA plates and adding and removing reagents and washing buffer
- A Dispenser and Combitips® help add reagents in a quick and precise way. They also help minimize handling steps, used materials and reduce the risk of cross-contamination.
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Automated Liquid Handling
- Benchtop Pipetting Robots and 96-channel head pipettors allow efficient addition of buffers for washing steps
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Tempering and Mixing
- ThermoMixers are especially helpful for mixing reagents and samples in a plate format
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This step describes the actual photometric ELISA measurements. After the measurements, the samples can be stored for further testing.
Storage and Management
- Plates and tubes can be stored using Ultra-Low Temperature Freezer
- The eLabJournal® and eLABInventory allow for effortless archiving and documentation
More about Sample storage solutions