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DASbox® Mini Bioreactor System
Product Information
The DASbox Mini Bioreactor System is a parallel bioreactor system for research and process development in small working volumes. It can operate up to 24 glass or BioBLU® Single-Use Bioreactors in parallel and is suitable for microbial fermentation and cell culture, including the cultivation of stem cells. With working volumes from 60 mL to 250 mL it is an optimal tool for efficient process development, Design of Experiments (DoE) approaches, and scale-down applications.You will find additional download material at the bottom of this page
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Product Information
Technical Data
Applications
Features
Product Information
The DASbox Mini Bioreactor System enables highly efficient process development by allowing precise control of critical process parameters, rapid comparison of numerous experimental conditions, reduction of medium costs, and optimal utilization of all generated data.
• Small working volumes save resources.
• Parallel operation of up to 24 bioreactors saves time and space and improves reproducibility.
• Easily convertible from single-use to reusable bioreactors and vice versa.
• DASware® software suite enables precise monitoring and control of critical process parameters, as well as the implementation of tailored feedback loops, feeding strategies and other features.
• Seamless integration with the Bioprocess Autosampler allows for automated sampling 24/7 to gain complete datasets.
• Connects to BioNsight® cloud software for remote process monitoring and simple, remote analysis of all data sets to make informed decisions.
Operate small bioreactors in parallel
• The DASbox Mini Bioreactor System is designed as a 4-fold system that is expandable to up to 24 parallel operating bioreactors.
• A 24-fold DASbox system takes up only 1.8 m (6 ft) of bench space. This corresponds to 7.5 cm (3 in) per vessel.
• The setpoints, control strategies, and inoculation time of each bioreactor can be controlled individually from one software installation. Like this, multiple experimental conditions can be tested in parallel, for example in a Design of Experiments (DoE) approach.
Automate bioprocess sampling
• The DASbox system can be seamlessly integrated with the Bioprocess Autosampler, which takes samples from multiple bioreactors 24/7 in short and regular intervals and stores them tempered for later analysis.
• The Bioprocess Autosampler can be installed with new as well as retrofitted to existing bioreactor control systems.
Cultivate various cell types, including stem cells
• The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications.
• Glass and single-use bioreactor variants are available, which are optimized to support the special needs of stem cells, such as low-shear mixing and aggregate formation.
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Technical Data
Specifications | DASbox® Cell Culture | DASbox® Cell Culture Single-Use | DASbox® Microbiology | DASbox® Microbiology Single-Use |
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Application | Cell culture | Cell culture | Microbiology | Microbiology |
Number of parallel units | Up to 24 | Up to 24 | Up to 24 | Up to 24 |
Software | DASware control, other DASware optional | DASware control, other DASware optional | DASware control, other DASware optional | DASware control, other DASware optional |
User Interface | Process computer with monitor | Process computer with monitor | Process computer with monitor | Process computer with monitor |
Typical power consumption (4-fold system without process computer) |
168 W (at 230 V)/ 154 W (at 115 V) |
168 W (at 230 V)/ 154 W (at 115 V) |
168 W (at 230 V)/ 154 W (at 115 V) |
168 W (at 230 V)/ 154 W (at 115 V) |
Dimensions (W × D × H, 4-fold system without process computer) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
30 x 70 x 49 cm (12 x 28 x 19 in) |
Product weight | 44 kg | 44 kg | 44 kg | 44 kg |
Typical weight | 44 kg | 44 kg | 44 kg | 44 kg |
Bacteria/Yeast/Fungi | yes | yes | yes | yes |
Mammalian/Animal | yes | yes | yes | yes |
Insect | yes | yes | yes | yes |
Interchangeable Vessels | yes | yes | yes | yes |
Validatable | no | no | no | no |
Stem cells | yes | yes | yes | yes |
Bioreactors |
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Vessels | Glass vessels | Single-use vessels | Glass vessels | Single-use vessels |
Sterilization | Autoclavable | Pre-sterilized | Autoclavable | Pre-sterilized |
Total volume | 350 mL | 380 mL | 350 mL | 380 mL |
Agitation |
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Drive | Direct overhead drives | Magnetic overhead drives | Direct overhead drives | Magnetic overhead drives |
Speed ranges | 20 – 2500 rpm | 20 – 500 rpm | 20 – 2500 rpm | 20 – 2000 rpm |
Impellers | Marine | Pitched-blade | Rushton-type | Rushton-type |
Gassing |
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Gas supply | TMFC; overlay and/or sparger | TMFC; overlay and/or sparger | TMFC; sparger | TMFC; sparger |
Standard gas flow rates | 0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO2 | 0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO2 | 0.2 – 25 sL/h, 0.2 – 18 sL/h CO2 | 0.2 – 25 sL/h, 0.2 – 18 sL/h CO2 |
Standard gas mixing | Air, N2, O2 and/or CO2 | Air, N2, O2 and/or CO2 | Air, N2, O2 and/or CO2 | Air, N2, O2 and/or CO2 |
Feeding |
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Feed lines per vessel |
2 (standard)/ 4 (optional) |
2 (standard)/ 4 (optional) |
2 (standard)/ 4 (optional) |
2 (standard)/ 4 (optional) |
Standard feed rates (depending on tube diameter) | 0.3 – 9.5 mL/h to 13 – 420 mL/h | 0.3 – 9.5 mL/h to 13 – 420 mL/h | 0.3 – 9.5 mL/h to 13 – 420 mL/h | 0.3 – 9.5 mL/h to 13 – 420 mL/h |
Monitoring and Control |
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Temperature control | Liquid-free heating and cooling (Peltier) | Liquid-free heating and cooling (Peltier) | Liquid-free heating and cooling (Peltier) | Liquid-free heating and cooling (Peltier) |
Standard temperature range (at 25° C room temp.) | 10 – 60°C | 10 – 45°C | 10 – 60°C | 10 – 45°C |
pH control | CO2/base, and other set-ups | CO2/base, and other set-ups | Acid and/or base, and other set-ups | Acid and/or base, and other set-ups |
DO control | Cascade (O2 concentration, gas flow rate), and other set-ups | Cascade (O2 concentration, gas flow rate), and other set-ups | Cascade (agitation speed, O2 concentration, gas flow rate), and other set-ups | Cascade (agitation speed, O2 concentration, gas flow rate), and other set-ups |
ORP (redox) measurement | - | - | Optional (select redox or level) | Optional (select redox or level) |
Level/foam | Optional | Optional | Optional (select redox or level) | Optional (select redox or level) |
OD measurement |
Optional (DASGIP OD4) |
Optional (DASGIP OD4) |
Optional (DASGIP OD4) |
Optional (DASGIP OD4) |
Exhaust condensation | Liquid-free (Peltier) | Liquid-free (Peltier) | Liquid-free (Peltier) | Liquid-free (Peltier) |
Exhaust analysis | - | - |
Optional (DASGIP GA4) |
Optional (DASGIP GA4) |
Applications
- Process development in cell culture and microbiology
- Controlled cultivation of stem cells
- Design of Experiments (DoE)
- Media optimization
- Clone and cell line screening, strain characterization
Features
- Parallel set-up of up to 24 bioreactors
- Excellent scalability and reproducibility in both microbial and cell culture applications
- Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as BioBLU 0.3 Single-Use Bioreactors
- Small working volumes save on the amount of cell material, medium and supplements required
- Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel
- Individual temperature control with liquid-free heating and cooling (Peltier)
- Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode
- LC display with key process parameters and integrated alarm function simplifies monitoring
- Fully mass flow-controlled gas mixing with individual gas mixture from air, N2, O2, and CO2, each directable either to headspace or sparger
- Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available
- Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h
- Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable
- Optional pull-out system for enhanced accessibility of bioreactors and control unit
Downloads: DASbox® Mini Bioreactor System
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