Centrifugation Applications

Lab Academy 22. juillet 2019

Centrifuges are used for many separation processes in the laboratory. The centrifuge works on the sedimentation principle: substances are separated according to their density under the influence of g-force. Depending on the sample type, there are several centrifugation methods available. Examples include isopycnic, ultrafiltration, density gradient, phase separation, and pelleting.

Workflows

One of the most common methods of centrifugation is pelleting, which is used for many popular purification protocols, such as:

DNA extraction
Plasmid DNA extraction (mainly bacteria or yeast)
Protein extraction
Extraction of blood cells

You can find some typical application protocols in our "basics in centrifugation" section. In each case, centrifugation, centrifuge accessories, centrifuge function and rotor specifications are stated.

References:
Trizol^® is a registered trademark of Fisher Scientific *:

https://tools.thermofisher.com/content/sfs/manuals/trizol_reagent.pdf

DNA extraction - typical workflow via kit purification

PDF 0,14 MB

Plasmid Mini-Prep* from E.coli over night culture (5mL)

PDF 0,09 MB

Plasmid Maxi-Prep from E.coli overnight culture* (Commercial Kit protocol)

PDF 0,09 MB

Protein extraction using TRIzol® Reagent*

PDF 0,12 MB

Preparation plasma, platelet, serum

PDF 0,09 MB

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Centrifugation Applications

Workflows

Workflows

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