Introduction to Bioprocessing

Bioprocess Basics & Hands-on

Cultivation in bioreactors enables efficient and reproducible production of cells, microorganisms and the products they express. Applications and used cell lines and strains are diverse and therefore the optimal bioreactor design and process setup differs. On this page, we provide background information, examples, and tips and tricks, to support you in setting up your bioprocess.

Resolving cultivation bottlenecks

For many applications, the cultivation of bacteria or yeast in shake flasks and cell culture in dishes or T-flask is well suited. However, the use bioreactors and fermentors improve the results and save work, time, and lab-space of scientists, who

need large amounts of cells, microbes or of the products they express
would like to increase the reproducibility of growth, product formation or product quality
would like to systematically compare different growth conditions
would like to increase the cultivation efficiency

Bioprocess fundamentals

What are bioreactors and fermenters? Why can they help saving work, time, and lab-space and improving results? How does cultivation of cells and microbes in bioreactors differs from growth in incubators and shakers?

Find the answers in our White Paper!

Download whitepaper (PDF)

Moving from Shakers to Bioreactors

Shake flasks, cell culture dishes, and T-flask first come to mind, when thinking about cultivation systems for growing eukaryotic and prokaryotic cells in the lab. Bioreactors and fermenters are an alternative to consider, if large amounts of cells are needed, the efficiency of the cultivation or its reproducibility need to be enhanced.

Article:
Can your laboratory meet the demands of today's bioprocessing
Application Note:
Optimal bioreactor inoculum preparation in shake flasks
Podcast:
From Shaker to Bioreactor
or Interview:
From Shaker to Bioreactor

eBooks

Bioprocessing Basics

Do you want to learn more about the key characteristics of a bioreactor and the differences between batch, fed-batch, and continuous fermentation modes? Then download our free eBook in which we present basic bioprocessing topics.

Resolving Cultivation Bottlenecks: The Bioprocessing Journal

Ever questioning yourself about how bioreactors can boost reproducibility? Download your free eBook in which we present basic bioprocessing topics including an illustration of the basic components of a bioreactor.

Expanding Cell Growth Capabilities

How to optimize cell growth in shakers, incubators and bioreactors will be answered in our eBook “Expanding Cell Growth Capabilities”. Download your free sample.

Bioreactor accessories

Bioreactors are equipped with accessories like sensors, impellers, and gassing devices. These devices facilitate precise control of the culture’s pH, temperature, oxygen concentration, and more. Like that optimal growth conditions can be created. Get to know some details!

Article:
A guide to impeller selection
Short protocol:
Dissolved oxygen sensors – functioning and calibration
Short Protocol:
Measuring oxygen transfer rate (OTR)
FAQs:
Bioreactor accessories

Culture feeding

Replenishing nutrients during the cultivation improves the growth of cells and microorganisms. Get to know why and how!

Application Note:
A beginner’s guide to bioprocess modes
Article:
Making the most out of your working volume

Optimize Your Bioprocess

In this webinar, our colleagues Ines Hartmann and David Solbach will depict the typical applications of shakers and bioreactors in an upscaling bioprocess. They will introduce to you the basic components of a bioreactor and the various process parameters that can be controlled. Finally, they will go through different process strategies to further optimize a process.

From Shaker to Fermenter

In this webinar, our colleague Jörg Schwinde shows the differences between the cultivation of cells in shakers and bioreactors. We will focus on the basic components of a bioreactor and the various process parameters that can be controlled. Finally, we will focus on different process strategies to further optimize a process and increase.

Cells in Motion

In this webinar, our colleagues Ines Hartmann and Ankita Desai look at the biophysical background of both cultivation techniques. They show how to optimize your process in shake flasks and bioreactors via proper choice of vessel type, vessel material, instrument configuration, bioprocess feeding strategies and parameter settings.

Process parameter setpoints

You would like to get started culturing your favorite cell line or microorganism in a bioreactor and wonder which setpoints to choose? Information from literature can serve as starting point you can build on.

Short Protocol:
E. coli culture in small stirred-tank bioreactors
Short Protocol:
CHO cell culture in small stirred-tank bioreactors
Short Protocol:
Pichia pastoris fermentation in single-use stirred-tank bioreactors
Application Note:
Introduction to Sf9 insect cell culture in stirred-tank bioreactors
Application Note:
Automatic fermentation with the BioFlo 120 Auto Culture Mode

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