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Optimize Your Inoculum Preparation

In this White Paper, our experts review crucial parameters for the optimization of mammalian cell inoculum preparation in shake flasks and in stirred-tank bioreactors. We further discuss challenges, troubleshooting strategies and solutions concerning seed trains of mammalian suspension cell lines like CHO- or HEK293.

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Shake Flask Culture of Mammalian Cells – How to Solve Common Challenges

Despite their ubiquitous nature and simplicity, scientists often encounter a range of different challenges that can seriously hinder the health, productivity, and longevity of their mammalian shake flask culture. Here, we will review common challenges like contamination, cell clumping, poor cell growth, or cell death and provide practical solutionsto help you to overcome them.

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