White Paper: Optimize Your Inoculum Preparation

Optimize Your Inoculum Preparation

Mammalian suspension cell lines, like CHO- or HEK293 cells are widely used host cells for the manufacturing of recombinant therapeutic proteins, antibodies, and viral vectors in the biopharmaceutical industry. In any case, an inoculum or seed culture is required, that provides sufficient cells to ensure efficient culture growth in the final production step.

In this White Paper, our experts review crucial parameters for the optimization of the inoculum preparation both in shake flasks and in stirred-tank bioreactors. We further discuss challenges, troubleshooting strategies and solutions concerning seed trains.