What is reverse pipetting using an air-cushion pipette? Is there literature available?

Unlike forward pipetting, reverse pipetting involves pressing the pipette button to the second pressure point, allowing a greater volume to be aspirated into the tip. When dispensing liquid, there’s no pipette blow-out; instead, the residual liquid remains in the tip. This technique offers enhanced accuracy and precision for pipetting liquids with high viscosity or those prone to foam or produce bubbles. Eppendorf offers a variety of literature explaining the technique and proving application examples, such as the Userguide 020, several webinars, and YouTube videos.

What is pre-wetting using an air-cushion pipette, and is it volume-independent?

Regardless of the volume, the air cushion should be wetted by multiple filling and emptying of the tip before dispensing.

What are effective techniques for pipetting viscous liquids?

When pipetting viscous liquids, we recommend positive displacement instruments such as the Multipette (M4, E3, E3x). Those instruments offer the best dosing accuracy for viscous liquids due to the piston integrated into the tip. When handling viscous liquids with an air-cushion pipette (Research plus, Reference 2), be sure to pipette slowly and use reverse pipetting (see Userguide 020 or our extensive eBook on www.eppendorf.com/Pipetting-eBook for guidance). Additionally, adjust your pipette settings to account for differences in density by using the temporary adjustment. However, results will not match those achieved with a positive displacement device.

Are your pipettes free of nucleic acids?

We cannot guarantee that our pipettes are completely free of nucleic acids. While we maintain high standards of cleanliness and care during production, we do not implement specific measures to prevent nucleic acid contamination. To protect your samples from potential contamination, we recommend using positive displacement devices or filter tips, such as ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax.

When should I grease my Eppendorf pipettes?

The piston or cylinder in the lower part of the pipette should be relubricated after cleaning, decontamination, or exposure to grease-dissolving solutions such as organic solvents. Re-greasing your pipette after autoclaving is not necessary.

How should warm or cold liquids be pipetted?

Best Practices for Pipetting Warm or Cold Liquids: Warm and cold liquids can behave differently than those at room temperature, requiring adjustments in pipetting techniques. Temperature differences between the liquid and the pipette can affect the air cushion, resulting in inaccuracies and imprecision that may exceed permissible error limits. To achieve optimal results, ensure that the pipette, tips, liquid, and surrounding air are all at the same temperature. For enhanced accuracy and precision, consider using a positive displacement device like the Multipette M4 or E3/E3x.

Can the heat of my hand affect my pipetting result?

Modern pipettes address this issue by ensuring sufficient spatial separation between the piston and the handle. Additionally, the materials used in our pipettes support thermal shielding/isolation to prevent heat transfer from your hand (see Userguide 021).

Do Eppendorf pipettes always have to be kept in a stand or can they also be put down on the laboratory bench?

The pipette can be placed on the laboratory bench, but never with liquid inside the tip. In general, it is best to keep pipettes on the pipette carousel. When storing pipettes in a drawer, ensure the pipette does not come into contact with the drawer walls or other equipment, as this may damage the pipette when the drawer is opened or closed.

How can I adjust my air-cushion pipette for challenging liquids, long tips, or higher/lower altitudes?

Upon manufacturing, pipettes are calibrated to distilled water. Eppendorf mechanical pipettes allow temporary user adjustments, beneficial for liquids with different physical properties than water (density, viscosity, surface tension, vapor pressure), or when capillary actions occur (e.g., with DMSO). Adjustments are also beneficial when atmospheric pressure changes due to altitude or when using non-standard pipette tips (e.g., epT.I.P.S. long). The adjusted pipette delivers the correct volume only for the liquid used during adjustment. Please be aware that variable mechanical pipettes turn into "fixed-volume pipettes" when being adjusted. That means they can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. Research plus and Reference 2 pipettes feature an adjustment window where the change can be monitored. It can be set back to factory settings "0". For detailed procedures and examples, refer to the operating instructions and Application Note No. 198. In contrast to mechanical pipettes, the electronic Xplorer/Xplorer plus pipettes can be adjusted for the entire range of volume. Additionally, the electronic pipettes offer several options for adjusting the pipette in the "Options" menu, e.g. to 75 % ethanol, altitude, elongated tips. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings. For more information see User Guide 036. Temporary user adjustments can be reset to manufacturer settings without recalibration.

How do I determine the volume values when calibrating gravimetrically?

The most common method involves measuring the mass of distilled water (whose physical properties are known) using a balance, and then converting the mass to a volumetric value. This means that the measurement values from the analytical balance are multiplied by the correction factor Z (see SOP pipettes). This adjustment accounts for the density of water at the specific temperature and the relevant air conditions.

What does EN ISO 8655 cover?

ISO 8655-2022 is the global standard for piston-operated volumetric apparatus (POVA), including pipettes, burettes, dilutors, dispensers, and manual precision syringes, as defined by the International Organization for Standardization (ISO). It ensures standardized performance for manufacturers, calibration providers, and users.It is structured in 10 chapters: ISO 8655-1: Terminology, general requirements and user recommendations ISO 8655-2: Pipettes ISO 8655-3: Burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric reference measurement procedure ISO 8655-7: Alternative measurement procedures ISO 8655-8: Photometric reference measurement procedure ISO 8655-9: Syringes ISO 8655-10: User guidance, user qualification and POVA qualification.

What do the terms random error and systematic error mean in a pipette calibration?

In pipette calibration, random error and systematic error are two key concepts that help ensure accuracy and precision in measurements. Random Error refers to the variability in measurements when the same volume is dispensed multiple times. It is also known as imprecision and is determined by calculating the repeat standard deviation of 10 measurements. These errors tend to scatter around the true value and can be minimized by consistent technique and proper training. Systematic Error is the consistent deviation of the measured volume from the selected/true volume, also known as inaccuracy and is determined by establishing the mean value of 10 measurements. Systematic errors can arise from defective or misadjusted devices and need to be corrected through proper calibration and maintenance.

At which volumes should a variable pipette be calibrated?

According to the EN ISO 8655-2022 standard, three series of 10 measurements are used to calibrate a variable-volume pipette. The three test volumes are: 1. Nominal volume (the largest volume adjustable by the user, as determined by the manufacturer) 2. 50% of the nominal volume 3. 10% of the nominal volume, or the lower limit if the minimum is over 10%

What is the nominal volume of a variable pipette?

The nominal volume of a variable pipette is always its maximum volume. For a 100 - 1,000 µL pipette, this means 1,000 µL.

I very frequently work with different chemicals. Can I still perform pipette calibration using water?

As per EN ISO 8655-2022, pipette calibration, which involves checking liquid dispensed by the pipette on a balance, should always be performed using distilled or deionized water. If you are working with chemicals that have different properties than water, consider using the temporary adjustment to minimize errors as outlined in Application Note 198. In general, we recommend using direct displacement devices such as the Multipette M4/E3(x) for challenging liquids.

How are multi-channel pipettes tested?

According to ISO 8655-2022, every channel of a multi-channel pipette must be tested. While all channels need to be filled, only the channel being tested is measured. Due to the high number of measurements required (e.g., 240 measurements for an 8-channel pipette and 360 measurements for a 12-channel pipette), it is advisable to send the multi-channel pipette to a professional calibration lab. These labs are equipped with specialized balance setups that ensure all channels are filled while only one channel is measured at a time.

Is the pipette grease free of resins?

Yes, the pipette grease supplied by Eppendorf is free of resins.

How often can I autoclave the Tip-Tub reagent reservoir (Order no. 0030058607), and what conditions should I use?

The Tip-Tub reagent reservoir (approx. 13.8 cm × 5.8 cm × 3.3 cm) is made entirely of polypropylene (reservoir and lid) and is autoclavable. Please note the following: Autoclave conditions: • Autoclave for 20 minutes at 121 °C and 1 bar overpressure. • Do not apply any force or weight on the product during autoclaving Preparation & positioning: • Remove all packaging including plastic foil before autoclaving. • We recommend autoclaving the Tip-Tub lying flat. It can also be autoclaved upside down, stacked, or placed upright in a glass vessel. Number of cycles: • The Tip-Tub can generally be autoclaved multiple times without loss of function. • As with all polypropylene labware, each autoclaving cycle stresses the material, so after repeated autoclaving (e.g., around 20–30 cycles) slight deformations or a “milky” lid may occur. These changes are typically only in appearance and do not affect the intended use of the product.

How many times can Reference 2 be autoclaved?

Our pipettes are checked for a minimum lifetime of five years. This means a normal use with 200,000 strokes, 26,000 volume changes, 100,000 tip ejections, and 175 autoclavings without the pipetting results and pipette material being affected.

The housing of Reference 2 contains different materials. What effects does this have on the pipette during autoclaving?

The Reference 2 is mounted in a way that all materials have room for expansion. Furthermore, only materials are used which are resistant to severe conditions, e.g. autoclaving (pressure, rise in temperature).

Is it possible to deactivate the spring-loaded tip cone of a Reference 2 multi-channel pipette?

Yes. The Reference 2 multi-channel pipettes have a switch on the lower part which deactivates/activates the spring-loaded tip cone.

How often should pipettes be calibrated?

The user determines the frequency of calibration. However, according to EN ISO 8655-2022 Part 1, calibration must be performed regularly and at a minimum, once per year. Routine tests should be performed more frequently, ideally at intervals shorter than those for calibration. The frequency of both calibration and routine tests should be established through a risk assessment. This assessment needs to consider factors such as the frequency of pipette use, the number of users, the aggressiveness of the liquids being dispensed, and the acceptable error limits set by the user. Additionally, any industry- or organization-specific frequency requirements must be adhered to.

What material is my pipette made of and what chemicals is it resistant to?

Please refer to the Operating Manual on our homepage for information on material or chemical resistance before use. The Research plus and Reference 2 have separate chemical resistance sheets. Please note that aggressive chemicals used over a longer period of time may shorten the lifetime of the pipette.

Is it possible to inactivate the spring-loaded tip cone of a single-channel pipette?

Yes, the delivery includes a locking ring to easily deactivate the spring-loaded tip cone. Disassemble the upper from the lower part of the pipette and push the locking ring onto the lower part from the top. Refer to the Operating Manual for an illustrated guide. This is useful for pipette tips not designed for Eppendorf pipette cones.

What is the difference between factory setting and user adjustment?

Eppendorf pipettes offer factory adjustment and temporary adjustment. The temporary adjustment is an easy way of adjusting the pipette temporarily to different conditions (e.g. pipetting a liquid with high density). The factory adjustment should be changed if the pipette was found to be out of specifications during calibration. Before adjusting, exclude other causes such as pipette handling, environmental errors, or worn-out parts.

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Eppendorf Reference^® 2 - Single-Button Pipette

Eppendorf Reference® 2 mechanical single-channel and multi-channel pipettes

Eppendorf Reference® 2 four-digit volume display for easy, quick and accurate volume setting

Eppendorf Reference® 2 single-channel pipette along with 8- and 12-channel choices

Changing volume settings on the precise Eppendorf Reference® 2 pipette is quick and easy

Eppendorf Reference® 2 mechanical pipette in use with a tube

On/Off switch to deactivate spring-loaded tip cones on Eppendorf Reference 2 multi-channel lower part

Eppendorf Reference® 2 single-channel pipette family

Eppendorf Reference® 2 multi-channel pipette family

View of parts for the Eppendorf Reference® 2 mechanical pipette

epT.I.P.S.® macro volume tips facilitate easy reach to the bottom of e.g. 15 mL centrifugation tubes or flasks

The Pipette Carousel 2 with space for up to six Eppendorf pipettes

Laboratory technician using an Eppendorf Reference® 2 mechanical single-button pipette in the lab

Laboratory technician with Eppendorf Reference® 2 mechanical pipette in the lab

Laboratory technician adjusting Eppendorf Reference® 2 one-button pipette in the lab

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Product Information

Choose the Eppendorf Reference 2 mechanical pipette with ergonomic single-button operation and benefit from its high precision and accuracy with reduced operating efforts. Its smooth surface and full autoclavability guarantee efficient decontamination - making it the ideal companion when working under sterile conditions. Available as a single-channel pipette in fixed or variable volume, and as 8- and 12-channel pipettes - all with time-saving single-button operation. Once you have experienced this unique operating philosophy you will not want to change back!

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You will find additional download material at the bottom of this page

Product Information

Technical Data

Applications

Features

A premium pipette that delivers extraordinary precision and accuracy

The Eppendorf Reference 2 mechanical air-cushion pipette with outstanding precision and accuracy is extremely reliable and robust, allowing for a long service life. With its unique single-button operation, the Reference 2 provides fast and ergonomic handling with reduced operating efforts and active aerosol reduction. The clear haptic feedback helps to avoid accidental tip ejection. Don’t compromise on quality and reproducibility – choose the Eppendorf Reference 2 to confidently pipette precious samples accurately and improve your results.
The sturdy handle manufactured with stainless steel protects the pipette from impact and in combination with spring-loaded bumpers, the Reference 2 pipette is likely to stay within calibration even if it takes a fall. These high levels of security make the Reference 2 your ideal companion in applications requiring extremely high levels of accuracy.

Fast and ergonomic single-button operation

With the precise Eppendorf Reference 2 pipette, you can benefit from reduced strain thanks to lower operating forces for aspiration, dispensing and tip ejection. Users can also experience faster operation times as there is no need to move your thumb from side to side to eject tips, meaning you can move quickly through pipetting taks. And as tips are ejected immediately following dispensing, you can benefit from a reduced aerosol contamination of the pipette.

User-friendly temporary adjustment option

It is important to make sure you adjust your pipette according to sample type. The Reference 2 mechanical pipette allows you to do this easily without disturbing factory settings. A temporary and simple adjustment allows for an easy alteration of your Reference 2 to perform accurate pipetting of different liquids. Recommended settings are provided in the manual and you can easily and quickly reset to the manufacturer settings.

The ideal lab companion in sterile work environments

The Reference 2 pipette can easily be taken apart, is fully autoclavable and features a smooth housing being made in one pour. This offers advantages for any researcher concerned about bacterial contamination. With its low-adhesive surface and a minimum of cavities, grooves and sliding parts, the Reference 2 is easy to decontaminate and particularly suitable for use in class A/B cleanrooms and other sterile environments.

You have the choice: we offer a variety of single- and multi-channel options

Not all experiments are based on the same working conditions. Depending on the vessel type, you can choose from single-channel Reference 2 pipettes that are ideal for working with tubes and flasks, and multi-channel Reference 2 pipettes better suited for plates.

• Tubes & flasks: Single-channel choices, available as variable or fixed-volume pipettes
• 96- and 24-well plates: 8- and 12-channel pipette choices with option to deactivate the spring-loaded tip cones.

Fatigue-free and ergonomic pipetting

With it's unique single-button operation, the Eppendorf Reference 2 pipette features an innovative and highly ergonomic operation concept that was designed in line with the Eppendorf PhysioCare Concept ^® to minimize hand and arm strain. As most other Eppendorf pipettes, the Reference 2 premium pipette is of course equipped with the spring-loaded tip cone and SoftEject technologies from Eppendorf.

Spring-loaded tip cones allow for reliable tip fit and effortless attachment

Eppendorf pipettes with spring-loaded tip cone save you time and energy. With haptic feedback, all it takes is one gentle push-down for easy tip attachment. The spring limits the attachment forces and at the same time guarantees a reproducible and safe tip fit. This clever design provides increased user-to-user reproducibility and more consistent results between different lab members. All micropipettes from Eppendorf up to 1,000 µL (except Move It^® 300 µL models) are equipped with the spring-loaded tip cone feature. In all Reference 2 multi-channel pipettes, the springs can easily be deactivated with an "On/Off" switch on the pipette lower part.

SoftEject technology reduces tip ejection forces by 50 %

All non-adjustable multi-channel pipettes from Eppendorf use the innovative SoftEject technology to ensure that during each single tip ejection stroke, the outside tips eject before the inside tips. This is not noticeable to the user but reduces tip ejection forces by 50 %. You just lightly press the ejection button once and the tips are automatically ejected in succession.

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Technical data and specifications of Eppendorf Reference^® 2 single-button pipettes, including the number of channels, volume ranges, random and systematic error, autoclavability, and more.

Specifications	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2	Eppendorf Reference^® 2

Product type	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes
Pipetting type	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system
Number of channels	1-channel	1-channel	8-channel	12-channel	1-channel	1-channel	1-channel	8-channel	12-channel	1-channel	1-channel	8-channel	12-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel
Volume range	0,1 – 2,5 µL	0,5 – 10 µL	0,5 – 10 µL	0,5 – 10 µL	2 – 20 µL	2 – 20 µL	10 – 100 µL	10 – 100 µL	10 – 100 µL	20 – 200 µL	30 – 300 µL	30 – 300 µL	30 – 300 µL	100 – 1 000 µL	0,25 – 2,5 mL	0,5 – 5 mL	1 – 10 mL	1 µL	2 µL	5 µL	10 µL	10 µL	20 µL	20 µL	25 µL	50 µL	100 µL	200 µL	200 µL	250 µL	500 µL	1 000 µL	2 mL	2,5 mL
Color code	dark gray	medium gray	medium gray	medium gray	light gray	yellow	yellow	yellow	yellow	yellow	orange	orange	orange	blue	red	violet	turquoise	dark gray	dark gray	medium gray	medium gray	yellow	light gray	yellow	yellow	yellow	yellow	yellow	blue	blue	blue	blue	red	red
Volume increment	0.002 µL	0.01 µL	0.01 µL	0.01 µL	0.02 µL	0.02 µL	0.1 µL	0.1 µL	0.1 µL	0.2 µL	0.2 µL	0.2 µL	0.2 µL	1 µL	0.002 mL	0.005 mL	0.01 mL	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−
Operating mode	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical
Spring-loaded tip cone	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	no	no	no	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	no	no
Random measurement error¹⁾	0.1 µL: ± 12%; ± 0.012 µL 0.25 µL: ± 6%; ± 0.015 µL 1.25 µL: ± 1.5%; ± 0.01875 µL 2.5 µL: ± 0.7%; ± 0.0175 µL	0.5 µL: ± 5%; ± 0.025 µL 1 µL: ± 1.8%; ± 0.018 µL 5 µL: ± 0.8%; ± 0.04 µL 10 µL: ± 0.4%; ± 0.04 µL	0.5 µL: ± 8%; ± 0.04 µL 1 µL: ± 5%; ± 0.05 µL 5 µL: ± 2%; ± 0.1 µL 10 µL: ± 1%; ± 0.1 µL	0.5 µL: ± 8%; ± 0.04 µL 1 µL: ± 5%; ± 0.05 µL 5 µL: ± 2%; ± 0.1 µL 10 µL: ± 1%; ± 0.1 µL	2 µL: ± 1.5%; ± 0.03 µL 10 µL: ± 0.6%; ± 0.06 µL 20 µL: ± 0.3%; ± 0.06 µL	2 µL: ± 1.5%; ± 0.03 µL 10 µL: ± 0.6%; ± 0.06 µL 20 µL: ± 0.3%; ± 0.06 µL	10 µL: ± 0.7%; ± 0.07 µL 50 µL: ± 0.3%; ± 0.15 µL 100 µL: ± 0.2%; ± 0.2 µL	10 µL: ± 2%; ± 0.2 µL 50 µL: ± 0.8%; ± 0.4 µL 100 µL: ± 0.3%; ± 0.3 µL	10 µL: ± 2%; ± 0.2 µL 50 µL: ± 0.8%; ± 0.4 µL 100 µL: ± 0.3%; ± 0.3 µL	20 µL: ± 0.7%; ± 0.14 µL 100 µL: ± 0.3%; ± 0.03 µL 200 µL: ± 0.2%; ± 0.4 µL	30 µL: ± 0.7%; ± 0.21 µL 150 µL: ± 0.3%; ± 0.45 µL 300 µL: ± 0.2%; ± 0.6 µL	30 µL: ± 1%; ± 0.3 µL 150 µL: ± 0.5%; ± 0.75 µL 300 µL: ± 0.3%; ± 0.9 µL	30 µL: ± 1%; ± 0.3 µL 150 µL: ± 0.5%; ± 0.75 µL 300 µL: ± 0.3%; ± 0.9 µL	100 µL: ± 0.6%; ± 0.6 µL 500 µL: ± 0.2%; ± 1 µL 1.000 µL: ± 0.2%; ± 2 µL	250 µL: ± 1.2%; ± 3 µL 500 µL: ± 0.3%; ± 1.5 µL 1250 µL: ± 0.2%; ± 2.5 µL 2500 µL: ± 0.2%; ± 5 µL	500 µL: ± 0.6%; ± 3 µL 2500 µL: ± 0.25%; ± 6.25 µL 5.000 µL: ± 0.15%; ± 7.5 µL	1.000 µL: ± 0.6%; ± 6 µL 5.000 µL: ± 0.2%; ± 10 µL 10.000 µL: ± 0.15%; ± 15 µL	1 µL: ± 1.8%; ± 0.018 µL	2 µL: ± 1.2%; ± 0.024 µL	5 µL: ± 0.6%; ± 0.03 µL	10 µL: ± 0.5%; ± 0.05 µL	10 µL: ± 0.6%; ± 0.06 µL	20 µL: ± 0.3%; ± 0.06 µL	20 µL: ± 0.3%; ± 0.06 µL	25 µL: ± 0.3%; ± 0.075 µL	50 µL: ± 0.3%; ± 0.15 µL	100 µL: ± 0.2%; ± 0.2 µL	200 µL: ± 0.2%; ± 0.4 µL	200 µL: ± 0.2%; ± 0.4 µL	250 µL: ± 0.2%; ± 0.5 µL	500 µL: ± 0.2%; ± 1 µL	1.000 µL: ± 0.2%; ± 2 µL	2.000 µL: ± 0.2%; ± 4 µL	2.500 µL: ± 0.2%; ± 5 µL
Systematic measurement error¹⁾	0.1 µL: ± 48%; ± 0.048 µL 0.25 µL: ± 12%; ± 0.03 µL 1.25 µL: ± 2.5%; ± 0.031 µL 2.5 µL: ± 1.4%; ± 0.035 µL	0.5 µL: ± 8%; ± 0.04 µL 1 µL: ± 2.5%; ± 0.025 µL 5 µL: ± 1.5%; ± 0.075 µL 10 µL: ± 1%; ± 0.1 µL	0.5 µL: ± 12%; ± 0.06 µL 1 µL: ± 8%; ± 0.08 µL 5 µL: ± 4%; ± 0.2 µL 10 µL: ± 2%; ± 0.2 µL	0.5 µL: ± 12%; ± 0.06 µL 1 µL: ± 8%; ± 0.08 µL 5 µL: ± 4%; ± 0.2 µL 10 µL: ± 2%; ± 0.2 µL	2 µL: ± 3.0%; ± 0.06 µL 10 µL: ± 1.0%; ± 0.10 µL 20 µL: ± 0.8%; ± 0.16 µL	2 µL: ± 5%; ± 0.10 µL 10 µL: ± 1.2%; ± 0.12 µL 20 µL: ± 1%; ± 0.2 µL	10 µL: ± 3%; ± 0.3 µL 50 µL: ± 1%; ± 0.5 µL 100 µL: ± 0.8%; ± 0.8 µL	10 µL: ± 3%; ± 0.3 µL 50 µL: ± 1%; ± 0.5 µL 100 µL: ± 0.8%; ± 0.8 µL	10 µL: ± 3%; ± 0.3 µL 50 µL: ± 1%; ± 0.5 µL 100 µL: ± 0.8%; ± 0.8 µL	20 µL: ± 2.5%; ± 0.5 µL 100 µL: ± 1%; ± 1 µL 200 µL: ± 0.6%; ± 1.2 µL	30 µL: ± 2.5%; ± 0.75 µL 150 µL: ± 1%; ± 1.5 µL 300 µL: ± 0.6%; ± 1.8 µL	30 µL: ± 3%; ± 0.9 µL 150 µL: ± 1%; ± 1.5 µL 300 µL: ± 0.6%; ± 1.8 µL	30 µL: ± 3%; ± 0.9 µL 150 µL: ± 1%; ± 1.5 µL 300 µL: ± 0.6%; ± 1.8 µL	100 µL: ± 3%; ± 3 µL 500 µL: ± 1%; ± 5 µL 1.000 µL: ± 0.6%; ± 6 µL	250 µL: ± 4.8%; ± 1.2 µL 500 µL: ± 1.5%; ± 7.5 µL 1250 µL: ± 0.8%; ± 10 µL 2500 µL: ± 0.6%; ± 15 µL	500 µL: ± 2.4%; ± 12 µL 2500 µL: ± 1.2%; ± 30 µL 5.000 µL: ± 0.6%; ± 30 µL	1.000 µL: ± 3%; ± 30 µL 5.000 µL: ± 0.8%; ± 40 µL 10.000 µL: ± 0.6%; ± 60 µL	1 µL: ± 2.5%; ± 0.025 µL	2 µL: ± 2%; ± 0.04 µL	5 µL: ± 1.2%; ± 0.06 µL	10 µL: ± 1%; ± 0.1 µL	10 µL: ± 1.2%; ± 0.12 µL	20 µL: ± 0.8%; ± 0.16 µL	20 µL: ± 1%; ± 0.2 µL	25 µL: ± 1%; ± 0.25 µL	50 µL: ± 0.7%; ± 0.35 µL	100 µL: ± 0.6%; ± 0.6 µL	200 µL: ± 0.6%; ± 1.2 µL	200 µL: ± 0.6%; ± 1.2 µL	250 µL: ± 0.6%; ± 1.5 µL	500 µL: ± 0.6%; ± 3 µL	1.000 µL: ± 0.6%; ± 6 µL	2.000 µL: ± 0.6%; ± 12 µL	2.500 µL: ± 0.6%; ± 15 µL
Autoclavable	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
Consumables	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®
Open system (third party consumables)	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
Connectivity	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Delivery content	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, epT.I.P.S.^® pipette tips sample bag, Adjustment tool, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Protection filter (x10), Pipette wrench	Pipette, epT.I.P.S.^® pipette tips sample bag, Adjustment tool, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Protection filter (x10), Pipette wrench	Pipette, epT.I.P.S.^® pipette tips sample bag, Adjustment tool, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Protection filter (x10), Pipette wrench	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring to prevent spring action	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Protection filter (x10), Pipette wrench	Pipette, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Protection filter (x10), Pipette wrench

1)The listed values for 10%, 50%, and 100% of the nominal volume are based on tests complying with DIN EN ISO 8655, Parts 2 or 5. If listed, the smallest adjustable volume provides additional data.

Customer voices: Eppendorf Reference^® 2

“These pipettes are very easy to use. The adjustment lock is placed in a location where it is easy to use but so you don't inadvertently press it. The results we obtained were very consistent and very accurate. The pipette mechanism is very smooth and easy to control. They seem very rugged.”

Brad Stratton

Rieke Metals, USA

Write your own review!

Customer voices: Eppendorf Reference^® 2

“As regular users of laboratory equipment, we have come to rely heavily on the accuracy and ease of use provided by Eppendorf pipettes. This brand has become synonymous with precision and dependability, and our experience with their pipettes has undoubtedly reinforced this reputation. […] Eppendorf pipettes have proven to be exceptional tools in our lab. They offer a combination of precision, ease of use, and durability that sets them apart from other brands in the market.”

Jignesh Prajapati

Gujarat University, India

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Customer voices: Eppendorf Reference^® 2

“Accurate, precise, easy to use, and durable pipette. [Our Reference 2] pipette is over 10 years old with yearly maintenance and has only needed o-rings to be changed.”

Joanna Collier

Thermochem, USA

Write your own review!

Downloads: Eppendorf Reference^® 2

Selection Guide

Get It Right! Selection Chart for Liquid Handling Instruments & Tips

Find the most suitable pipetting system for your lab applications

PDF 1.1MB

English

Flyer

Increase Your Pipetting Productivity Today

Higher throughput and increased reproducibility with Eppendorf multi-channel pipettes

You give your all to scientific research every day. Eppendorf liquid handling instruments help you grow beyond your limits

Instruments de manipulation des liquides Eppendorf :
Inégalés en matière de technologie, d’effi cacité et de manipulation – depuis 50 ans.

PDF 8.3MB

French

Brochure

Pipette Service Done Right

Eppendorf Pipette Calibration

PDF 3.0MB

English

Brochure

Service optimal des pipettes

Étalonnage des pipettes par Eppendorf

PDF 2.9MB

French

Flyer

Eppendorf Pipette Multi-Packs (Starter Kits)

Pick your pack and save on Eppendorf pipettes

PDF .7MB

English

Flyer

Elevate Your Laboratory Standards

ISO standards for calibration of liquid handling devices

PDF 1.2MB

English

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Eppendorf Reference® 2 - Single-Button Pipette

Eppendorf Reference® 2 single- and multi-channel pipettes with one-button operation

Eppendorf Reference® 2 four-digit volume display for easy, quick and accurate volume setting

Eppendorf Reference® 2 single-channel pipette along with 8- and 12-channel choices

Changing volume settings on the precise Eppendorf Reference® 2 pipette is quick and easy

Eppendorf Reference® 2 mechanical pipette in use with a tube

On/Off switch to deactivate spring-loaded tip cones on Eppendorf Reference 2 multi-channel lower part

Eppendorf Reference® 2 single-channel pipette family

Eppendorf Reference® 2 multi-channel pipette family

View of parts for the Eppendorf Reference® 2 mechanical pipette

epT.I.P.S.® macro volume tips facilitate easy reach to the bottom of e.g. 15 mL centrifugation tubes or flasks

The Pipette Carousel 2 with space for up to six Eppendorf pipettes

Laboratory technician using an Eppendorf Reference® 2 mechanical single-button pipette in the lab

Laboratory technician with Eppendorf Reference® 2 mechanical pipette in the lab

Laboratory technician adjusting Eppendorf Reference® 2 one-button pipette in the lab

How to Pipette in 5 Simple Steps

The unique single-button operation of the Eppendorf Reference® 2 (Interview with Product Manager)

The premium design of the Eppendorf Reference® 2 (Interview with Product Designer)

The outstanding robustness of the Eppendorf Reference® 2

How the Eppendorf Reference® 2 looks from the inside

The simple adjustment of the Eppendorf Reference® 2

How to Fill Microplates Faster

Multi-Channel Pipette Tip Attachment

How to Take Care of Your Pipette

Userguide : Pipette calibration

Eppendorf Reference® 2 One-Button Pipette

Product Information

A premium pipette that delivers extraordinary precision and accuracy

Fast and ergonomic single-button operation

User-friendly temporary adjustment option

The ideal lab companion in sterile work environments

You have the choice: we offer a variety of single- and multi-channel options

Fatigue-free and ergonomic pipetting

Spring-loaded tip cones allow for reliable tip fit and effortless attachment

SoftEject technology reduces tip ejection forces by 50 %

Technical Data

Applications

Features

Customer voices: Eppendorf Reference® 2

“These pipettes are very easy to use. The adjustment lock is placed in a location where it is easy to use but so you don't inadvertently press it. The results we obtained were very consistent and very accurate. The pipette mechanism is very smooth and easy to control. They seem very rugged.”

Customer voices: Eppendorf Reference® 2

Customer voices: Eppendorf Reference® 2

“Accurate, precise, easy to use, and durable pipette. [Our Reference 2] pipette is over 10 years old with yearly maintenance and has only needed o-rings to be changed.”

Downloads: Eppendorf Reference® 2

Eppendorf Reference^® 2 - Single-Button Pipette

Eppendorf Reference^® 2 One-Button Pipette

Customer voices: Eppendorf Reference^® 2

Customer voices: Eppendorf Reference^® 2

Customer voices: Eppendorf Reference^® 2

Downloads: Eppendorf Reference^® 2