Under which conditions is it recommended to consider protein quantification via fluorescence?
First and foremost, the starting concentration of the sample must be taken into account. If enough sample material of sufficient concentration is available, the colorimetric or the direct quantification methods will be satisfactory. If this is not the case, it is advisable to consider the fluorometric method, despite the additional effort, as it requires considerably less sample material. Quantification of protein mixtures, in particular, will benefit from the low variability between proteins that is afforded by the fluorescence method.