Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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DNA LoBind^® Plates

Small section of Eppendorf DNA LoBind® microplate

Deep well Eppendorf LoBind® plate with blue border

Sample being pipetted from elongated ep T.I.P.S.® to Eppendorf LoBind® deepwell plate

Barcoded Eppendorf LoBind® plate in deep well format

Product Information

Our innovative DNA LoBind technology reduces sample-to-surface binding to ensure maximum nucleic acid recovery and offer the ideal solution for sample preparations and long-term storage.

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You will find additional download material at the bottom of this page

Product Information

Applications

Features

Maximize recovery of nucleic acids with DNA LoBind^® Plates! A combination of special manufacturing technologies and selected polypropylene batches ensure nearly 100 % recovery of DNA/RNA molecules-without surface coating to eliminate the risk of sample contamination. This makes Eppendorf LoBind Plates ideal for applications where sample recovery is key - such as the preparation of long-term storage nucleic acids in forensic, microarrays and NGS applications.

High-quality Eppendorf DNA LoBind^® products

Available in a variety of formats, to help you recover nucleic acids, including tube, microplate and deepwell plate to meet the different application needs for various sample volumes and throughput.

Superior DNA recovery rate

Eppendorf DNA LoBind^® Tubes recover significantly more DNA than competitor tubes. DNA (0.2 ng/µL DNA fragment (130 bp, 32P labelled) in 2.5 M NaCl/TE buffer) was incubated at various conditions before being quantified to assess sample recovery. The Eppendorf DNA LoBind Tubes revealed up to 99 % sample recovery independent of the tested incubation temperature or time.

Get consistency in quantification

Eppendorf LoBind^® Tubes offer greater recovery potential when compared to a low-retention competitor. An assessment of Eppendorf LoBind tube consistency in the preparation and storage of standards for real-time PCR absolute quantifications showed that the low adsorption of DNA to LoBind tubes improved the quality of standard curves and DNA quantification in assayed samples.

Image: Preparation of DNA standards: Genomic DNA stock solution was serially diluted 10–fold in competitor low retention tubes or Eppendorf DNA LoBind^® Tubes. 22 µL aliquots of each dilution were stored for 24 hours at –80 °C before analysis by real-time PCR. Samples stored in Eppendorf LoBind^® Tubes had a greater PCR efficiency when compared to the competitor. See Application Note 226 for more details.

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Downloads: DNA LoBind^® Plates

Flyer

Eppendorf Purity Grades Selection Guide

Quality – Stability – Purity: Eppendorf Plates^®

PDF 4.7MB

English

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High quality single tubes and tube strips. These tubes allow for optimized heat transfer, are easy to open and provide tight sealing to prevent evaporation in PCR. Different formats available.

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DNA LoBind® Plates

Small section of Eppendorf DNA LoBind® microplate

Deep well Eppendorf LoBind® plate with blue border

epT.I.P.S.® L elongated pipette tips in use to fill a Deepwell plate

Barcoded Eppendorf LoBind® plate in deep well format

Eppendorf LoBind® - How it works

Product Information

High-quality Eppendorf DNA LoBind® products

Superior DNA recovery rate

Get consistency in quantification

Applications

Features

Downloads: DNA LoBind® Plates

DNA LoBind^® Plates

High-quality Eppendorf DNA LoBind^® products

Downloads: DNA LoBind^® Plates