Cleaning/Disinfection: What disinfection agent is recommended for the CellXpert CS220 CO2 Incubator Shaker?

The recommended disinfection agent for the CellXpert CS220 CO2 Incubator Shaker is 70% isopropanol or 70 % ethanol. If you intend to use other disinfection agent please contact your local Eppendorf partner. Carefully follow the cleaning procedures described in the operating manual or in the cleaning screen.

Cleaning/Disinfection : Is the CellXpert CS220 CO2 Incubator Shaker easy to clean?

The platform and sub-platform can be removed easily in less than 2 minutes allowing full access to the chamber for wipe disinfection. The proven CellXpert ® design known from the CellXpert CO2 incubators, the seamless stainless-steel chamber, eases fast cleaning procedures.

Contamination prevention : How does the CellXpert CS220 CO2 Incubator Shaker help in preventing contamination?

The CellXpert CS220 CO2 Incubator Shakers design accomplishes the hygienic design according to EN ISO 14159:2008. The CellXpert ® seamless stainless-steel chamber eases fast cleaning procedures and minimizes the risk of a contamination. The integrated 180 °C chamber sterilization routine adds another layer of protection.

Integrated 180 °C chamber sterilization routine : What precautions must be taken and what has to be removed prior a high-temperature sterilization cycle in the CellXpert CS220 CO2 Incubator Shaker?

The preparation steps are guided in the Disinfection screen of the CellXpert CS220 CO2 Incubator Shaker. The relative humidity sensor must be protected prior the high-temperature sterilization with the provided protection cover. Temperature-sensitive accessories made of rubber or containing plastic parts such as sticky pads and test tube racks must be removed.

Integrated 180 °C chamber sterilization routine : What is the maximum temperature that is achieved during the high-temperature sterilization of CellXpert CS220 CO2 Incubator Shaker?

The maximum temperature of the high-temperature sterilization of the CellXpert CS220 CO2 Incubator Shaker is 180°C, with a holding time of 2 hours.

Integrated 180 °C chamber sterilization routine : What is the duration of the whole high-temperature disinfection (HTD) cycle of the CellXpert CS220 CO2 Incubator Shaker?

The duration of the high-temperature disinfection (HTD) cycle of the CellXpert CS220 CO2 Incubator Shaker is approximately 19 hours. This is depending on the set-point and the ambient conditions.

Integrated 180 °C chamber sterilization routine : If running a high-temperature disinfection (HTD) in one device, is it possible to use the other device(s) in a CellXpert CS220 CO2 Incubator Shaker stacked configuration?

If running a high-temperature disinfection (HTD) cycle in one CellXpert CS220 CO2 Incubator Shaker in a stacked configuration, the temperature in the device stacked directly on top may increase temporarily, it is recommended to remove temperature sensitive cells. The device(s) stacked below will not be affected and can be used for cell cultivation.

Integrated 180 °C chamber sterilization routine : Does the CellXpert CS220 CO2 Incubator Shaker logs the high-temperature disinfection (HTD) cycle and is there a possibility to download a HTD protocol?

Yes, the internal chart is logging the temperature. In additon in the end of the high-temperature disinfection (HTD) cycle, the screen of the CellXpert CS220 CO2 Incubator Shaker indicates the result with "disinfection successful". A pdf protocol can be downloaded via the USB port.

Data logging and export : Can performance data be logged and exported from the CellXpert CS220 CO2 Incubator Shaker?

The CellXpert CS220 CO2 Incubator Shaker logs agitation speed, temperature, relative humidity and CO2 as CHART data. Door openings, alarms, events such as set-point changes are logged too in the EVENT log. Chart and event log data is accessible on the User interface and can be downloaded by the USB port [Excel].

Remote Monitoring : Can performance data of the CellXpert CS220 CO2 Incubator Shaker be monitored remotely?

The CellXpert CS220 CO2 Incubator Shaker is a VisioNize OnBoard device and can be connected to the Eppendorf VisioNize Lab Suite (VNLS) for remote monitoring and alarm notifications. For more information on VNLS contact your local Eppendorf partner.

Remote Monitoring : Can the CellXpert CS220 CO2 Incubator Shaker be connected to third party software or monitoring systems?

As a VisioNize ® on-board device, network-based integration of the CellXpert CS220 CO2 Incubator Shaker to e.g., third-party monitoring systems via OPC-DA and JSON REST protocols is possible. It requires a connector/integration software developed by a 3rd-party or IT specialist. The data can readily be explored via an OPC/XML DA client or web-browser (REST). Eppendorf provides a User guide to retrieve data from the interface on request, please contact your local Eppendorf partner. Please note that users and third-party software providers are responsible for integrating the devices into third-party monitoring systems like LIMS to ensure all requirements are being met. We recommend to consult the monitoring software provider regarding their experience with integrating Eppendorf devices.

Maintenance : What maintenance intervals are recommended for the CellXpert CS220 CO2 Incubator Shaker. Are there any parts which should be regularly renewed on the CellXpert CS220 CO2 Incubator Shaker?

Eppendorf recommends having your device inspected and maintained at regular intervals by trained and skilled personnel, e.g. annually. Eppendorf offers customized service solutions for preventive maintenance, qualification and calibration of your device. For information, offers and contact options, visit our website www.eppendorf.com/epservices.

Configuration/Options : What configurations are available for the CellXpert CS220 CO2 Incubator Shaker?

The CellXpert CS220 CO2 Incubator Shaker comes included with temperature (non-refrigerated), CO2 and active relative humidity control. One can select between a 25 or 50 mm orbit (shaking diameter). The orbit is non-adjustable.

Temperature Control : What is the temperature range of the CellXpert CS220 CO2 Incubator Shaker? Is the device available with refrigeration / cooling?

The temperature range of the CellXpert CS220 CO2 Incubator Shaker is 8 °C above ambient up to 50 °C with a setting increment of 0.1 °C. There is no refrigerated option. The CellXpert CS220 CO2 Incubator Shaker can be operated in an ambient temperature between 18 °C - 28 °C.

Operation : Does the CellXpert CS220 CO2 Incubator Shaker comes with a pull-out platform?

Yes, the sample platform can be pulled out for easy access to the samples. The platform is sitting on a robust sub-platform, that can be pulled out. The handle can be operated with one hand. The platform can be easily removed without the use of any addtional tools.

Humidity control : Does the CellXpert CS220 CO2 Incubator Shaker has active or passive relative humidity control?

The CellXpert CS220 CO2 Incubator Shaker has active humidity control. This allows a precise control of the humidity level. The required relative humidity value is set on the User Interface. Typically, this is 85 %.

Humidity control : How does the relative humidification system of the CellXpert CS220 CO2 Incubator Shaker work?

The relative humidity control system of the CellXpert CS220 CO2 Incubator Shakers is active steam humidification: The device is equipped with a water-supply system, that pumps water from an outside sitting water tank via tubing and an additional water filter to the device. In the device a heating cartridge heats the water and vaporizes it to finest hygienic water steam which is led into the chamber. Active steam humidification omits the use of a water bath.

Humidity control: How does the CellXpert CS220 CO2 Incubator Shaker avoids condensation built-up?

If the relative humidity level exceeds the setpoint of 85 % and a risk of condensation occurs, a decrease of the humidity level is achieved by a pump that lets ambient air through a sterile filter into the chamber. Together with the proven CellXpert 6-sided direct heating principle (including heated window) this enables a condensation-free operation of the CellXpert CS220 CO2 Incubator Shaker.

Humidity control : What water must be used for the water tank in the CellXpert CS220 CO2 Incubator Shaker?

Only use ultrapure water with a conductivity of max. 5 µS/cm for the water tank of the CellXpert CS220 CO2 Incubator Shaker. Using tap water may lead to blocking.

Humidity control : What must be ordered to use the CellXpert CS220 CO2 Incubator Shaker with relative humidity control?

The CellXpert CS220 CO2 Incubator Shaker comes with active relative control. The water-supply package (6740070080) contains everything required for set-up for 1, 2 or 3 devices. It includes the water tank (one supplies up to three devices), three connection tubing and filters. The water tank holder can be ordered extra. Replacement tubing and filters are available.

Humidity control : What water quality is recommended for use with the relative humidity system for the CellXpert CS220 CO2 Incubator Shaker?

The relative humidity system of the CellXpert CS220 CO2 Incubator Shaker requires the use of ultra pure water with a conductivity of max. 5 µS/cm (e.g. by reverse osmosis or distilled water). Using tap water will lead to limescale in the vaporizer of the humidification system.

Accessories : What accessory clamps are available for the CellXpert CS220 CO2 Incubator Shaker?

The CellXpert CS220 CO2 Incubator Shaker has dedicated flask clamps. They are made of stainless steel and are 180 °C resistant. They come with metric screws that are included with the clamp and additional spare screws come with the universal platform. Clamp sizes from 100/125 mL – 5L are available. Do only use the specified screws for the device.

Accessories : What accessory test tube racks and microplate racks are available for the CellXpert CS220 CO2 Incubator Shaker?

All Eppendorf test tube racks and microplates fit the CellXpert CS220 CO2 Incubator Shaker platform. Please note that the screws supplied with the racks do NOT fit theCellXpert CS220 universal platform. The delivery package of the CS220 platform contains fitting screws for the test tube and microplate rack (25 pieces). Spare screws are available.

Accessories : Can the CellXpert CS220 CO2 Incubator Shaker universal platform be used with sticky pad?

Yes, the CellXpert CS220 CO2 Incubator Shaker can be used with sticky pads. The sticky pads can be placed directly on the universal platform and fits up to 8 sticky pads to cover the whole platform.

Why should I have my Orbital Shaker serviced at regular intervals?

As with all products with moving parts, wear and tear is inevitable and can affect how consistently the product operates over a long period of time. Eppendorf products are engineered to minimize wear and tear, but a periodic service allows early identification and tracking of excessive wear before product performance is outside of acceptable tolerances. Regular servicing may also be a regulatory requirement of our users.

How often does my orbital shaker need to be serviced?

Eppendorf recommends a service once every 12 months. However, the regulatory requirements of our users vary greatly and can often be more frequent.

How much are the different orbital shaker service programs?

The cost depends on the product variant and on the level of service that is required. Please contact your local Eppendorf Service centre for a personal quotation. www.eppendorf.com/contact

What is IQ or OQ and what do I need them for?

Installation and Operational Qualification; the Installation Qualification documents that the product has been delivered and installed in accordance with manufacturer recommendations and guidelines. The Operational Qualification checks the correct functioning of a device in accordance with manufacturer recommendations and guidelines (as such, it can only be provided by the equipment manufacturer). IQ/OQ is primarily required in organizations that implement quality management processes but is available to all users.

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CellXpert^® CS220 - CO₂ Incubator Shaker

CellXpert® CS220 stainless steel chamber

CellXpert® CS220 single device, door open

CellXpert® CS220 integrated 180 °C sterilization routine

CellXpert® CS220 accessory compatibility

CellXpert® CS220 glide-up door and slide-out platform

CellXpert® CS220 active, bidirectional rH-control

CellXpert® CS220 real-time remote monitoring, alerts and more

Do you want to accelerate your research while minimizing the risk of costly delays for your mammalian suspension cultures? Discover the only CO₂incubator shaker in the market with an integrated 180 °C sterilization and an outstanding capacity-to-footprint ratio. Learn more about the cost- and time-saving features that will help your team remain focused on the tasks critical to your success.

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Accelerate your growth – CellXpert^® CS220 CO₂ incubator shaker

The CellXpert CS220 is a large capacity, stackable CO₂ incubator shaker that was designed to accelerate mammalian suspension culture research projects. It also minimizes the risk of costly project delays by being the first CO₂ incubator shaker in the market that brings the high contamination prevention standards of CO₂ incubator-based cell culture to the shaker world. Discover many cost- and time-saving features below that will help your team to focus on other success-critical tasks.

Main features
• Up to 40 % higher flask capacity: e.g., 8 x 5 L Corning^®/Thomson Optimum Growth^®, 25 x 1 L / 42 x 500 mL / 102 x 125 mL Erlenmeyer flasks (on sticky pad)
• 180 °C sterilization routine integrated: incl. wizard and downloadable performance protocol; no locally restricted UV-airflow/surface-disinfection
• Stainless steel chamber: Hygienic design according to EN ISO 14159:2008 standard plus only few internal parts and no drive wheel, crevices, cover sheets, cables, or heating fins inside
• Optimized throughput per lab space: Highest platform size vs. footprint ratio in the market, stackable up to 3-fold
• Save costs: Always included: slide-out platform, rH-control, touch interface, access port, sterilization routine; no UV-lamps that require frequent exchange; low gas and energy consumption
• Eppendorf X-drive: Proven and tested shaker drive with four weight-supporting shafts for 24/7 vibration-free shaking
• Glide-up door and slide-out platform: Saves space and ensures fast and easy experiment setup, cleaning and service – even at the back of the chamber
• Full control and fast root-cause analysis: 7” touchscreen shows all setpoints and current parameters at a glance; direct visual overview of logged data/events incl. door openings and alarms, user management
• Accessories & options: Storage base and feet, various service options incl. IQ/OQ-GxP-service, VisioNize^® Lab Suite remote monitoring and more
• Limited time special offer: One year free subscription to VisioNize^® Lab Suite Sample Safety+ including SMS notifications, response-based alert escalation, audit trail and more – check availability of VisioNize^® in your country

More throughput per run: Up to 40 % higher flask capacity
Accelerate your research by increasing the culture volume per time and by ensuring same growth conditions for more flasks per run. The extra-large platform (4780 cm²/1882 in²) of the CellXpert CS220 offers more capacity than most other CO₂ shakers in the market.

Capacity examples (sticky pad on universal platform):
• 8 x 5 L Corning^®/Thomson Optimum Growth^®
• 15 x 2 L Corning^®
• 25 x 1 L Erlenmeyer
• 42 x 500 mL Erlenmeyer
• 102 x 125 mL Erlenmeyer

Excellent use of lab space: Highest platform size to footprint ratio in the market
Your lab space is at a premium and you need to make the most out of it? Don't waste an inch of lab space for lab equipment that isn't fully designed for highest throughput per footprint.

Effective contamination risk mitigation: Integrated 180 °C sterilization routine
Contamination is the sword of Damocles always present in a cell culture lab. Inevitably, identification of the contamination source, its removal and repetition of experiments leads to significant binding of lab staff and temporary stop of experiments. In the end, a contamination makes you lose money and valuable time-to-market, especially if it remains undetected like contamination with Mycoplasma.

Almost all current CO₂ incubator shakers use UV-lights that irradiate the airstream or chamber surfaces that are in the line of sight of the UV-light. However, UV-light irradiation is per se locally restricted and does not include all critical areas where media spills and moisture can be expected e.g., underneath the platform. The CellXpert CS220 is the first shaker to apply the established and high contamination protection standards of CO₂ incubator-based cell cultivation – a 180 °C sterilization. And the best part: You don't need to pay extra, it is already integrated.

Don't let contamination get in your way!

Features:
• 180 °C sterilization* instead of mere UV-decontamination of airstream or surfaces
• All chamber components and the platform are included in the sterilization routine
• Downloadable performance protocol e.g., for internal and external audits including date, time, temperature reached, and a signature field
• Wizard provides short, clear, and illustrated step-by-step instructions, to ensure a standardized and complete procedure irrespective of different users
• Access to start sterilization can be restricted by user management

* Note for stacked devices: to avoid temperature control interference during 180 °C sterilization, we recommend running the process when the device directly above is idle. The device underneath will not be affected by the sterilization process.

Easy disinfection within a few minutes: Stainless steel chamber without bells and whistles
A key aspect of contamination prevention according to recommendations for Good Cell Culture Practice is frequent and complete wipe disinfection of the chamber interior – including the space behind cover sheets. For example, for CO₂ incubators it is recommended to do this monthly, typically followed by a 180 °C overnight sterilization. So, the more chamber parts and crevices you have in your CO₂ incubator shaker, the more time you need to invest for reliable contamination prevention. Especially if heating fins and cables covered by metal sheets are involved, this can be very time-consuming. In contrast, not cleaning and going for a "hope for the best"-approach bears a significant economic risk, especially if a contamination goes undetected over time.

The design of the CellXpert family including the CS220 follows the "If it's easy to do, it’s more likely to get done"-approach and lets you do proper cleaning within a few minutes:

• Seamless, stainless steel chamber
• Hygienic design according to EN ISO 14159:2008 standard: Fulfilled
• Only two parts (platform and sub-platform) can be removed easily within 1:30 min for full access to all chamber walls – no cover sheets that hide more
• Glide-up door glides up and out of your way so you can easily reach every corner of the device – even at the back of the chamber
• No drive wheel, crevices, cables, cover sheets, heating fins etc. inside for contaminants to hide behind

Save money and reach sustainability goals
• High flask capacity ensures lower maintenance per throughput than most other CO₂ incubator shakers in the market
• Highest platform size vs. footprint ratio in the market saves valuable lab space
• Only optional with most other devices in the market, but always included in the CellXpert CS220 product package: Slide-out platform, rH-control, touch interface, access port, sterilization routine
• No UV-lamps or open water bath plastic inlays that require frequent exchange
• Low gas and energy consumption

Glide-up door and slide-out platform: Saves space and ensures fast and easy experiment setup, cleaning and service - even at the back of the chamber
The space-saving door glides up and out of your way to ensure easy vessel loading onto the slide-out platform. The platform is locked and unlocked simply by the platform handle. In addition, the slide-out subplatform can be removed easily for full access and cleaning of the chamber bottom.

For optimized device uptime: Proven and tested Eppendorf X-drive
Unexpected downtime and late results are not an option for you? And do you have more important things to do than organize repair of your CO₂ incubator shaker over the weekend? The Eppendorf X-drive with four weight-supporting shafts and imbalance detection has been proven and tested in the market since years. It was designed to perform 24/7 at high loads with the Eppendorf reliability you are used to, so you can reach your goals in time and with peace of mind. Drive and drive wheel are positioned in a service drawer that opens to the front for easy access. This makes cleaning even easier and avoids the need to move the stack to have your drive checked.

Smart cell cultivation and easy documentation – with VisioNize^{^®}
• Optimize your daily work and increase your research safety using smart devices and connectivity options:
• 7” VisioNize touch interface shows all setpoints and current parameters at a glance
• Step-by-step on-screen wizard for 180 °C sterilization
• Filter and export performance charts, events, or performance protocols for 180 °C sterilization within seconds via VisioNize^® Lab Suite or the front USB-port
• Pre-installed and customer-programmed reminders e.g., for performance of a sterilization cycle, wipe disinfection, or performance checks with external sensors
• Integrated alarm system to define critical values e.g., door-opening time or gas concentration that trigger a highly visible on screen and audible alarm
• Download operation records to document cultivation conditions of individual experiments
• Set permissions and restrictions with on-board VisioNize user management
• Remote device monitoring and alert notifications with VisioNize Lab Suite

Care for your shaker – Service options
Biological shakers are fundamental to cell culture applications and must be able to run continuously for several years. Relatively small, short-term deviations in the shaker operation can have a significant impact on cultivation results, which can negatively affect the yield and/or cell expression. Eppendorf's Shaker Performance Plans ensure consistent, reliable shaker performance in accordance with manufacturer specifications. Shaker Performance Plans include a selection of preventive maintenance protocols to test and validate stable incubator performance.

Our installation qualification (IQ) and operational qualification (OQ) certification services support the requirements of your quality management and give you reliable confirmation that your biological shaker is working correctly and in accordance with the manufacturer's specifications.

Be confident about your cell culture conditions: Lab monitoring, asset management & instrument alert notification with VisioNize^{^®} Lab Suite
With VisioNize Lab Suite, Eppendorf offers you a cloud-based platform that easily connects, manages, and monitors your lab devices from anywhere. Track all related device parameters such as temperature, CO₂, rH, rpm as well as remaining program runtime. It also sends alert notifications via e-mail or SMS in case of anomalies.

With only one system you can manage recurring tasks such as performance of sterilization cycles, cleaning routines, or maintenance for your entire fleet centrally in one system. VisioNize Lab Suite has all the services to help you build a smart laboratory with increased sample safety, lab productivity and reliability.

Gain free, hands-on experience by connecting 3 devices for free.

Learn more about the VisioNize Lab Suite and its services

Eppendorf Bioprocess and harvesting solutions

Are you ready for scale-up or looking for a solution to harvest your cultivation products?
When the time comes to scale-up, you can expand your process with Eppendorf Bioprocess solutions – we are here to bring the tools and expertise required. We offer small scale to pilot/production scale bioprocess systems, ranging from 60 mL small-scale to 2,400 L fitting your specific application. Application and service support is there to assist you and your work – from first inquiry through final production. To harvest your large batches, Eppendorf provides matching sample separation solutions. Especially our high-speed centrifuges can help you to save handling time and efficiently handle volumes of 4 x 1.5 L or more.

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사양	CellXpert^® CS220	CellXpert^® CS220

Capacity	224 L (7.9 ft³)	224 L (7.9 ft³)
Power supply	220 – 240 V ±10 %, 50 – 60 Hz	220 – 240 V ±10 %, 50 – 60 Hz
Dimensions (W × D × H)	131.8 × 80.5 × 66.1 cm / 51.9 × 31.7 × 26.0 in	131.8 × 80.5 × 66.1 cm / 51.9 × 31.7 × 26.0 in
Product weight	240 kg / 529 lb	240 kg / 529 lb
High temperature disinfection (HTD)	180 °C	180 °C
Sealed inner glass doors	no	no
Segmented door options	–	–
Temperature control increment	0.1 °C	0.1 °C
Temperature stability at 37 °C	(at 22 °C ambient) ±0.1 °C	(at 22 °C ambient) ±0.1 °C
Temperature range	Ambient +8 °C to 60 °C	Ambient +8 °C to 60 °C
Audible and visual alarms	yes	yes
Temperature uniformity	±0.3 °C at 37 °C	±0.3 °C at 37 °C
CO₂ range	0.1 % – 20 %	0.1 % – 20 %
CO₂ control increment	0.1 %	0.1 %
CO₂ stability at 5 % CO₂	±0.1 %	±0.1 %
Drive mechanism	Eppendorf X-Drive	Eppendorf X-Drive
CO₂ uniformity	±0.1 %	±0.1 %
Number of shelves (standard/max)	–	–
Data export interface	USB, Ethernet, VisioNize^®	USB, Ethernet, VisioNize^®
Benchtop (B), Under Bench (U), Floor (F) or Stackable (S)	F, S (x3)	F, S (x3)
Access port	1	1
On-board data logging	yes	yes
BMS relay	yes	yes
Platform size (cm/in)	51.4 × 93 cm (20.2 × 36.6 in)	51.4 × 93 cm (20.2 × 36.6 in)
Refrigerated	no	no
Slide-out platform	yes	yes
Door opening	glide up	glide up
Speed range	20 – 300 rpm with 2.54 cm (1 in) orbit, 20 – 250 rpm with 5.1 cm (2 in) orbit	20 – 300 rpm with 2.54 cm (1 in) orbit, 20 – 250 rpm with 5.1 cm (2 in) orbit
Capacity	extra large	extra large
CO₂ accuracy	±0.3% at 5% CO₂	±0.3 % at 5 % CO₂
CO₂ sensor type	NDIR	NDIR
Relative humidity range	Up to 85 %	Up to 85 %
Relative humidity sensor type	bidirectional control	bidirectional control
Relative humidity accuracy	±5 %	±5 %
Relative humidity sensor type	Capacitive sensor	Capacitive sensor
Relative humidity control increment	1 %	1 %
Relative humidity stability	±2 %	±2 %
Remote Device Monitoring and Notifications (VisioNize^®)	VisioNize^® touch enabled	VisioNize^® touch enabled
Temperature range	8 °C above ambient to 60 °C	8 °C above ambient to 60 °C
Temperature accuracy	±0.4 °C at 37 °C	±0.4 °C at 37 °C

Agitation
Orbit	2.5 cm (1 in)	5.1 cm (2 in)
Speed	20 – 300 rpm	20 – 250 rpm
Control increments	1 rpm	1 rpm
Control accuracy	±1 rpm	±1 rpm

다운로드: CellXpert^® CS220

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Accelerate Your Growth

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CellXpert® CS220 stainless steel chamber

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CellXpert® CS220 integrated 180 °C sterilization routine

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CellXpert® CS220 wipe disinfection

CellXpert® CS220 saves costs

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CellXpert® CS220 accessory compatibility

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CellXpert® CS220 real-time remote monitoring, alerts and more

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다운로드: CellXpert® CS220

CellXpert^® CS220 - CO₂ Incubator Shaker

다운로드: CellXpert^® CS220