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Bioprocessing Scale Up
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Moving to a bioreactor
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Bioprocess scale up strategies
An established strategy when transitioning to larger working volumes is to monitor and control a single parameter. However, keeping one process parameter constant will change others; therefore, the cells’ growth environment can only be partly reproduced at different scales. Bioengineers must identify the parameters which influence cell growth and productivity most, to decide, which scale up strategy is the most suitable. Although bioprocessing scale up strategies that control several criteria are the most powerful, those that control singular parameters can still be effective, and are historically the most widely used due to their simplicity. Common singular parameters to control include:
Constant power input per liquid volume
- Np: the impeller power number (also known as Newton number),
- ρ: DI water density (kg/m3 ),
- N: agitation speed (rps),
- D: impeller outer diameter (m)
- V: full working volume (m3 ).
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Constant tip speed
Definition: The impeller tip speed equals π x d x N, with
- D: impeller outer diameter (m)
- N: agitation speed (rps)
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Constant kLa
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Constant mixing time
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Scale-up strategies: recommended resources
- Watch our webinar to find out more about bioprocess scale up strategies and how we scaled-up a cell culture bioprocess from 3 L to 40 L.
- Read in our application note , how we investigated scale up-relevant engineering parameters for differently sized fermentors, including power numbers, tip speed range, and oxygen transfer capabilities.
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Which factors are relevant during bioprocessing scale up?
As depicted above, a tailored scale up strategy is needed to reproduce product yield and quality in an upstream bioprocess across scales. Various other factors need to be considered as well while scaling-up, including space requirements in the lab, staff training, equipment choice, and scaling-up the downstream process. In this panel discussion, industry experts from Eppendorf and other companies share their thoughts on scale up challenges from an economical, operational, and application-related point of view.
Eppendorf products for powerful bioprocessing scale up
- Small-scale: DASbox® Mini Bioreactor System and DASGIP® Parallel Bioreactor Systems
- Bench-scale: SciVario® twin , BioFlo® 120 , BioFlo® 320
- Pilot/production-scale: BioFlo® 510 , CelliGen 510 , BioFlo® 610 , BioFlo® Pro , BioFlo® 720
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Cell culture scale up
- Single-use bioreactors: The BioBLU® c Single-Use Bioreactor portfolio covers working volumes from 100 mL to 40 L. To make the progression from bench-scale to production-scale, the BioFlo® 720 is a bioreactor control system which is compatible with Thermo Scientific™ HyPerforma™ 5:1 Single-Use Bioreactors with working volumes up to 2000 L.
- Glass bioreactors: Eppendorf glass bioreactors for cell culture applications cover working volumes from 60 mL to 10.5 L and are compatible with the small and bench scale Eppendorf bioprocess systems .
- Sterilize in place systems: The CelliGen 510 bioreactor control system covers working volumes from 10.5 L to 32 L.
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Fermentation scale up
- Glass bioreactors: Eppendorf glass bioreactors for microbial applications cover working volumes from 60 mL to 10.5 L and are compatible with the small and bench-scale Eppendorf bioprocess systems .
- Sterilize in place systems: The BioFlo® 510 , BioFlo® 610 and BioFlo® Pro cover working volumes from 16 L to 2400 L.
- Single-use bioreactors: The BioBLU® f Single-Use Bioreactor portfolio covers working volumes from 65 mL to 3.75 L. These stirred-tank bioreactors fully address the specific needs of fermentation. Read more about the scalability of E. coli fermentation in BioBLU® f Single-Use Bioreactors.
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Scalable portfolio
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How our customers use our products
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