Next-Generation Sequencing (NGS) has revolutionized the ‘omics’ era with the rapid decoding of massive DNA sequences, supporting the investigation into various biological processes in human disease, genetic inheritance, immunity, cancer, and others.
Generating high-quality NGS libraries is a labor-intensive process that requires experience, precision, and accuracy. Optimal, thorough sample preparation upstream of the sequencing process is essential to ensure the best possible results.
Join the experts at Eppendorf for this webinar to get practical advice on getting started with NGS in the lab, and discover how you can bypass pipetting-intensive protocols and automate your sample preparation with Eppendorf epMotion®—saving you time and effort and help you achieve reliable and reproducible sequencing results.
In this webinar, you will learn about:
Speakers:
Centrifugation is more than just spinning — it’s an essential part of your day-to-day lab life. A centrifuge is a device, generally driven by an electric motor, that spins a container, typically a rotor, at very high speed around a fixed axis, generating a centrifugal force. This ubiquitous method is used to separate the various components of a fluid by applying a centrifugal force — and each application requires a different speed, volume or vessel type. It's, therefore, crucial to grasp the principles of centrifugation to prepare for your next centrifugation experiment.
Eppendorf introduced its first centrifuge in 1964, revolutionizing life science research practices worldwide. With a range of centrifuges — from Minispin and bench-top, to floor-standing and ultracentrifuges — the Eppendorf name remains synonymous with creative design, innovative technology, and reliable performance that stand the test of time.
Whether you’re a young scientist who’s just starting out in the lab, a seasoned researcher who wants a refresher, or someone just interested in centrifugation, this webinar will benefit you. We’ll share our practical knowledge and expertise, and highlight all the essentials you need to know for conducting successful centrifugation experiments.
In this webinar, you will learn about:
Date & Time: 17th of March 2022
Speakers:
“The Bio Revolution” fosters innovations which transform not only our economies, but our societies, and all our lives [1]. At the same time digitization sparked a fourth industrial revolution, which is based on the rapid exponential development of new digital technologies [2]. Consumer-centric digital ecosystems are emerging across the world in response to these fundamental forces. Those two tendencies are not mutual to one another, but effect and reinforce each other. This results in a faster convergence between research & development, scalable production, and digital services. You as a scientist are an important parts of the new evolving ecosystem.
Join our upcoming webinar and learn how you can enhance your lab environment and bridge the gap between R&D and production by means of digital solutions. Eppendorf provides an integrated portfolio comprising software, instruments, consumables, and services, which can satisfy the demands of your research. Since monitoring and control is key in some fields, e.g. bioprocess development to ensure the process environment for optimum cell growth and high-titer production, we provide in-depth data and information regarding the influencing process parameters, to develop economical and process related KPIs.
[1] McKinsey Global Institute: The Bio Revolution.
Online click here.
[2] Kagermann, H.; Anderl, R.; Gausemeier, J.; Schuh, G.; Wahlster, W.: Industrie 4.0 im globalen Kontext: Strategien der Zusammenarbeit mit internationalen Partnern. Hrsg.: acatech - Deutsche Akademie der, T. Herbert Utz Verlag, München, 2016.
Date & Time: January, 25th at 9:00 AM & 5PM CET
Speaker:
Simon Wieninger, Business Manager Digital Solutions
Bio Speaker:
Creating digital solutions to empower scientist and biotech companies to make the most of
their lab work. This is the core goal Simon pursues as the Business Manager Digital Solutions at Eppendorf. Having a background in strategic consultancy within different industries, he always focuses on the value created for customers. Simon received his Ph.D. from RWTH Aachen University, with the center his research being on Strategic Management, Business Transformation and Business Ecoystems.
The last 18 months have shown us how important digital solutions have become to enable collaboration and to work remotely. With less access to the lab and your colleagues, the move to digital tools is even more important.
This webinar will give an introduction to where the digital lab space can become a reality for you and how to begin your digital transformation. We will cover the areas where digitization will improve your workflow and sample safety, giving you more time to focus on what is important.
Target groups:
Date & Time: 2nd of November 2021 | 9 am & 5 pm CEST
Speaker:
The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to data reliability. Despite choosing the appropriate assay type there are many more factors to be considered to mitigate data variability. This joint Promega and Eppendorf webinar series gives a comprehensive overview of the cell culture workflow and introduce measures to maximize the outcome of your cell-based assay.
In this episode, our experts will review the most frequently asked questions, that have been collected over the past years, including:
Join us to participate in the live Q&A session. If you can´t make it just register to receive the link to the recorded session.
Date & Time: October 7, 2021 at 7am PST, 10am EST, 4pm CET
Speakers:
In biologics production, the upstream bioprocess contributes significantly to product quality and manufacturing efficiency. A comprehensive understanding on which process parameters are critical and how they influence process outcome allows the development of tailored control strategies and, as a result, achieving consistent process performance.
Compared to offline measurements in a sample that has been taken from the bioreactor, inline analysis of process parameters reduces the manual workload and facilitates the implementation of automated feedback control loops. While inline analytics of some process parameters, such as temperature, DO, and pH is well established, real-time measurement of others, such as metabolite concentrations or cell viability is not yet routine.
Here we present strategies for in line process analytics that aimed at automated feed control in an upstream bioprocess. Using the example of a Raman analyzer, a glucose analyzer, and an exhaust analyzer we demonstrate, how to achieve seamless sensor integration with the bioprocess control software. In all cases we met the challenge of keeping the glucose concentration stable during the bioprocess run and minimize human intervention.
Key Learning Objectives:
Duration (optional): 35 minutes
Date & Time: Webinar took place on September 29, 2021
Speaker:
A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost with an 'auto-pilot' mindset. However, how many secrets are hidden inside a simple device? What differentiates between different models in the market?
These secrets can bring your lab productivity to the next level without major changes in cost or practices. Understanding your thermal cyclers and peripheral accessories will guide you in your next purchase, experimental planning, as well as improve overall PCR results.
Join live to be part of the live Q&A session
Scheduling conflict?
If you can´t make it, just register to receive the link to the recorded session.
In this webinar you will learn:
Date & Time: Webinar took place on September 14, 2021
Speakers:
With the advent of new diseases and infections, antibodies are becoming a powerful tool in fighting diverse clinical settings: oncology, chronic inflammatory diseases, transplantation, infectious diseases and cardiovascular medicine.
Antibody drug discovery involves more cutting-edge techniques for the research and development. It is slightly more complex than a typical drug development, primarily due to methodology like target assessment & validation, screening preparation, hit generation, screening & lead selection, lead optimization & characterization, candidate selection and further scale up in bioreactor.
Eppendorf portfolio uses the instruments which not only support specific aim but also support entire workflow, thus working as a complete bundled solution for the antibody production. Use of these packages from Eppendorf ensures reproducibility, lower lead times and complete hassle-free joy of ownership.
This webinar covers various aspects of antibody research and aims at reducing total time for the discovery.
Date & Time: Webinar took place on Thursday, July 15, 2021
Speakers:
MSC production is an expensive and high cost process with a high price to pay for any failure in MSC production. Conventional culture of Mesenchymal stem cells (MSC) either in the lab or cGMP facility is mostly in an in the environment with 21% oxygen level. However, stem cells in human tissues live in an environment with much lower oxygen concentration (<5%).
In this webinar, our guest speaker- Dr. Chua Kien Hui will talk about the benefit of culture MSC in hypoxic conditions include an increase in proliferation rate, enhanced paracrine growth factors secretion, better angiogenesis properties as well as with very low risk of tumorigenesis. He will also delve into other tips and best practices that a skilful stem cell production scientist should have to make sure the risk of contamination and cell growth is in the optimum stage.
Date & Time: Webinar took place on Wednesday, July 14, 2021 at 10am SGT
Speaker:
Successful bioprocessing is complex and depends on many factors. From the selection of the expression host, media composition, the proper cultivation system and cultivation strategy, adequate downstream processes, up to the development of a robust tech transfer.
In this webinar we will address general and day-to-day challenges in the bioprocessing workflow from shake flask to production and discuss possible solutions. We will initially look on common challenges in reactor set-ups and configurations. What vessel and impeller type are the appropriate one for different culture demands (e.g. shear sensitivity, high oxygen demands), what process parameters control is essential and how the integration of additional analytical tools can help to improve process stability. We will address frequent challenges in cultivation, e.g. how to produce reproducible inoculums in shake flasks, how to prevent contamination in a bioreactor, during transfer or sampling and how to cope with difficulties in cultivation in the bioreactor, e.g. foaming or viscous solutions. We will further have a look on the different steps of a process and discuss optimization factors and measures.
Register now, if you want to have answers to frequently asked questions in bioprocessing and want to understand how to cope with challenges in bioprocessing from shake flask to bioreactor.
If you want to get answers on specific questions during the webinar, send an email to bioprocess-experts@eppendorf.com by June 6.
Key Learning Objectives:
Duration (optional): 1 hour
Date & Time: Wednesday, June 23, 2021 at 9am EST| 2pm BST| 3pm CEST
Speakers:
El mercado global de biosimilares está en un punto clave pues en 2020 más de 10 de las principales bio-farmacéuticas concluyeron patentes en 2020. Muchos de esos fármacos son anticuerpos monoclonales humanos o humanizados. El objetivo del presente webinar es mostrar los resultados de tres principales métodos de producción para un biosimilar típico (lote, lote alimentado y perfusión) y su ejecución fácil y sencilla en un sistema de bioprocesos de mesa, lo que permite tomar decisiones confiables sobre el proceso para que sea escalable a producción.
Se comparan dos procesos de perfusión y para los cultivos en lote alimentado y en perfusión se mostrarán datos del uso de bioreactores de un solo uso con diseño de tanque agitado. Además de mostrar los datos de estos procesos, se proveerá de un análisis de los beneficios del uso del uso de estos sistemas en la producción de biosimilares con un enfoque particular en la mejora de tiempo y costo-beneficio.
Date & Time: May 14th, 2021
Speaker: Dr. Fernando Bautista Reyes, Product Specialist – Eppendorf do Brasil Ltda.
Spanish Language Webinar
En el laboratorio, las centrífugas están en todas partes, y son utilizadas con un objetivo en mente, la separación de mezclas al aplicar la fuerza centrífuga. Pero la centrifugación es más que solo hacer girar las muestras. Al igual que un auto en nuestro día a día, las centrífugas son herramientas útiles en el laboratorio de las cuales no podemos prescindir. Y al igual que un auto, hay algunos puntos a considerar para prolongar la vida útil del equipo.
En este webinar se hablará de los aspectos de seguridad más importantes en el uso de las centrífugas, así como de técnicas de mantenimiento para asegurar que estos caballitos de batalla del laboratorio permanezcan en condiciones óptimas.
Los objetivos del webinar son:
Date & Time: May 27th, 2021
Speaker: Dr. Fernando Bautista Reyes, Product Specialist – Eppendorf do Brasil Ltda.
Spanish Language Webinar
Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal changes in cultivation parameters can affect the viability, growth and metabolism of the cells. In this educative webinar we will discuss topics like Mycoplasma test methods, effects of passage numbers, and the usage of FBS. Join us to learn about the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture Practice into your daily routine.
Join live to be part of the live Q&A session
Scheduling conflict?
If you can´t make it, just register to receive the link to the recorded session.
In this webinar you will learn:
This webinar took place: May 6, 2021 at 7am PDT, 10am EDT, 4pm CEST
Speakers: Dr. Jessica Wagener (Moderation: Dr. Christian Haberlandt)
Shakers and bioreactors are established versatile systems for cultivating microorganism, plant, and animal cells in suspension. Both methods aim at increasing the oxygen and nutrient supply in the medium to stimulate culture growth, but offer very different solutions in technique, volumes/throughput, control possibilities, and thus applications.
In this educational webinar, we will look at the biophysical background of both cultivation techniques, pros and cons, and areas of application. You will also learn how to optimize your process in shake flasks and bioreactors via proper choice of vessel type, vessel material, instrument configuration, and parameter settings. Additionally, we will introduce the use of feeding techniques bioreactors and have a glance on shakers too.
Target groups:
Date & Time: Webinar took place on Wednesday, April 28, 2021
Learning Objectives:
Transferring a biologic candidate from the research and development phase to commercial production usually requires increasing the working volume of the upstream bioprocess. During scale-up, process performance optimized at small scale needs to be reproduced at larger scales, ideally without much need for process optimization at large working volumes. This requires reproducing the quality of the seed culture and the cells’ growth environment across scales.
To streamline scale-up, bioengineers commonly use bioreactors with similar geometries at all scales and keep one or more parameters constant between vessels of different sizes, such as kLa, power input per volume or tip speed. Therefore, to design a scale-up strategy, data on scale-up-relevant engineering parameters need to be available for each bioreactor planned to be used. These parameters include vessel geometries, power numbers, and oxygen transfer capabilities.
In this webinar we showcase scale-up of an antibody production process in CHO cells from bench to pilot/production scale. We discuss strategies to prepare seed cultures of reproducible quality in shake flasks. We demonstrate a new concept for software-aided scale-up strategy design, which considers vessel parameters of differently sized Eppendorf BioBLU® Single-Use Vessels and Thermo™ HyPerforma™ Single-Use Bioreactors.
Key Learning Objectives
Target group
This webinar is part of a virtual event. By attending the live webinar you will also have access to the Eppendorf Planet of Knowledge Platform on which more engaging and educational content about bioprocess scale-up will be provided.
Date & Time: The webinar took place January 14, 2021 at 10am EST| 3pm BST| 4pm CEST
Speakers:
An appropriate dissolved oxygen (DO) concentration in the culture medium is a key driver for high bioprocess yields. DO is determined by the oxygen supply to the bioprocess system and the oxygen consumption of the cultured microorganism simultaneously. In high-density fermentation of aerobic bacteria and fungi, for example, a large amount of oxygen must be introduced into the medium to avoid oxygen limitation during robust microbial growth. In other applications, like the cultivation of microaerophilic bacteria for probiotics production or gut microbiome research, the DO concentration must be controlled precisely at a low level.
In this webinar, we showcase advanced strategies to control the dissolved oxygen concentration in the culture medium and to reproduce it at different scales.
Key Learning Objectives:
Date & Time
The webinar took place December 08, 2020 at 9am EST| 2pm BST| 3pm CEST
Speaker
Ying Yang, Senior Research Scientist, Bioprocess, Eppendorf Inc.
Microinyección CRISPR - Opciones y consideraciones clave para crear modelos animales transgénicos
En este Webinar se abordarán los principios de la tecnología CRISPR, algunas de las opciones experimentales para su implementación en el laboratorio, así como sugerencias prácticas sobre el equipamiento necesario para la realización de una adecuada microinyección que permita la inyección exitosa de reactivos CRIPSR en modelos demandantes con alta reproducibilidad y sobrevivencia.
Date & Time:
19/November, 2020
Speaker:
Dr. Fernando Bautista Reyes, Product Specialist – Eppendorf do Brasil Ltda.
Spanish Language Webinar
Consejos prácticos para un mejor pipeteo y el cuidado de sus pipetas
En esta presentacíon se abordan los conceptos de precisión y exactitud de pipeteo de diversos tipos de líquidos en el laboratorio y algunos problemas: líquidos volátiles, líquidos viscosos, temperaturas diferentes en el ambiente y la pipeta.
Eppendorf mostrará como pipetar esos líquidos considerados difíciles con precisión y exactitud excelentes. Además de conceptos de limpieza de la pipeta, autoclavado y consejos de manipulación correta del instrumento.
• Precisión y exactitud;
• Pipetear líquidos considerados difíciles;
• Conceptos de limpieza de la pipeta.
Date & Time:
05/November, 2020
Speaker:
Adriana Elvia Mata Villegas, Sales Director LATAM-North & Central – Eppendorf do Brasil Ltda.
Spanish Language Webinar
Melhore a reprodutibilidade dos seus experimentos - Como técnicas de pipetagem e calibração podem influenciar positivamente seus resultados
Você sabe como é trabalhoso refazer rapidamente um experimento feito por seu colega ou um experimento sobre o qual leu no último artigo? Ela vai ou não vai funcionar em seu laboratório? Uma das atividades mais difíceis em um laboratório é garantir que os ensaios forneçam resultados comparáveis. Isso vale tanto para laboratórios diferentes quanto para pessoas diferentes dentro de um mesmo laboratório; e ao escalar experimentos para um maior rendimento.
A importância do manuseio de líquidos preciso é frequentemente subestimada ou não levada em consideração. Este webinar explicará como a transferência de líquidos realmente é crucial para garantir uma manipulação de líquidos confiável.
Exploraremos alguns pontos, a fim de tornar a transferência de líquido mais eficiente e, assim, economizar tempo. Serão abordados são as opções corretas de manuseio de líquidos para a transferência de líquidos em um ensaio mostrando como se trabalha com líquidos desafiantes: volátil e viscoso, qual a importância da calibração e manutenção se de seus instrumentos, além de dicas de quais instrumentos adequados para cada aplicação no laboratório.
- Opções corretas de manuseio de líquidos desafiantes: volátil e viscoso
- Importância da calibração e manutenção se de seus instrumentos
- Dicas de quais instrumentos adequados para cada aplicação no laboratório
Date & Time:
22/October, 2020
Speaker:
Adriana Freire Machado Msc., Head of Marketing Latin America, Eppendorf do Brasil Ltda. Msc in Infectious Diseases
Portuguese Language Webinar
Mejore su reproducibilidad - El manejo de líquidos es un factor crucial, en técnicas como NGS, ELISA y qPCR.
Requiere repetir un experimento realizado por algún colega o que encontró en la bibliografía. ¿Obtendrá resultados similares a los obtenidos por su colega o a los reportados? ¿Saldrá el experimento?
En la ciencia actual es crucial que cada laboratorio se asegure que sus resultados sean comparables con los de otros laboratorios que realizan experimentos similares. Es más, se ha vuelto un requerimiento para publicar en revista con un arbitraje alto. Esto es cierto tanto para diferentes laboratorios como para diferentes personas dentro de un mismo laboratorio, así como en el escalado de experimentos para una mayor productividad. La transferencia exitosa de experimentos toma tiempo, dinero y depende de muchos factores. Junto con el dicho “En tanto sea reproducible, la exactitud no es tan importante”, la importancia de la exactitud en el manejo de líquidos es muchas veces subestimada o simplemente no es tomada en cuenta.
Este webinar explica qué tan crucial es realmente la transferencia de líquidos para asegurar una transferencia de experimentos que sea confiable. Se muestran los puntos críticos en la preparación de librerías NGS, ELISA y qPCR para hacer la transferencia de experimentos más eficiente y por lo tanto ahorrar tiempo.
Date & Time:
08/October, 2020
Speaker:
Dr. Fernando Bautista Reyes, Product Specialist – Eppendorf do Brasil Ltda.
Spanish Language Webinar
Resolviendo los desafíos clave en cultivo celular.
Conforme los modelos experimentales animales están siendo sustituidos por modelos celulares, es necesario conocer todos los puntos críticos que ayudan a que estas células se desarrollen adecuadamente in vitro. Conocemos que requieren de un ambiente particular en el cual desarrollarse, crecer y llevar a cabo sus procesos. El cultivo in vitro se ve influenciado por temperatura y atmósfera principalmente, debiendo evitar la temida contaminación. En este webinar se abordarán las principales problemáticas asociadas con el control de estos parámetros y las soluciones que Eppendorf ha diseñado para resolverlas, así como los puntos clave para evaluar un sistema de incubación de CO2, asegurando reproducibilidad y confiabilidad en los experimentos realizados.
Date & Time:
27/August, 2020
Speaker:
Dr. Fernando Bautista Reyes, Product Specialist – Eppendorf do Brasil Ltda.
Spanish Language Webinar
Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of production often involves shake flasks and bioreactors. Shake flasks are used for bioreactor inoculum production and are an easy-to-use instrument for early process development like organism screening and media design. At the next process steps, highly controllable bioreactors are needed for flexible solutions, automated process control, and predictable scalability when larger quantities of cells are required. The challenge during upscaling is to keep a constant efficiency and quality during all process steps.
In this webinar, we will depict the typical applications of shakers and bioreactors in an upscaling bioprocess. We will highlight applicational differences and factors that influence cultivation and system transition. Furthermore, we will introduce to you the basic components of a bioreactor and the various process parameters that can be controlled. Finally, we will go through different process strategies to further optimize a process and increase production.
Key Learning Objectives:
Date & Time
This webinar took place December 2, 2020
Speakers
Ines Hartmann, Application Specialist Cell Handling, Eppendorf AG
David Solbach, Scientific Communications Manager, Eppendorf Bioprocess Center
When it comes to automation many think it increases productivity in the lab by simply increasing their sample throughput. But besides this obvious direct influence, the true power of automation for your lab goes way beyond a simple increase in numbers per run. Here you learn how Automation can also support your lab.
Date & Time:
Webinar took place on November, 19, 2020.
Speaker:
Tim Schommartz, Ph.D., Global Marketing Manager, Automation and PCR, Eppendorf AG
Vaccination is the most effective medical intervention ever introduced to eliminate large part of the infectious diseases. The development of a right vaccine has its own share of challenges and difficulties in getting into the market. Of any vaccine candidate development, the main flagbearer is Upstream process on which the success of the product largely depends. Further optimization of this upstream process requires critical analysis of numerous parameters which is possible only with highly evolved and improving bioprocess systems apart from scientific acumen. This presentation mainly focuses on the steps involved in the Vaccine development and provides a one stop shop solution for vaccine research and development thru Eppendorf Bioprocess Systems.
Key Learning Objectives:
Date & Time:
Webinar took place on Wednesday, November 11, 2020 at 8am CEST| 11.30am IST| 2pm SGT
Speakers:
Ananth Sharan, Infield Application Specialist, Bioprocess, Eppendorf India Ltd.
The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology.
Reproducibility of cell-based assays is one of the key points when it comes to data reliability. Despite choosing the appropriate assay type there are many more factors to be considered to mitigate data variability. This joint Promega and Eppendorf webinar series gives a comprehensive overview of the cell culture workflow and introduce measures to maximize the outcome of your cell-based assay.
In this episode, our experts will review the most frequently asked questions, that have been collected over the past years.
Join us to participate in the live Q&A session. If you can´t make it just register to receive the link to the recorded session.
Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous workflows have been successfully automated for a wide variety of applications in genomics, cell biology, and drug discovery. So why is there hesitation to move forward with automation?
Evaluating the many options for automated liquid handling can be overwhelming. When considering an automation platform for your lab, it is important to understand the level of flexibility, customization, or standardization needed.
Join live for a Q&A session with experts!
Scheduling conflict?
If you can´t make it, just register to receive the link to the recorded session.
In this webinar you will learn:
Date & Time:
November 10, 2020 at 7:00 am PST, 10:00 am EST, 4:00 pm CEST
Speakers:
Paulina Kocjan, Regional Marketing Manager for automation, PCR, and digital solutions, Eppendorf North America, Inc.
Kirk Mantione, Ph.D., Field Application Specialist, Eppendorf North America, Inc.
Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large number of proteins, lipids, messenger, and micro-RNAs. It was shown that they play a role in the mediation of intercellular communication, the modulation of immune-regulatory processes, tumor metabolism, and regenerative as well as degenerative processes. In recent years, there is increasing interest in the therapeutic potential of exosomes produced by Mesenchymal Stem Cells (MSCs).
Stirred-tank bioreactors offer the possibility to tightly control and monitor the production of exosomes, as well as the scalability, to produce increasing amounts. However, the cultivation of stem cells in stirred-tank bioreactors requires profound knowledge and precise control of the process.
In our talk, we will highlight the potential and benefits of stirred-tank bioreactors in the cultivation of stem cells for exosome production. We will share our knowledge on how we developed the process in our DASbox® Mini Parallel Bioreactor System and scaled-up the process to 1 L controlled by our newest parallel bioreactor control system, the SciVario® twin.
Key Learning Objectives:
Who Should Attend:
Date & Time:
Thursday, November 5, 2020 at 10am EDT | 9am CDT| 3pm BST | 4pm CEST
Duration: 1 hour
Speakers:
Jorge Escobar, Senior Research Scientist in the Applications Lab of Eppendorf, Inc.
Aurélie Tacheny, Project Manager and Application Specialist for Eppendorf Application Technologies
Matraces Erlenmeyers, placas de Petri y frascos de cultivo son las primeras palabras que vienen a la mente cuando pensamos en el cultivo de células eucariotas y procariotas en el laboratorio. Los biorreactores y fermentadores abren nuevas posibilidades y son una alternativa a considerar cuando requerimos de mayores cantidades de células, más eficiencia y alta productividad en nuestros procesos.
En este webinar discutiremos las diferencias entre el cultivo de células en agitadores y biorreactores:
Date & Time:
September 24, 2020
Speaker:
Marcelo Aguiar Msc., Zone Sales Director LATAM de Bioprocesos, Eppendorf do Brasil Ltda.
How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about how to pipette right. With this educational webinar we would like you to stop for a moment, re-think what you are doing, and significantly improve your skills and results.
We would like to share Eppendorf´s almost 60 years of experience in proper liquid handling with you – for more reproducible cell culture results and better contamination protection in your lab.
Join us to participate in the live Q&A session. If you can´t make it just register to receive the link to the recorded session.
Learning Objectives:
Date & Time:
October 8, 2020 at 7:00am PDT, 10:00am EDT, 4:00pm CEST
Speakers:
Nadine Mellies, PhD, Application Specialist Manual Liquid Handling, Eppendorf AG
Jessica Wagener, PhD, Application Specialist Cell Handling, Eppendorf AG
Shakers and bioreactors are established versatile systems for cultivating microorganism, plant, and animal cells in suspension. Both methods aim at increasing the oxygen and nutrient supply in the medium to stimulate culture growth, but offer very different solutions in technique, volumes/throughput, control possibilities, and thus applications.
In this educational webinar, we will look at the biophysical background of both cultivation techniques, pros and cons, and areas of application. You will also learn how to optimize your process in shake flasks and bioreactors via proper choice of vessel type, vessel material, instrument configuration, and parameter settings. Additionally, we will introduce the use of feeding techniques bioreactors and have a glance on shakers too.
Target groups:
Learning Objectives:
Date & Time:
September 22, 2020 at 7am PDT, 10am EDT, 4pm CEST
Speakers:
Ines Hartmann, Application Specialist Cell Handling, Eppendorf AG
Ankita Desai, Bioprocess Field Application Specialist, Eppendorf North America, Inc.
Shake flasks, cell culture dishes, and T-flasks are the first vessels that come to mind when we think about cultivation systems for growing eukaryotic and prokaryotic cells in the lab. Bioreactors and fermenters open up new possibilities and are another alternative to consider if we need larger quantities of cells, increased efficiency of cultivation, or enhanced reproducibility.
Within this webinar, the participants will learn the differences between the cultivation of cells in shakers and bioreactors. We will focus on the basic components of a bioreactor and the various process parameters that can be controlled. Finally, we will focus on different process strategies to further optimize a process and increase.
Key Learning Objectives:
Who Should Attend:
Duration:
1 hour
Date & Time:
September 15 2020 at 7am PDT, 10am EDT, 4pm CEST
Speaker:
Joerg Schwinde, Key Segment Manager for Vaccines/ Monoclonal Antibodies, Eppendorf AG – Bioprocess Center, Juelich, Germany
Vaccines Production - Bioprocess development and scale up
Nesses tempos excepcionais e sem precedentes, muitas empresas estão trabalhando ansiosamente para o desenvolvimento de uma vacina para conter o COVID-19. Novas tecnologias de bioprocessos, como equipamentos de uso único e automação de processos, abrem novas possibilidades para o controle e validação de qualidade. Isso é especialmente importante em ambientes regulamentados por GMP, como no desenvolvimento e fabricação de vacinas de influência. Ao enfrentar um surto de pandemia, a necessidade de desenvolver novos processos e aumentar rapidamente o volume de produção clínica é essencial para o desenvolvimento de novas vacinas com eficiência
Date & Time:
June 19, 2020
Speaker:
Marcelo Aguiar Msc., Zone Sales Director, Eppendorf do Brasil Ltda.
Portuguese Language Webinar
Vaccines Production - Bioprocess development and scale up
En estos tiempos excepcionales y sin precedente, muchas empresas están trabajando afanosamente para el desarrollo de una vacuna para contener el COVID-19. Las nuevas tecnologías de bioprocesos, como los equipos desechables y la automatización de procesos, abren nuevas posibilidades para el control y la validación de la calidad. Esto es especialmente importante en los ambientes regulados por GMP, como en el desarrollo y fabricación de vacunas de influenza. Al enfrentar un brote pandémico, la necesidad de desarrollar nuevo procesos y de aumentar rápidamente el volumen de producción clínica es esencial para el desarrollo de nuevas vacunas con eficiencia.
Date & Time:
June 19, 2020
Speaker:
Marcelo Aguiar Msc., Zone Sales Director, Eppendorf do Brasil Ltda.
Spanish Language Webinar
Automating RNA purification and PCR reactions
En el estudio de las enfermedades infecciosas emergentes, el aislamiento y la cuantificación de ácidos nucleicos es el punto de partida, tanto para el desarrollo de investigaciones clínicas como para el desarrollo de terapias y vacunas. Es en estas áreas de investigación donde los requerimientos de procesamiento de un alto número de muestras, la obtención de datos robustos y la generación de resultados reproducibles, determinan la aproximación metodológica y tecnológica a utilizar en el flujo de trabajo del laboratorio. Por ello en esta charla revisaremos los puntos críticos, los beneficios y los retos de los principales métodos empleados en el aislamiento y cuantificación de ácidos nucleicos; así como las soluciones que Eppendorf, en colaboración con diversos fabricantes de kits de purificación de ácidos nucleicos, ofrece a las personas involucradas en estas áreas de investigación e interesadas en automatizar sus procedimientos de manejo de líquidos.
Date & Time:
April 24, 2020
Speaker:
Fernando Bautista Reyes, Product Specialist – Eppendorf do Brasil Ltda.
Spanish Language Webinar
Learn about the most common problems with cryostorage and how to solve them using eLABInventory
In this webinar you will learn:
Date & Time: June 2020
Duration: 19 minutes
Speakers:
Stacey Willard
Learn more about biobank challenges in sample management and explore what advantages an Electronic Laboratory Notebook can offer you.
In this webinar you will learn:
Date & Time: June 2020
Duration: 15 minutes
Speakers:
Lara Matthews, Account Manager, Bio-ITech BV
Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vector production. To cultivate Vero cells in stirred-tank bioreactors, they require a growth matrix to attach to. The development of processes at bench scale is crucial to establish cost-efficient workflows in larger volumes. In this webinar, we demonstrate, how different process parameter can be monitored and controlled in a perfusion bioprocess to cultivate anchorage-dependent cells. We demonstrate the great potential of using packed-bed bioreactors or microcarrier spin filter for attachment cell-based vaccine production.
Date & Time: Tuesday, June 23, 2020 at 10am EST| 3pm BST| 4pm CEST
Duration (optional): 1 hour
Speakers:
Amanda Suttle
Research Scientist, Bioprocess, Eppendorf Inc.,
Exploring the gains and challenges of digitizing your laboratory data. Where to start and what to consider for your first steps in tomorrow's lab. A practical approach for you and your lab.
Covered fields:
Date & Time: June 11, 2020 at 10am CEST / 01:30pm IST
Speaker: Stephen Dey, Operational Marketing Team Leader Northern Europe, Eppendorf UK Limited
In this webinar you will learn:
Date & Time
June 9, 2020 at 3:00 pm CEST, 06:00 am PT, 09:00 am ET
Speaker
Michael W. Pfaffl, Ph.D., Professor of Molecular Physiology at the Technical University of Munich
Tim Schommartz, Ph.D., Global Marketing Manager, Automation and PCR, Eppendorf AG
Rafal Grzeskowiak, Ph.D., Field Application Specialist, Eppendorf AG
Laboratory safety: How to use as centrifuges in ideal conditions and guarantee good results
Nesse momento, muitas centrífugas estão sendo intensamente usadas em laboratórios de análises clínicas, microbiologia e biologia molecular. Com isso, novos desafios surgem, como a contaminação por aerossóis que podem ser liberados para o ambiente. Neste Webinar, vamos falar sobre como se prevenir dos aerossóis e também sobre alguns conceitos básicos de centrifugação como a diferença entre RPM x Força G, uso de rotores além de dicas e cuidados de limpeza. São informações valiosas, que podem ajudar você a prolongar a vida útil de seu equipamento e melhorar seus resultados.
Date & Time:
May 14, 2020
Speaker:
Adriana Freire Machado Msc., Head of Marketing Latin America, Eppendorf do Brasil Ltda. Msc in Infectious Diseases
Portuguese Language Webinar
Learning objectives:
Date & Time:
Webinar took place on May 13, 2020
Speakers:
Jessica Wagener, Ph.D., Application Specialist Cell Handling, Eppendorf AG
Nadine Mellies, PhD, Application Specialist Cell Handling, Eppendorf AG
Aged more than one century, nucleic acid purification is anything but ordinary. Nucleic Acid Purification, also termed Nucleic Acid Extraction, is the first step in most genomic workflows. High quality and pure DNA and RNA in sufficient amounts is vital for downstream processes used in many areas of the biological and medical sciences. But besides being a staple method in most molecular biology laboratories the understanding of the principle and knowledge about the details of this crucial technique is often surprisingly low. A good understanding about this background however can help tremendously to optimize your workflow and troubleshoot existing protocols.
In addition, there is a constantly increasing need in for high-throughput assays and nucleic acid analyses in areas like pathogen screening and cancer research up to pharmacogenomics, whether with genomic nucleic acids, viral RNA, mitochondrial DNA or plasmids. Nucleic acid purification with filter plates and magnetic beads are perfectly suited for automation. We show you how to automate your purification routines to increase both sample throughput and reproducibility. In this webinar Eppendorf and MACHEREY-NAGEL are joining forces to optimize your workflow.
In this webinar you will learn:
Date & Time:
Webinar took place on April 30, 2020.
Speakers:
Tim Schommartz, Ph.D., Global Marketing Manager, Automation and PCR, Eppendorf AG
Andreas von Bohl, Ph.D., Head of Bioanalysis Automation Group, Macherey-Nagel
Milan Dieris, Ph.D., Product Manager – Bioanalysis Department Macherey-Nagel
The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to reliability of experimental data. Despite choosing the appropriate assay type, there are many more factors to be considered to decrease data variability.
In this webinar Eppendorf and Promega are joining forces to optimize your assay results. We will have a look at the complete cell culture workflow and discuss measures you can take to get the best out of your cell-based assay. Furthermore, practical tips and tricks to avoid common pitfalls will be discussed with one of our most experienced Cell Culture Application Specialists.
Join live to be part of the live Q&A session
Scheduling conflict?
If you can´t make it, just register to receive the link to the recorded session.
Learning Objectives:
Date & Time:
April 15, 2020 at 4:00pm CET, 07:00am PT, 10:00am ET
Speakers:
Jessica Wagener, Ph.D., Application Specialist Cell Handling, Eppendorf AG
Erik Bonke, Ph.D., Application Specialist, Promega GmbH
Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experiment you read about in the latest paper? Will and will it not work out in your lab setup?
One of the most annoying activities in a laboratory is assuring that assays give comparable results. This holds true for either different laboratories or even for different people within one and the same lab; and when scaling up experiments for higher throughput. Reliable assay transfer safes time and money and is dependent on many factors. Along the lines of, “As long as it´s reproducible, the accuracy is not that important” the importance of accurate Liquid Handling is often underestimated or not taken into account.
This webinar explains how crucial transfer of liquids really is to ensure a reliable transfer of experiments. We will dig into some critical points of NGS library preparation, ELISA and qPCR in order to make assay transfer more efficient and thereby save time.
Learning Objectives:
Date & Time:
December 4, 2019 at 7:00 am PST, 10:00 am EST, 4:00 pm CET
Speakers:
Katja Baesler, Ph.D., Application Specialist Manual Liquid Handling, Eppendorf AG
Ulrike Gast, Ph.D., Field Applications Specialist Liquid Handling, Eppendorf AG
Alisa Gruschka, Application Specialist Manual Liquid Handling, Eppendorf AG
How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend to stop thinking about how to pipette correctly. With this educational webinar we would like you to stop for a moment, re-think what you are doing, and significantly improve your skills and results.
We would like to share Eppendorf´s almost 60 years of experience in proper liquid handling with you – for more reproducible cell culture results and better contamination protection in your lab.
Join us to participate in the live Q&A session. If you can´t make it, just register to receive the link to the recorded session after the webinar.
Learning Objectives:
Speakers:
Nadine Mellies, Ph.D., Application Specialist Cell Handling, Eppendorf AG
Katja Baesler, Ph.D., Application Specialist Manual Liquid Handling, Eppendorf AG
Stirred-tank bioreactors are gaining more and more importance in the industrial production of pharmaceutical products, biofuels and food. When compared with 2D-cultivation systems, bioreactors are easy to handle, and multiple bioreactors can be controlled in parallel without a considerable increase of workload. In stirred-tank bioreactors cells can be cultivated as free cells in solution, cell aggregates or on microcarriers in batch, fed-batch or continuous cultivation modes.
The first part of this webcast will feature a review of the basic principles of stirred-tank bioreactors. Ankita Desai of Eppendorf North America will explain factors that must be considered before and during the process, how to set up perfect conditions for cells, and how the shape of the impeller influences the stem-cell culture.
Within the second part, Arie Reijerkerk and Farbod Famili from Ncardia, a stem cell drug discovery and development company, will provide insight into how the advent of human-induced pluripotent stem cell (hiPSC) technology has substantially expanded the availability of human cells for drug discovery. The availability of these cells helps researchers to uncover the potential for better translation to the clinic and reduction of late-stage drug attrition.
Ncardia will present a case study of large-scale manufacturing development and their hiPSC-based drug screening platform, DiscoverHIT, including results from the generation of disease-relevant human stem cell-derived models and the development of predictive, phenotypic assays to enable high-throughput screening.
Date & Time:
Webinar took place on September 16, 2019
Duration (optional):
1 hour
Speakers:
Ankita Desai, Bioprocess Field Application Specialist, Eppendorf North America
Dr. Arie Reijerkerk, Head of Services and Innovation, Ncardia
Dr. Farbod Famili, Project leader assay development and high-throughput screening Ncardia
Key Learning Objectives:
Do you want to know how to increase the reproducibility of your cell-based assays and get practical advice on how to avoid common pitfalls? Don't miss our free new webinar including live Q&A session.
The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to reliability of experimental data. Despite choosing the appropriate assay type, there are many more factors to be considered to decrease data variability.
In this webinar Eppendorf and Promega are joining forces to optimize your assay results. We will have a look at the complete cell culture workflow and discuss measures you can take to get the best out of your cell-based assay. Furthermore, practical tips and tricks to avoid common pitfalls will be discussed with one of our most experienced Cell Culture Application Specialists.
Learning Objectives:
Date & Time:
Webinar took place on September 10, 2019
Speakers:
Jessica Wagener, Ph.D., Application Specialist Cell Handling, Eppendorf AG
Erik Bonke, Ph.D., Application Specialist, Promega GmbH
PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides its age of 36 years it is still a core component of numerous complex applications. The PCR is the workhorse method in virtually every molecular biology laboratory and the gold standard in countless analytical assays. Despite its broad usage, there are several factors that need to be considered to design a successful PCR experiment. Starting from more apparent things like temperature settings and DNA template quality to less known factors as pipetting techniques and used consumables, this webinar covers multiple ways to improve your PCR. In addition, we will also take a closer look at the special requirements of qPCR as probably the most commonly used variant of traditional PCR.
Learning Objectives
Date & Time:
Webinar took place on September 5, 2019
Speakers:
Tim Schommartz, Ph.D.
Application Specialist Liquid Handling, Eppendorf AG
Perfusion cell culture processes help saving lab space, increasing volumetric productivity, achieving more consistent product quality, and improving production of less stable proteins. Experimentation at small and bench scale is crucial for the cost-efficient development of bioprocesses, which then can be transferred to larger production volumes.
In this webinar we present cell retention technologies suitable for small and bench scale bioprocessing. We show a case study of an ATF-based CHO cell perfusion process at a working volume of 1 L.
Learning Objectives
Date & Time:
Webinar took place on May 20, 2019, 9 a.m. EDT, 2 p.m. GMT, 3 p.m. CEST
Speaker:
Amanda Suttle, Bioprocess Research Scientist, Eppendorf North America
Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either impossible or it poses several problems that significantly reduce accuracy, precision and processing speed. In this webinar, you learn about the basics of viscosity, how to measure it, about different types of liquids and what influence viscosity has on your pipetting results. You also learn how to prevent problems with viscous liquids by using different pipetting techniques and appropriate instruments. Get some helpful tips and tricks from one of our most experienced Liquid Handling Application Specialists and find out how you could even pipette honey.
Learning Objectives
Date & Time:
Webinar took place on June 25, 2019, 11:00 a.m. EDT, 5:00 p.m. CEST
Speaker:
Katja Baesler PhD, Application Specialist Manual Liquid Handling, Eppendorf AG
PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug discovery, and regenerative medicine. As stem cell cultures are very sensitive and respond to the smallest influence, stable culture conditions are crucial to maintain reliability and reproducibility of experimental data. In this educational webinar we will look at the different cultivation parameters, which can affect the viability and proliferation of PSCs during expansion. Join us to learn more about how to assess the quality of your stem cells and to preserve their pluripotency.
Key learning objectives:
Date & Time:
Webinar took place on June 18, 2019, 4:00 p.m. CEST
Speakers:
Jessica Wagener PhD, Application Specialist Cell Handling, Eppendorf AG
Nadine Mellies PhD, Application Specialist Cell Handling, Eppendorf AG
Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal changes in cultivation parameters can affect the viability, growth and metabolism of the cells. In this educative webinar we will discuss topics like Mycoplasma test methods, effects of passage numbers, and the usage of FBS.
Join us to learn about the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture Practice into your daily routine. Our most experienced Cell Culture Application Specialists will share their knowledge with you.
Learning Objectives
Date & Time:
Webinar took place on Wednesday, June 5, 2019, 8:00 a.m. PDT, 11:00 a.m. EDT, 5:00 p.m. CEST
Speaker:
Jessica Wagener PhD, Application Specialist Cell Handling, Eppendorf AG
The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has revealed virtually all environments, including the human body, are teeming with diverse microbial communities. While scientists study microbes largely in the context of pathogenicity, it is now evident that human microbiota contribute biological functions essential to health. Conversely, disrupting microbiota homeostasis in healthy individuals can lead to dysbiosis and is associated with many diseases and pathologies.
As a consequence, research into the human microbiome offers the potential to transform the healthcare landscape and provide novel diagnostics and therapeutics. Other aspects similarly impacted by metagenomics include biological safety of food and water. In agriculture, changes in soil and in animal microbiomes can now be evaluated in a context of crop yield, livestock health, and reduced dependency on herbicides, pesticides, and antibiotics. Transformative applications of microbiome sequencing in diagnostics, drug development, public health, agriculture, and water safety will be discussed.
Key learning objectives:
Date & Time:
Webinar took place on May 16, 2019, 4:00 p.m. CEST
Speakers:
Rita Colwell, PhD, University Professor at the University of Maryland at College Park and President of CosmosID, Inc
Christiane Honisch, PhD, Global Head Business Intelligence and Development, Eppendorf AG
PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug discovery, and regenerative medicine. As stem cell cultures are very sensitive and respond to the smallest influence, stable culture conditions are crucial to maintain reliability and reproducibility of experimental data. In this educational webinar we will look at the different cultivation parameters, which can affect the viability and proliferation of PSCs during expansion. Join us to learn more about how to assess the quality of your stem cells and to preserve their pluripotency.
Key learning objectives:
Date & Time:
Webinar took place on March 7, 2019, 8:00 a.m. and 4:00 p.m. CET
Speakers:
Jessica Wagener PhD, Application Specialist Cell Handling, Eppendorf AG
Nadine Mellies PhD, Application Specialist Cell Handling, Eppendorf AG
PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug discovery, and regenerative medicine. As stem cell cultures are very sensitive and respond to the smallest influence, stable culture conditions are crucial to maintain reliability and reproducibility of experimental data. In this educational webinar we will look at the different cultivation parameters, which can affect the viability and proliferation of PSCs during expansion. Join us to learn more about how to assess the quality of your stem cells and to preserve their pluripotency.
Key learning objectives:
Date & Time:
Webinar took place on December 4, 2018
Speakers:
Jessica Wagener PhD, Application Specialist Cell Handling, Eppendorf AG
Nadine Mellies PhD, Application Specialist Cell Handling, Eppendorf AG
Next-generation sequencing sample preparation is a labour-intensive process, which requires experience, precision and accuracy to generate high-quality NGS libraries. This webinar will enable you to understand the most common pain points with NGS library preparation and show you how to develop lab strategies that guarantee reproducible NGS results and increase overall lab productivity.
You will learn how to improve your NGS library preparation step-by-step and if switching from manual to automated solutions makes sense in your lab.
Learning Objectives:
Date & Time:
This webinar took place on Tuesday, November 13, 2018
Speakers:
Dr. Hanaë König, Global Marketing Manager, Automated Liquid Handling, Eppendorf AG
Marc-Manuel Hahn PhD, Application Specialist, Automated Liquid Handling, Eppendorf AG
The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to reliability of experimental data. Despite of choosing the appropriate assay type, there are many more factors to be considered to decrease data variability.
In this webinar Eppendorf and Promega are joining forces to optimize your assay results. We will have a look at the complete cell culture workflow and discuss measures you can take to get the best out of your cell-based assay. Furthermore, practical tips and tricks to avoid common pitfalls will be discussed with one of our most experienced Cell Culture Application Specialists.
In this webinar you will learn:
Date & Time:
This webinar took place on Tuesday, November 13, 2018
Speakers:
Jessica Wagener, PhD, Application Specialist Cell Handling, Eppendorf AG
Erik Bonke, PhD, Application Specialist, Promega GmbH
Sequencing of RNAs from extracellular fluids, including serum and plasma, is a fast-growing area of biomarker research. The need to process small quantities of samples is increasing as early detection strategies become more effective and less invasive biopsy strategies are adopted.
Moreover, the rapidly changing landscape of molecular testing points towards a need for minimizing sample input and preservation of sample for future testing.
This webinar provides insights into important factors that affect NGS library quality when using challenging samples such as cell-free Nucleic Acids (cfDNA and cfRNA). It also outlines strategies to overcome these negative effects as well as highlights automated liquid handling as an option to reduce human errors and increase productivity.
Learning Objectives:
Date & Time:
This webinar took place on Thursday 18th October 2018
Speaker:
Kanhav Khanna, Application Specialist, Automated Liquid Handling, Eppendorf North America
Are ethanol and antibiotics the best measures to prevent microbial contamination? Contamination is an everyday challenge in cell culture labs. Undetected or ignored contamination endanger the reliability and reproducibility of experimental data. Get to know the most effective methods to detect mycoplasma and to fight against the common types of contaminants. In this educational webinar you will learn how to recognize and identify different types of contaminants in cultivated cells. We will discuss how to apply aseptic techniques and preventive hygiene and give practical tips for preventing contamination by incorporating standard procedures into your daily culture practice.
Learning Objectives:
Date & Time:
This webinar took place on October 9, 2018
Speaker:
Jessica Wagener, PhD, Application Specialist Cell Handling, Eppendorf AG
Next-generation sequencing sample preparation is a labour-intensive process, which requires experience, precision, and accuracy to generate high-quality NGS libraries.
This webinar will enable you to understand the most common pain points with NGS library preparation and show you how to develop lab strategies that guarantee reproducible NGS results, and increase overall lab productivity.
By demonstrating protocols of NGS market leaders, including Illumina® and an introduction to the easy-to-use Eppendorf epMotion software – in an attempt to reduce the fear of liquid handling robots being difficult to use – you will learn how easy it is to implement ready to run automated NGS library prep protocols.
Learning Objectives:
Date & Time:
This webinar took place on September 20, 2018
Speakers:
Tim Schommartz, PHD; Application Specialist Liquid Handling, Eppendorf AG
Marc-Manuel Hahn; Applications Specialist Liquid Handling, Eppendorf AG
Three-dimensional (3D) cell aggregates are of great interest for many applications, including disease modeling, drug toxicity assessment, and manufacture of stem cell-based products. Stirred-tank bioreactors are promising culture systems for 3D cell aggregates, as they allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes. Furthermore, they can be operated in perfusion mode, which allows 3D cell aggregates to be sustained longer than in traditional batch cultures.
This webcast will review a research example for process development with the human tumor cell line H157, cultivated in stirred-tank mini bioreactors as 3D cell aggregates.
Learning Objectives:
Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either impossible or it poses several problems that significantly reduce accuracy, precision and processing speed.
In this Webinar, you learn about the basics of viscosity, how to measure it, about different types of liquids and what influence viscosity has on your pipetting results. You also learn how to prevent problems with viscous liquids by using different pipetting techniques and appropriate instruments. Get some helpful tips and tricks from one of our most experienced Liquid Handling Application Specialists and find out how you could even pipette honey.
This webinar took place on June 5, 2018
Speaker:
Hanae A. Henke, PhD, Content Editor, Eppendorf AG
The NGS library preparation process is labor-intensive, requiring experience and attention to detail over hours. Eppendorf consumables, such as our Eppendorf LoBind® plates, and our thermocyclers have aided many laboratories to achieve great results. To further help you excel and streamline your workflow, Eppendorf’s automation solution, the epMotion® 5075 NGS provides a perfect addition to other Eppendorf solutions. This easy-to-use liquid handler comes with a special software, accessory and consumable package for ease of use in your lab.
Key Topics:
Your advantage: Time-consuming optimizations and setups have already been done in collaboration with market leaders, including Illumina®. So, you are ready to start and can leave all the tedious pipetting and complicated handling to the robot. Of course, we are more than happy to share the expertise achieved in working with the manufacturers and customers alike in this webinar.
Key Learning Objectives:
Date & Time:
This webinar took place on April 26, 2018
Speakers:
Tim Schommartz, PHD; Application Specialist Liquid Handling, Eppendorf AG
Marc-Manuel Hahn; Applications Specialist Liquid Handling, Eppendorf AG
Next Generation Sequencing für Ihr Onkologielabor
Spielen Sie bereits mit dem Gedanken, diesen Umstieg in Ihrem Labor zu implementieren?
Eines ist sicher: Besonders als Testanbieter in der Onkologie ist die Hochdurchsatzsequenzierung kaum zu umgehen, angesichts der stetig wachsenden Anzahl an tumorrelevanten Gene und Varianten! Es geht nicht um das Ob, sondern eher darum, wann und vor allem wie dieser Umstieg vollzogen wird.
Datum & Uhrzeit:
Dieses Webinar fand am 08. Februar 2018 statt
Sprecher:
Hanna Jerome, Application Specialist Liquid Handling, Eppendorf AG.
Ole Menssing, Central Europe, Illumina Cambridge Ltd.
In this webinar, we present a solution for automated glucose monitoring and feeding during a bioprocess run. We integrated an autosampler and an analyzer with the bioprocess control and were able to completely automate glucose control. The design of the automation program is fully customizable, allowing the user to implement their own control strategy with custom nutrient target concentration, custom sampling frequencies, etc.
Join Dr. Stacey Willard, as she shares insights on streamlining bioprocess development efforts through automation.
Key Topics:
Key learning objectives:
Date & Time:
This webinar took place on Thursday, November 16, 2017
Speaker:
Dr. Stacey Willard, Senior Technical Applications Specialist, Eppendorf Inc.
Eukaryotic cell cultures respond to the smallest of influences. Apart from the risk of contamination, minimal changes in cultivation parameters can affect the viability, growth and metabolism of the cells. In this educational webinar we will discuss Mycoplasma test methods, the effects of passage numbers and, the usage of FBS.
Join us to learn about the measures you can take to improve the reproducibility of your experiments by incorporating good cell culture practice into your daily routine.
Key learning objectives:
Date & Time:
This webinar took place on Wednesday, November 8, 2017
Speaker:
Dr. Jessica Wagener, Application Specialist Cell Handling, Eppendorf AG
Pipetting is an every-day task in a cell culture lab. We pipette so often that we don´t actually think about it. This educational webinar gives an insight into how pipetting techniques can improve reproducibility of experiments and decrease the risk of contamination when working with eukaryotic cells. We will discuss the huge potential lying in pipetting techniques to increase the quality of your results.
Key Topics:
Date & Time:
This webinar took place on November 2, 2017
Speakers:
Dr. Jessica Wagener, Application Specialist Cell Handling, Eppendorf AG
Dr. Hanae A. Henke, Application Specialist Liquid Handling, Eppendorf AG
Have you considered how your lab will operate in five years’ time? Are you preparing for the lab of the future?
The connected home is already here, with multiple products interacting to create a smart household. Tracking of data and events, remote monitoring and automation are all available, creating efficiency in today’s busy life.
The same needs for data management, security and traceability, systems integration and device management exist in the laboratory today.
We’ll be discussing how to begin connecting your laboratory including challenges and considerations. This will include a snapshot of the latest device management software, electronic notebooks and smart equipment.
Participate in our Webinar to discover what is important for your lab today, the future is now reality!
Key Learning Topics:
Date & Time:
This webinar took place on Thursday, October 5, 2017
Speaker:
Stephen Dey, Marketing Director, Eppendorf UK
Contamination is an everyday challenge in cell culture labs. Undetected or ignored contamination and misidentification of cell lines endanger the reliability and reproducibility of experimental results.
In this educational seminar you will learn how to recognize and identify different types of contaminants in cultivated cells. We will discuss how to apply aseptic techniques and preventive hygiene and give practical tips for preventing contamination by incorporating standard procedures (e.g. for introducing new cell lines to the lab and testing for mycoplasma) into your daily culture practice.
Key Topics:
Date & Time:
This webinar took place on Wednesday, September 26, 2017
Speaker:
Dr. Jessica Wagener, Application Specialist Cell Handling, Eppendorf AG
Centrifugation is more than just spinning. Like a car in our day-to-day life, centrifuges are useful tools in the laboratory that we can no longer do without. And just like a car, there are a few points to consider in order to prolong the lifetime of your device.
In this webinar you will learn about the most important safety aspects while using a centrifuge, as well as proper maintenance techniques to make sure your laboratory workhorse remains in top condition.
Date & Time:
This webinar took place on Wednesday, September 20, 2017
Speaker:
Nicole Seeligmüller, Application Specialist Sample Handling, Eppendorf AG
Eppendorf is known for decades of premium quality liquid handling handheld devices – now get in touch with the epMotion® automated liquid handling systems and learn how easy you can automate your everyday pipetting tasks. The webinar is directed to scientists and lab technicians with no or limited experience in automated liquid handling.
We will discuss routine liquid handling tasks and illustrate how fast and effortless they can be transferred to our liquid handling workstations. The range of Eppendorf’s automated liquid handling products will be presented and we demonstrate how their flexibility and features help saving time and increase the reproducibility of your pipetting tasks.
Key Topics:
Date & Time:
This webinar took place on Wednesday, September 6, 2017
Speakers:
Dr. Tim Schommartz, Application Specialist Liquid Handling, Eppendorf AG
Dr. Carsten Buhlmann, International Product Manager Automation, Eppendorf AG
The first human cell line HeLa established in 1951 has entered cell culture labs worldwide. It took 20 years until the first data on cross-contamination of human cell lines with HeLa cells were published. Up to now cross-contamination and misidentification of various cell lines is an increasing issue in cell culture labs and is associated with dramatic consequences for research.
In this educative webinar, we will discuss causes and consequences of cell misidentification as well as different methods to check cell identity. Join us to learn more about preventing cell misidentification by incorporating Good Cell Culture Practice into your daily routine.
Key learning objectives:
Date & Time:
This webinar took place on Thursday, June 29, 2017
Speaker:
Nadine Mellies, PhD, Application Specialist, Eppendorf AG
Eukaryotic cell cultures respond to the smallest of influences. Apart from the risk of contamination, minimal changes in cultivation parameters can affect the viability, growth and metabolism of the cells. In this educational webinar we will discuss Mycoplasma test methods, the effects of passage numbers and, the usage of FBS.
Join us to learn about the measures you can take to improve the reproducibility of your experiments by incorporating good cell culture practice into your daily routine.
Key learning objectives:
Date & Time:
This webinar took place on Tuesday, May 30, 2017
Speaker:
Dr. Jessica Wagener, Application Specialist Cell Handling, Eppendorf AG
Imagine you heard from someone that you were surrounded by millions of microorganisms. At home, at work, in the gym, even in nature: Every day they are there. Sounds scary doesn’t it? These microorganisms that surround us are so called airborne biological particles or just bio-aerosols for short and are categorized under the general term aerosols.
Very common laboratory activities produce aerosols and are carried out without even thinking about aerosols, but around the world there are many documented cases of laboratory-acquired infections resulting from the production and inhalation of infectious aerosols. In the daily laboratory work, centrifugation is just one source of aerosols, and you must take adequate precautions when working with hazardous or infectious substances.
In this educational webinar, you will learn what aerosols are, where they occur and how to centrifuge hazardous/infectious samples in a safe way.
Date & Time:
This webinar took place on Tuesday, May 24, 2017
Speaker:
Nicole Seeligmüller, Application Specialist Sample Handling, Eppendorf AG
The only important ergonomic features of pipettes are light weight paired with low operating forces, right? And ergonomic working only means the avoidance of pain?
Basically this is correct, but good ergonomics includes much more. We see ergonomics as a whole concept based on cognitive, physical and organizational aspects, called the three spheres. We give insight into each sphere talking about the importance of body and mind supporting organization of the lab, the workflow and the user. You will understand the background and relevance of ergonomic approaches in everyday and lab life. Furthermore, you will learn about the best seating and standing position during lab work and how to prevent repetitive strain injury after long hours of pipetting.
This webinar can help you design your workplace according to your needs and take the stress and strain out of your daily lab routine.
Date & Time:
This webinar took place on Thursday, May 18, 2017
Speakers:
Dr. Ulrike Gast, Application Specialist Liquid Handling, Eppendorf AG
Dr. Hanae Henke, Application Specialist Liquid Handling, Eppendorf AG
Pipettes and other liquid handling instruments are widely used devices in all life sciences labs. The transfer of liquids in milliliter and microliter volumes is an essential technique in molecular and cell biology as well as biochemistry and microbiology labs in industry, academia and the healthcare sector.
The importance of accuracy and precision of your liquid handling instruments cannot be overestimated. The smallest inaccuracy may have a severe impact on the experiment and ruin your results. Nevertheless, most users are not aware of this issue and continue working with the affected instruments.
In this educational webinar you will learn why the calibration of liquid handling instruments is important, how calibration is performed in a professional service center and which instruments should be calibrated on a regular basis.
Date & Time:
This webinar took place on Tuesday, April 4, 2017
Speakers:
Dr. Ulrike Gast, Application Specialist Liquid Handling, Eppendorf AG
Dr. Florian Bundis, Global Product Manager, Eppendorf AG
Experimentation involving human stem cells (HSCs) is one of the fastest growing fields in research and development due, in large part, to its potential for revolutionizing human disease treatment. There are multiple HSC platforms being investigated, including human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs). Both have distinct advantages and disadvantages, but what they share is a bottleneck at the stage of scale-up, or the ability to produce enough cells for downstream R&D purposes. In this study, we discuss the successful growth of HSCs, both hMSC and hiPSC, in BioBLU® single-use stirred-tank bioreactors from the small (0.3 L) to bench (3 L) scale. In particular, we highlight the production of one billion hMSCs in a single bioreactor, and the culture of hiPSC at the small scale in parallel bioreactors.
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Date & Time:
This webinar took place on Tuesday, December 6, 2016
Speaker:
Stacey Willard, PhD | Senior Technical Application Specialist, Bioprocess | Eppendorf, Inc.
We looked below the waterline and got unexpected findings
When was the last time you were upset about non-reproducible results of your experiment? Even though you checked all variables including the pipetting system used in your experiment, you were unable to find the cause.
In many cases the assumption is made that the pipetting system, consisting of pipette and pipette tips, cannot be a source of error as long as the tip fits the pipette. However, even if this is the case, this does not speak to the precision and accuracy of the pipetting result. The international standard ISO 8655:2002 considers pipettes and pipette tips as a system. With good reason!
In this webinar we will show the results of our comprehensive study about tip quality in system with the pipette. At the end of this webinar you will have an overview of several influencing factors impairing your pipetting results when using pipettes and tips. We found that in the same way that only the tip of an iceberg can be seen above water level, a number of other problems with pipette tips rather stay unknown.
Date & Time:
This webinar took place on Tuesday, November 29, 2016
Speaker:
Dr. Ulrike Gast, Application Specialist Liquid Handling, Eppendorf AG
It has been a decade since Shinya Yamanaka and Kazutoshi Takahashi first reported the Nobel Prize winning discovery of induced pluripotent stem cells (iPSCs). Since then the technology has created new opportunities in understanding human development and has become an important tool in disease modeling and drug screening. One of the main challenges for new labs adopting iPS cells is the inefficiency of reprogramming methods. In this educational webinar, we will present a 2 step procedure for neuronal and cardiac differentiation along with tips to improve reprogramming efficiency.
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Date & Time:
This webinar took place on Thursday, November 17, 2016
Speaker:
Rick I Cohen, Ph.D, Director, Stem Cell Training Course, Rutgers University, Department of Biomedical Engineering, Piscataway, NJ - US
You know Eppendorf for decades of high quality liquid handling handheld devices – now get in touch with the epMotion® automated liquid handling systems and learn how easy you can automate your everyday pipetting tasks. The webinar is directed to scientists and lab technicians with no or limited experience in automated liquid handling. We will discuss routine liquid handling tasks and illustrate how fast and effortless they can be transferred to our liquid handling workstation. Eppendorf’s automated liquid handling products will be introduced and we demonstrate how its flexibility and features help saving time and getting better reproducibility of your pipetting tasks.
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Date & Time:
This webinar took place on Thursday, October 13, 2016
Speakers:
Dr. Ulrich Wilkening, Director Applications Liquid Handling, Eppendorf AG
Dr. Carsten Buhlmann, International Product Manager Automation, Eppendorf AG
The global market for biosimilars is expected to reach US$25 bn by 2020, when more than ten global blockbuster biopharmaceuticals, with sales of more than US$67 bn, will be off-patent. Many of these drugs are human or humanized monoclonal antibodies (hMAbs).
The objective of the research project presented in this webinar was to determine if the three primary methods of production for a typical biosimilar (batch, fed-batch, and perfusion) may be simply and easily executed using a single, benchtop bioprocess control system to allow confident process decisions that are scalable to full production. We compared two perfusion processes. For the fed-batch and perfusion cultivations, we used stirred-tank single-use bioreactors.
This webinar will discuss the outcome of these studies, and will provide an analysis of the benefits of their respective usage in biosimilar production with a particular focus on improving time and cost efficiency.
This webinar took place on Tuesday, June 14, 2016
Speaker:
Stacey Willard, PhD | Senior Technical Application Specialist, Bioprocess | Eppendorf, Inc.
Pipetting is an every-day task in a cell culture lab. We pipette so often that we don´t actually think about it. This educational webinar gives an insight into how pipetting techniques can improve reproducibility of experiments and decrease the risk of contamination when working with eukaryotic cells. We will discuss the huge potential lying in pipetting techniques to increase the quality of your results.
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This webinar took place on May 31, 2016
Speakers:
Dr. Jessica Wagener, Application Specialist Cell Handling, Eppendorf AG
Dr. Hanae A. Henke, Application Specialist Liquid Handling, Eppendorf AG
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Transferring liquids is part of almost every workflow in the life sciences. Some liquids like organic solvents, highly viscous or detergent-containing solutions pose special challenges to the experimenter. Pipetting them correctly requires awareness and special tools. Even everyday pipetting tasks such as PCR set-ups or changing cell culture medium have hidden dangers like stickiness of the reaction mix or foam formation of the medium. In this webinar we will highlight major liquid classes and their specific challenges. We will show typical examples for working with these liquids and present tips and tricks for handling them successfully. This webinar will help you achieve better results when working with problematic liquids.
This webinar took place on Tuesday, April 5, 2016
Speakers:
Dr. Hanae A. Henke, Application Specialist Liquid Handling, Eppendorf AG
Dr. Rudolf Walczak, Global Product Manager Cell Handling and Liquid Handling, Eppendorf AG
Photometry is a widely used analytical method in molecular biology labs. In many applications like cloning, sequencing, or PCR, you are working with nucleic acid preparations of various sources. Here, knowing the quality and quantity of your starting material is crucial to the reliability and reproducibility of your experimental results. But how do you ensure applying photometric methods correctly?
In this educational webinar, you will learn the basics of photometric nucleic acid determination and which critical factors affect the measurement. We will discuss the correct interpretation of the results and provide tips and tricks for troubleshooting.
This webinar took place on Wednesday, January 27, 2016.
There are many advantages to growing mammalian cells in suspension: adaptability, ease of handling, passaging, and scaling-up for bulk production and research applications. However, problems of cellular aggregation occurs more frequently in suspension culture and can adversely impact the health and productivity of your culture. In this educational webinar, we will present a novel method of developing aggregation-free HEK-293 cells.
Learning Objectives:
This webinar took place on November 5, 2015.
Bioprocessing is the study of living organisms used within the manufacturing process, such as the production of biofuels and pharmaceuticals. This webinar will discuss the role of single-use technology across a wide variety of processes, including shear sensitive cell cultures, robust microbial applications, and adherent cell line development.
Kevin Voll, Product Manager at Eppendorf, will discuss:
This webinar took place on October 19, 2015.
There are many advantages to growing mammalian cells in suspension: adaptability, ease of handling, passaging, and scaling-up for bulk production and research applications. However, problems of cellular aggregation occurs more frequently in suspension culture and can adversely impact the health and productivity of your culture. In this educational webinar, we will present a novel method of developing aggregation-free HEK-293 cells.
Learning Objectives:
This webinar took place on September 1, 2015.
Substances leaching out of plastic consumables – so-called “leachables” – are still frequently underestimated in laboratory workflows. While possible effects of leachables are routinely taken into account in pharmaceutical or food research, this topic is still underrepresented in the majority of life science applications. Recent scientific evidence shows however that leachables can significantly affect experiments and pose a likely source of error in many assay systems for example, by inhibiting enzyme activities in bioassays or falsifying nucleic acid quantification.
This educational webinar presented by Eppendorf and hosted by LabRoots provides a comprehensive overview of what leachables are and their role in laboratory workflows as well as gives recommendations on how to:
This webinar took place on June 3, 2015.
To harness the potential of human pluripotent stem cells (hPSCs), abundant provision of their differentiated progenies is required. During this webinar we present how hPSC expansion as matrix-independent aggregates in suspension culture (3D) was successfully combined with cardiomyogenic differentiation. A multi-well screening approach was scaled-up to stirred-tank bioreactors applying controlled feeding strategies (Batch and Cyclic Perfusion) followed by differentiation.
Optimized conditions in 100 mL cultivations enabled the production of 40 million cardiomyocytes (>80% purity), the majority of which displayed ventricular-like action potentials and were directly applicable for bioartificial cardiac tissue formation, a potential strategy for heart repair, drug discovery and safety pharmacology.
Learning Objectives:
This webinar took place on June 9, 2015.
