The optimized thawing program of the Eppendorf ThermoMixer C with the SmartBlock cryo thaw thaws 1-2 mL of cells frozen at -80 °C within 3-4 minutes with up to 100% cell viability with minimal shaking movements (500 rpm). The program is reproducible, is not subject to temperature fluctuations and is energetically favorable due to the overall speed. The risk of contamination is minimized as each tube thaws dry in its own well. The Eppendorf SmartBlock cryo thaw allows the use of up to 24 cryo vessels at the same time. In this way, several users can safely thaw their cells at the same time. In addition, the ThermoTop™
can be used to heat the samples from above to avoid condensation. Thus, the concentration of ingredients in the medium remains the same.
If necessary, the SmartBlock Cryo thaw can be cleaned very easily with disinfectant and after a few minutes it is ready for use again.
Medium can be transferred to 15, 25, or 50 mL conical tubes and heated in an appropriate thermoblock. This means that only the amount that is actually required is defrosted. The risk of contamination is also reduced here compared to using the entire bottle and less medium is disposed of.
In summary, it can be said that reproducible, clean thawing of medium and cells with excellent cell viability in a thermomixer is possible and recommended. It simplifies and accelerates everyday laboratory work.