Bioprocessing R&D

Microorganisms & Cells

Research and process development are key elements in the creation of improved, more rapid, and lower-cost methods for producing bio-based products. Working with small volumes saves valuable resources while industry-standard bioprocess systems deliver the precision required to operate under production-like conditions. Advanced software solutions feature real-time process control as well as comprehensive data and information management.  The Quality by Design (QbD) approach and statistic tools such as Design of Experiments (DoE) are effective means for a faster development . Single-use bioreactors further improve turn-around times and simplify validation.

»The DASGIP system enables an enormous experimental throughput. It is an important and reliable platform for metabolic engineering in explorative research at Evonik.«

Dr. Jörg Hennemann, Group Leader SciTech, Evonik Creavis GmbH

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Preculture

Preculture

Precultures are the link between storage and bioprocessing. The stackable Innova® 44/44R shakers and the New Brunswick S41i CO2 Incubator fulfill all demands of user-friendly and reliable shake-flask cultivation. Its triple-eccentric drive mechanism provides worry-free, 24/7 shaking.

  • Shakers
    • Stackable – up to three units for maximum space savings
    • Programmable Innova controls automatically changes temperature, speed and optional photosynthetic and UV germicidal lights at timed intervals
    • Versatile accessory platform (sold separately) accommodates flasks
      up to 5 L
    • Slide-out platform mechanism — provides easy and effortless access
      to flasks located in the front and back of the shaker/incubator
    • Built-in water reservoir humidifies chamber to reduce sample evaporation, while also protecting unit from spills, includes
      a drain for easy cleaning.
  • Pipette controllers
    • Intuitive and convenient speed adjustment made simple with the
      tips of your fingers
    • Lightweight, well-balanced and ergonomic design that allows
      for fatigue-free pipetting
    • Vibrant backlit LEDs provide optical feedback of the remaining
      battery life
    • Lithium polymer rechargeable battery offers long cordless runtime
    • Smooth setting of pump speed
  • CO2 Incubators
    • Temperature range from 4 °C above ambient to 50 °C
    • Shaking speeds between 25 – 400 rpm (±1 % full scale), 25 – 300 rpm
      if stacked, provides versatility for culturing a wide range of cell types.
    • Large 35.6 x 61 cm (14 in x 24 in) platform accommodates flasks
      up to 4 liters providing flexability with your cultures
    • Single or double stacked for incresed capacity
    • Inner and outer door saves valuable gasses while maintaining the perfect enviornment for your cells
  • Dishes, Flasks & Plates
    • Easy and fast well identification by contrast rich individual well ID and OptiTrack® alphanumeric labeling
    • Moat surrounding the outer wells to prevent the “edge effect” when
      filled with liquid
    • Chimney-well design to enable the filling of the inter well spaces of the complete plate to level out well-to-well temperature shifts outside
      the incubator
    • Robust stacking performance by pronounced rims on plate lid and excellent fitting of lid and base when used in stacks
    • Lid taps under the plate for minimized surface contact and therefore reduced risk of contamination
Cultivation & Analysis

Cultivation & Analysis

The DASbox Mini Bioreactor System is the superior tool for screening under production-like conditions. Additionally, the DASware software solutions enable seamless integration of sampling and analytical devices, and Design of Experiments. Various photometers complement our offerings.

  • Photometers
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetic
      (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM
      to 2,000 nM fluorescein with the BioSpectrometer fluorescence
  • Bioprocess Systems
    • Comprehensive and scalable hardware and software solutions for R&D, process development, pilot, and production in both cell culture and microbiology
    • Small scale and scale down models, parallel operated bioreactors, bench scale solutions, and sterilize-in-place systems
    • Covering working volumes of 60mL - 2,400L
  • Bioreactors
    • Rigid wall stirred-tank single-use vessels for microbiology and cell culture, covering working volumes from 65mL - 40L
    • Wide range of industry standard autoclavable glass bioreactors
    • Stainless steel sterilize-in-place vessels
  • Design of Experiments (DoE)
    • Built-in full factorial DoE: easy definition of experimental factors and responses; center points and randomized positioning of runs
    • Integration of 3rd party DoE tools like JMP and others
    • Recipe generator supporting multiple system layouts
  • Analyzer Integration
    • Integration of 3rd party lab devices into bioreactor control units
    • Enables bidirectional OPC interconnectivity, process-triggered feedback control loop and sampling on demand
    • Allows for online calculations and event- or data-driven decisions
    • Users benefit from integration of existing benchtop equipment, better process understanding and optimal process control
    • Integration includes nutrient analyzers & cell counters, biomass monitors, mass spectrometers, automation platforms and autosamplers, HPLC and Raman spectroscopy
Harvesting & Preparation

Harvesting & Preparation

The speed adjustment of our electronic pipettes helps to remove the cells very carefully and precise.
A variety of different consumables such as the well-known Safe-Lock Tubes or our Conical Tubes (15 mL and 50 mL) can be used for the preparation and/ or sample collection.

  • Centrifuges
    • Max. speed: 3,000 x g (4,400 rpm)
    • Very compact footprint to fit on every lab bench
    • Very quiet operation to benefit your environment
  • Pipettes and Tips
    • The ultralight Research was developed according to the strict criteria
      of the Eppendorf PhysioCare Concept.
    • Eppendorf PerfectPiston®: Ultralight and highly resistant piston system
    • The positions and shapes of the control buttons/displays have been optimized for stress-free work procedures and intuitive operation
    • The spring-loaded tip cone provides you with an optimal pipette fit with minimal insertion force (not available for 5 mL and 10 mL pipettes)
    • The adjustment option allows you to perfectly adapt the pipette for specific liquids or other external conditions.
  • Tubes & Plates
    • Hinged Eppendorf Safe-Lock lid prevents unintentional lid opening during incubation and storage
    • Polypropylene provides high resistance to chemicals, mechanical stress and temperature extremes
    • Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio-assay results
    • g-Safe®: Exceptional centrifugation stability up to 30,000 x g prevents sample loss due to tube breakage and provides extra safety when working with hazardous samples
    • Precise lid sealing for minimal evaporation rates during long-term storage
  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
Quantification & Evaluation

Quantification & Evaluation

DASware discover collects and archives bioprocess information like process values and events, recipes as well as analytical information. With the Eppendorf Detection equipment molecular analysis such as nucleic acid and protein quantification, kinetic studies, ELISA, fluorescence or microvolume measurements can be performed. Furthermore, the PlateReader AF2200 is equipped to analyze the status of your cells.

  • Photometers
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetic
      (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM
      to 2,000 nM fluorescein with the BioSpectrometer fluorescence
  • Cuvettes
    • Suitable for measuring small volumes (≥50 µL)
    • UV and Vis-transparent between 220 nm and 1,600 nm
    • Two built-in optical path lengths in a single cuvette-just turn the
      UVette 90° to change from 10 mm and 2 mm
    • Volume markings for 500 µL and 1,000 µL
    • Available in individually packaged PCR clean and protein-free quality
  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
  • Reader Plates
    • UV-VIS microplates feature an ultrathin film bottom for excellent light transmission in the UV range
    • Black Microplates offer an excellent signal-to-noise ratio - for clear signals even with low-concentration samples
    • White Eppendorf Microplates are optimized for highest sensitivity
      in the detection of luminescence signals by maximizing reflection
    • Solid black and white assay plates are made of polypropylene resulting
      in high resistance to chemicals, mechanical stress and temperature extremes
    • All plates are optimized for minimal autofluorescence and autoluminescence
  • Data- and Information Management
    • Easy analysis of bioprocess information using an intuitive web-based database query tool
    • Real-time retrieval of key process information · Batch-to-batch comparison of process information and trends
    • Tabulated and configurable views of all critical process information
    • Easy chart generation using the integral Chart Creator tool
    • Automatic Microsoft Excel and Adobe PDF process workbook generation
    • Supports cross platform comparison of runtime data between DASGIP and 3rd party systems
Storage

Storage

The New Brunswick freezers meet the highest standards for quality in regard to performance, sample security, and convenience. To concentrate your samples prior to the storage, the Concentrator plus is available as well as different Deepwell plates including sealing options.

  • Freezer
    • Provides 30% more storage capacity than freezers of equal size
    • Inner doors in upright models are gasketed and insulated creating three separate compartments minimizing cold air loss
    • Unique heated air vent with plunger to clear ice buildup and to relieve vacuum formation to gain immediate access to the freezer
    • Built-in voltage stabilizer on U535, U725, C585 and C760 freezers provides extra sample security
  • Deepwell Plates
    • OptiTrack® matrix: 30 % faster sample identification and fewer
      pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with
      heat sealing
    • Easy and reliable stacking
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
  • Sealing options
    • Storage Film and Foil glued seals for simple and dependable sealing during sample storage
    • Heat Sealing Film and foil for continuous locking with the best evaporation protection (see heat sealing materials)
    • Foil products are made of aluminum, can be pierced, and protect light-sensitive samples
    • Film products are transparent and provide protection against unwanted punctures
    • Eppendorf Plate Lid: stable, flexible protection of samples during short-term storage
  • Concentrator
    • Comes in 3 system configurations
    • Extremely quite operation <50 dB(A)
    • Chemical-resistant, maintenance-free PTFE diaphragm pump eliminates the need for changing up pump oil
    • Small footprint saves valuable bench space
    • Wide variety of 15 different rotor options to accommodate tubes from
      0.2 mL to 50 mL, microplates and PCR plates