Webinars

Vero Cell Cultivation for Vaccine Production

Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vector production. To cultivate Vero cells in stirred-tank bioreactors, they require a growth matrix to attach to. The development of processes at bench scale is crucial to establish cost-efficient workflows in larger volumes. In this webinar, we demonstrate, how different process parameter can be monitored and controlled in a perfusion bioprocess to cultivate anchorage-dependent cells. We demonstrate the great potential of using packed-bed bioreactors or microcarrier spin filter for attachment cell-based vaccine production.

Key Learning Objectives:

  •  Importance of high-quality pre-cultures for bioreactor inoculation
  •  Importance of process monitoring and control
  •  Hands-on expertise on how to cultivate and grow anchorage dependent cells on different attachment matrixes. 

Who Should Attend: 

  •  Experienced scientists looking for tips and tricks to improve their processes
  •  Scientists that are looking for solutions to produce enough material for the inoculation of large pilot and production sized bioreactors 
  •  Scientists who are interested in the cultivation of anchorage-dependent cells in stirred-tank bioreactors

Date & Time:
Thursday, October 22, 2020 at 10am EDT| 9am CDT| 3pm BST| 4pm CEST

Durtion:  1 hour

Speaker:
Michelet Dorceus, Bioprocess Field Application Manager, Eppendorf North America
 

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Michelet Dorceus, Bioprocess Field Application Manager, Eppendorf North America

Stem Cell Derived Exosome Production in Stirred-Tank Bioreactors

Shakers and bioreactors are established versatile systems for cultivating microorganism, plant, and animal cells in suspension. Both methods aim at increasing the oxygen and nutrient supply in the medium to stimulate culture growth, but offer very different solutions in technique, volumes/throughput, control possibilities, and thus applications. 

In this educational webinar, we will look at the biophysical background of both cultivation techniques, pros and cons, and areas of application. You will also learn how to optimize your process in shaker flasks and bioreactors via proper choice of vessel type, vessel material, instrument configuration, and parameter settings. Additionally, we will introduce the use of feeding techniques in shakers and bioreactors. 

Key Learning Objectives:

  •  Review: Why shake or stirr cells and an outline of system specific differences
  •  Learn how to identify the right cultivation method, vessel type, vessel material, and instrument configuration for your specific needs 
  •  Learn how to define and optimize important parameters to improve culture results – incl. Tips & Tricks  
  •  Learn about feeding strategies in shake flasks & bioreactorsand their impact on your culture
  •  Live “Ask the Expert” session – Deepen your knowledge and optimize your process

Who Should Attend: 

  •  Scientists new to cultivation of cells in suspension in shakers and/or bioreactors
  •  Experienced scientists looking for tips and tricks to improve their processes  

Date & Time:
Thursday, November 5, 2020 at 10am EDT | 9am CDT|  3pm BST | 4pm CEST

Duration: 1 hour

Speakers:
Jorge Escobar, Senior Research Scientist in the Applications Lab of Eppendorf, Inc.
Aurélie Tacheny, Project Manager and Application Specialist for Eppendorf Application Technologies
 

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Jorge Escobar, Senior Research Scientist in the Applications Lab of Eppendorf, Inc.
Aurélie Tacheny, Project Manager and Application Specialist for Eppendorf Application Technologies

Move Forward with Automation

Learn more about selecting an automation platform.

Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous workflows have been successfully automated for a wide variety of applications in genomics, cell biology, and drug discovery. So why is there hesitation to move forward with automation?

Evaluating the many options for automated liquid handling can be overwhelming. When considering an automation platform for your lab, it is important to understand the level of flexibility, customization, or standardization needed.
Join live for a Q&A session with experts!
 
Scheduling conflict? 
If you can´t make it, just register to receive the link to the recorded session.
 
In this webinar you will learn:

  •  How to evaluate automation and its benefits for your application
  •  Tips and tricks for automating DNA purification, NGS library prep, and PCR set up
  •  How to select the right liquid handling platform for your workflow

Date & Time:
November 10, 2020 at 7:00 am PST, 10:00 am EST, 4:00 pm CEST

Speakers:
Paulina Kocjan, Regional Marketing Manager for automation, PCR, and digital solutions, Eppendorf North America, Inc. 
Kirk Mantione, Ph.D., Field Application Specialist, Eppendorf North America, Inc. 


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Regional Marketing Manager for automation, PCR, and digital solutions, Eppendorf North America, Inc.
Ph.D., Field Application Specialist, Eppendorf North America, Inc.

Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis

The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to data reliability. Despite choosing the appropriate assay type there are many more factors to be considered to mitigate data variability. This joint Promega and Eppendorf webinar series gives a comprehensive overview of the cell culture workflow and introduce measures to maximize the outcome of your cell-based assay.

In this episode, our experts will review the most frequently asked questions, that have been collected over the past years. 

Join us to participate in the live Q&A session. If you can´t make it just register to receive the link to the recorded session.

Key Topics:

  • When to ideally time an analysis?
  • Does the passage number influence experimental outcomes?
  • When to choose what detection mode?
  • How to select the appropriate microtiter plate?
  • What is the best method for detecting mycoplasma?

Date & Time:
November 18, 2020 at 4:00pm CET, 10:00am EST, 07:00am PST

Speakers:
Jessica Wagener, Ph.D., Application Specialist Cell Handling, Eppendorf AG
Erik Bonke, Ph.D., Application Specialist, Promega GmbH
 

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Jessica Wagener, Ph.D
Erik Bonke, Ph.D