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Low-Throughput Screening Solution

Centrifuge Solution for screening assays and sequencing (spin down)

For the selection and characterization of antibody leads low-throughput screening assays are performed in microtiter plates (MTPs) various methods are required and all include multiple centrifugation steps. Centrifuge 5430 in combination with swing-bucket Rotor A-2-MTP offer an economical solution for two MTPs per run in a small footprint. Centrifuge 5430 also accommodates rotors for 15 mL and 50 mL conical tubes and high-capacity rotors for 1.5/2.0 mL tubes.

Increased Efficiency

  • Saves precious benchtop space: versatility of a multipurpose centrifuge with footprint of a microcentrifuge.
  • Rotary knob control for faster changes of speed and time.
  • Fast access to your most frequently used settings with five custom program buttons.

Improved Sample Safety

  • Automatic lid opening at the end of the run prevents sample warming and allows easy access to samples.
  • Newly inserted rotors are detected automatically to limit speed to the maximum permissible value and guarantee correct speed to g-force conversion.
  • For your safety, the device automatically detects imbalances during operation and stops the run immediately with an error message and a signal tone.

 

Better Ergonomics

  • There is no need to exert unnecessary force when closing the centrifuge lid. For ergonomic lid operation Eppendorf designed the soft-touch lid closure system. In combination with a low opening height you can close the centrifuge with ease.
  • Easy to read large display and intuitive operation.

 

Components

Centrifuge 5430
  • Crossover microcentrifuge with multipurpose versatility, non-refrigerated
  • Remarkable versatility with 12 different rotors
  • Max. capacity: 48 × 1.5/2.0 mL, 6 × 50 mL, 2 × MTP
Rotor A-2-MTP
  • 2-place swing-bucket rotor for MTP, PCR plates and DWP with windshield design for quiet operation
  • Max. capacity: 2 x plates
  • Max. speed: 2,204 × g (4,680 rpm)

Recommended Consumables for Key Methods

Recommended Consumables

Key Method Consumables Advantages

Sequencing (plate spin down)

 
  • Eppendorf twin.tec® PCR Plate 96, Skirted, PCR Clean, clear
  • DWP 96x500µl Protein LoBind®
  • Microplate 96 F-PP white/Clear, PCR-Clean
 
 
  • Minimization of rest volume and increased mixing & resuspension efficiency (even in 384 format) with optimized well geometry RecoverMax®
  • Minimization of unspecific sample loss with LoBind® consumables
 

Functional assays

 
  • Microplate 96 F-PP white/Clear, PCR-Clean
  • DWP 96x500µl Protein LoBind®
  • Twin.Tec PCR Plate 96, Skirted, PCR Clean, clear
 
 
  • Minimization of rest volume and increased mixing & resuspension efficiency (even in 384 format) with optimized well geometry RecoverMax®
  • Reduced intra- and inter-lot variance due to the sturdy construction of plates and the high lot-2-lot consistency
  • Minimization of unspecific sample loss with LoBind® consumables
 
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