What´s Next?
What´s Next?

NGS library preparation

Next Generation Sequencing

DNA sequencing is a method to decipher the base sequence in nucleic acids. The elucidation of the DNA sequence is essential for the understanding of virtually all biological processes e.g. human disease biology, inheritance, immunology, oncology, cellular biology. First generation sequencing devices use the capillary electrophoresis-based Sanger sequencing technique. The invention of the Next Generation Sequencing (NGS) technique massively improved the weaknesses of the 1st generation sequencing technique e.g. low throughput, scalability, speed, and resolution. NGS allows massive parallel sequencing which reduces the costs and increases the speed of DNA sequencing.

 

Automated NGS prep

Preparation & Purification

Preparation & Purification

Besides manual preparation and purification done in one of our centrifuges, automated systems for using magnetic beads or vacuum extraction are available. A broad range of further general laboratory instruments, consumables, pipettes and tips completes the options.

  • Centrifuges
    • Max. rotor capacity: 24 x 1.5/2.0 mL vessels
    • Max. speed: 20,238 × g (14,680 rpm)
    • OptiBowl® design for quiet operation, even without a rotor lid
    • Soft-touch one-finger lid closure for ergonomic operation
    • SOFT brake to protect sensitive samples
  • Pipettes & Tips
    • The ultralight Research plus was developed according to the strict criteria of the Eppendorf PhysioCare Concept.
    • Eppendorf PerfectPiston®: Ultralight and highly resistant piston system
    • The positions and shapes of the control buttons/displays have been optimized for stress-free work procedures and intuitive operation
    • The spring-loaded tip cone provides you with an optimal pipette fit with minimal insertion force (not available for 5 mL and 10 mL pipettes)
    • The adjustment option allows you to perfectly adapt the pipette for specific liquids or other external conditions.
  • LoRetention Tips
    • Ultrahomogenous surface for maximum reproducibility
    • Ultrahydrophobic surface for minimal sample loss
    • Significantly reduced foam formation during pipetting
    • Increase in reproducibility, e.g. for sensitive PCR and real-time PCR applications
    • Extreme resistance to chemicals
  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats
      from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
  • Automation
    • Various nucleic acid kits with Plug’n’Prep methods
    • Flexibility with open architecture & easy to use software
    • Magnetic bead or vacuum separation
    • Vacuum station fully integrated that automatically adapts
      to filter plates controlled by software
    • Gripper for plate & labware transport
Library Construction

Library Construction

For precise and accurate pipetting of your experimental set-up you can choose between manual pipettes or automated solutions. The incubation can be done at highly reliable conditions using our ThermoMixer with the ThermoTop lid.

  • Automation
    • Preprogrammed and optimized library prep method
    • Reliability with optical sensor checking labware, tips & liquid levels before the run
    • Use gripper transport, ThermoMixer, UV&HEPA and up to 15 deck positions
    • Automatic fixing of labware for mixing up to 2000 rpm
    • Pipetting at standard positions during shaking
  • Thermocycler
    • Extremely fast heating and cooling rates
    • Ultimate reduction of evaporation
    • Gradient blocks with SteadySlope® technology
    • Intuitive graphic programming
    • Display to indicate cycler number in a network
  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
  • LoBind Consumables
    • LoBind material guarantees maximum sample recovery for improved assay results
    • Free of surface coating (e.g., silicone) to minimize the risk of sample interference
    • Lot-tested and certified free of DNA, DNAase, RNase and PCR inhibitors (PCR clean)
    • Available in tube, microplate, and deepwell plate formats for easy-up scaling
    • Precise lid sealing for minimum evaporation rates
  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
Amplification / Enrichment

Amplification / Enrichment

Convenient and extremely fast PCR runs are guaranteed using our Mastercyclers, with or without gradient function for optimization. Using our LoRetention tips for pipetting ensures highest sample recovery.

  • LoBind Consumables
    • LoBind material guarantees maximum sample recovery for improved assay results
    • Free of surface coating (e.g., silicone) to minimize the risk of sample interference
    • Lot-tested and certified free of DNA, DNAase, RNase and PCR inhibitors (PCR clean)
    • Available in tube, microplate, and deepwell plate formats for easy-up scaling
    • Precise lid sealing for minimum evaporation rates
  • Pipettes & Tips
    • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid
      an accidential tip ejection a clear haptic feedback is provided.
    • The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
    • The Reference 2 multi channel offers a high flexibility including
      a spring-loaded tip cone which can be switched on/off optionally
    • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids
      or other external conditions without the need for a full calibration.
    • The quick lock enables an easy cleaning and maintenance of your pipette
  • LoRetention Tips
    • Ultrahomogenous surface for maximum reproducibility
    • Ultrahydrophobic surface for minimal sample loss
    • Significantly reduced foam formation during pipetting
    • Increase in reproducibility, e.g. for sensitive PCR and real-time PCR applications
    • Extreme resistance to chemicals
  • Thermocycler
    • Intuitive graphic programming
    • Small footprint
    • Universal block for ultimate flexibility
    • Connect up to 2 Mastercycler nexus eco to your existing unit
    • Available with gradient
  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
Analysis (Library QC)

Analysis (Library QC)

A system of Detection instruments and additional equipment allows you to select a combination that fits the required measuring performance. From UV/Vis and fluorescence applications to microvolume measurements: Anything is possible!

  • Photometers
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetic
      (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM
      to 2,000 nM fluorescein with the BioSpectrometer fluorescence
  • Cuvettes
    • Suitable for measuring small volumes (≥50 µL)
    • UV and Vis-transparent between 220 nm and 1,600 nm
    • Two built-in optical path lengths in a single cuvette-just turn the UVette 90° to change from 10 mm and 2 mm
    • Volume markings for 500 µL and 1,000 µL
    • Available in individually packaged PCR clean and protein-free quality
  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
  • Automation
    • 0.5 to 300 μL with one system
    • Electronic pipetting with synchronous 96 channel piston movement for better precision and reproducibility or results
    • Auto-detect function for tip size
    • Use 50 μL & 300 μL reload tips for best accuracy
    • Intuitive & industry proven software concept & convenient touch screen control
Sequencing

Sequencing

At Eppendorf, you can choose the optimal combination of products to prepare the sequencing set-up. Besides a variety of pipettes, tips, tubes and plates we are offering special LoBind consumables to ensure that nothing of your valuable sample gets lost at the material of the consumable itself.

  • Pipettes & Tips
    • The ultralight Research was developed according to the strict criteria
      of the Eppendorf PhysioCare Concept.
    • Eppendorf PerfectPiston®: Ultralight and highly resistant piston system
    • The positions and shapes of the control buttons/displays have been optimized for stress-free work procedures and intuitive operation
    • The spring-loaded tip cone provides you with an optimal pipette fit with minimal insertion force (not available for 5 mL and 10 mL pipettes)
    • The adjustment option allows you to perfectly adapt the pipette for specific liquids or other external conditions.
  • LoRetention Tips
    • Ultrahomogenous surface for maximum reproducibility
    • Ultrahydrophobic surface for minimal sample loss
    • Significantly reduced foam formation during pipetting
    • Increase in reproducibility, e.g. for sensitive PCR and real-time PCR applications
    • Extreme resistance to chemicals
  • Tubes & Plates
    • Unmatched, transparent polypropylene (PP) for improved sample visibility
    • OptiTrack® Matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • RecoverMax® well design: Optimized well geometry for minimal remaining volume and excellent mixing properties
    • Raised well rims and smooth surface for reliable closing, even with heat sealing
    • g-Safe®: exceptional centrifugation stability up to 6,000 x g
  • Deepwell Plates
    • OptiTrack® matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with heat sealing
    • Easy and reliable stacking