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Pipetting knowledge hub

PCR

Explore why PCR remains a cornerstone of DNA and RNA analysis in both research and diagnostics. Discover how this essential technique drives modern science and supports everyday decisions in the lab.

About the PCR workflow

PCR (Polymerase Chain Reaction) is a cornerstone technique for amplifying DNA used in virtually all molecular biology labs in R&D, diagnostics, and quality control. Regardless of running endpoint, quantitative, or even digital PCR experiments, the PCR run is preceded by the setup of master mixes and samples. With increasing sample numbers and conditions, this is becoming a tedious and error-prone task to conduct.

Challenges in PCR

Risk of cross-contamination leading to false positives/negatives
Need for precise reagent dispensing and mixing
High-throughput demands and time pressure
Ensuring reproducibility across plates and runs

How our automated liquid handlers help

epMotion excels in PCR setup in plate formats, including master mix distribution and sample addition, taking the pain out of this routine task. Our systems utilize filter tips, an enclosed deck, and liquid level detection to minimize contamination and errors. Automated workflows ensure that every well receives the correct volume, every time. Our CleanCap upgrade increases security by adding UV decontamination and air filtration. Full documentation for GxP compliance and traceability is yielded by our software.

Discover our epMotion systems

Why our expertise matters

We know that reliable PCR results require more than just good reagents: they require flawless liquid handling. Eppendorf’s experience in PCR, PCR plates, and automation means you benefit from proven protocols, expert support, and robust hardware that keeps your results consistent and your lab efficient.

Explore a wide variety of ready-to-use protocols for PCR setup and more. Discover how easy it is to own your solution.

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