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Centrifuge Solution for Plasmid construction, Sequencing preparation and Humanization

Target antigen production, sequencing sample preparation, and antibody humanization involve a great deal of repetitive molecular biology handling steps. This includes spin column based MiniPrep kits for nucleic acid purification, gel extraction, and PCR clean-up. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Microcentrifuges have entered a new generation, with the completely newly developed Centrifuge 5425 in combination with kit rotor specifically designed for 1.5/2.0 mL spin column kits.

Key benefits

INCREASED EFFICIENCY‌

  • Rotary knobs control for faster changes of speed and time.
  • Fast access to your most frequently used settings with three custom program buttons.
  • One push short-spin key for quick centrifugation – no need to hold key all the while.

IMPROVED SAMPLE SAFETY‌

BETTER ERGONOMICS‌

Components

Centrifuge 5425


  • Whisper quiet 24-place standard microcentrifuge with outstanding feature set for broad range of applications, non-refrigerated.
  • Extended rotor program (e.g. 5.0 mL rotor, swing-bucket rotor for PCR strips).
  • Max. capacity: 10 × 5.0 mL vessels.


Rotor FA-18x2-Kit

  • Fixed-angle rotor for molecular biology applications in 18 × 1.5/2.0 mL tubes or spin columns.
  • Aerosol-tight with Eppendorf QuickLock lid.
  • Max. speed: 18,565 × g (15,060 rpm).

Recommended Consumables

Key Methods

Consumables

Advantages

  • Plasmid Construction
  • Supernatant/Lysate clarification
  • Humanization (Plasmid construction, big volume)
  • Conical Tubes 50 mL rack (sterile)
  • Conical Tubes 25 mL, SnapTec® , Starter Pack, PCR clean
  • Safe handling of bigger volumes (25-50mL) due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap
  • Easy handling of bigger volumes (25-50mL) with SnapTec® lid
  • Minimization of DNA/RNA Sample degradation with PCR Clean purity grade
  • Minimization of unspecific sample loss with LoBind® consumables

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