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Protein analysis

Protein analysis spans immunoassays such as ELISA and Western blotting and mass spectrometry sample preparation, supporting high‑quality insights in discovery and translational research.

About the protein analysis workflow

Protein analysis workflows span two major types of experiments, quantitative immunoassays and mass spectrometry (MS) sample prep. The former comprises assays like ELISA, multiplex bead assays, and Western blotting, typical mass spectrometry workflows consists of protein extraction, reduction/alkylation, digestion, cleanup, and enrichment. These methods enable biomarker quantification, pharmacokinetic studies, and mechanism-of-action insights across discovery and translational research. Automation-ready formats, from 96/384-well plates for immunoassays to tube/plate-based proteomic prep, support scalable throughput and consistent data quality.

Challenges in protein analysis

Consistent pipetting of challeging liquid such as viscous buffers, detergents, and bead slurries
Minimizing cross-contamination and background signal
Ensuring precise liquid handling and timings
Preserving sample integrity and yield from low-abundance proteins

How our automated liquid handlers help

epMotion automizes protein workflows with precise liquid handling for ELISAs, bead-based assays, and MS sample prep. Flexible method without coding, gentle yet thorough mixing, and high labware compatibility simplify protocol design – from single-plex ELISA to high-throughput digestion and peptide cleanup. Barcoded labware tracking and standardized methods improve reproducibility and throughput.

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Why our expertise matters

Protein assays are sensitive to small deviations: pipetting accuracy, mixing efficiency, and timing directly impact signal and recovery. Eppendorf’s automation and liquid handling know-how helps safeguard assay linearity and digestion consistency, so your quantitation is reliable and comparable across runs. Our application support team assists with method setup and optimization for your kits and proteomics workflows, helping you accelerate from sample to insight.

Protein analysis spans immunoassays such as ELISA and Western blotting and mass spectrometry sample preparation, supporting high‑quality insights in discovery and translational research.

Explore a wide variety of ready-to-use protocols for protein analysis and more. Discover how easy it is to own your solution.

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