For which applications can the Eppendorf Vis Cuvettes be used?

All photometric detection methods with measuring wavelengths in the visible range of light could be applied, e.g. colorimetric protein detection, optical densities of bacterial cultures, enzyme kinetics.

What material are Eppendorf Vis Cuvettes are made of?

The Eppendorf Vis Cuvettes are made of PMMA

In which wavelength range of light are the Eppendorf Vis Cuvettes transparent?

The Eppendorf Vis Cuvettes are transparent for light in the visible range from 300 to 900 nm.

Are the Eppendorf Vis Cuvettes suitable for kinetic measurements?

If anexact temperature should be maintained during the measurement, we recommend to use the Eppendorf macro Vis cuvettes.

Is there a chemical resistance lists for the use of Eppendorf VIS Cuvettes?

Please check our information in the operating instructions in chapter 5.3. otherwise the chemical resistance of PMMA applies.

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Eppendorf Vis Cuvettes

Detailed picture of Eppendorf VIS Cuvette macro

Scientist working with Eppendorf Spectrophotometer

Product Information

The Eppendorf Vis Cuvettes are disposable cuvettes made of clear plastic with a light transmission of 300 nm to 900 nm. These cuvettes are perfect for applications outside of the UV range, such as colorimetric protein assays (Bradford, Lowry, etc.), determining of the optical density of bacterial cultures (OD600 methods), and kinetic and fluorescence measurements.

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Single Devices (2)

Accessories (3)

2 Single Devices

Eppendorf macro Vis Cuvette

plastic cuvette for measurements in the Vis range, max. filling volume 4,500 µL
1,000 cuvettes (10 boxes × 100 cuvettes)

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Catalog no. 0030079345

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Eppendorf semi-micro Vis Cuvette

plastic cuvette for measurements in the Vis range
max. filling volume 3,000 µL

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Catalog no. 0030079353

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Technical Data

Features

The Eppendorf Vis Cuvettes are disposable cuvettes made of clear plastic with a light transmission of 300 nm to 900 nm for spectrophotometers or photometers. The Vis Cuvettes are the perfect tool for applications outside of the UV range, for example, colorimetric protein assays (Bradford, Lowry, etc.), determining of the optical density of bacterial cultures (OD 600 methods), kinetic, and fluorescence measurements. Based on the volume you would like to measure, you can select semi-micro or macro cuvette.
Common photometric applications such as colorimetric protein assays (Bradford, Lowry, etc.), determining the optical density of bacterial cultures (OD 600) or kinetic measurements, often require a standard curve and many samples measurements. To support this high throughput, Vis Cuvettes are packaged in 10 boxes of 100 cuvettes each. The boxes enable safe storage of the cuvettes while offering convenient access to each cuvette. The Vis Cuvettes can be used in all common photometers that accommodate 12.5 mm x 12.5 mm cuvettes.
For safe handling, cuvettes should be handled in combination with specific cuvette holders.

Safe line-up of your cuvettes needed?

Due to their light weight and the small footprint, cuvettes tend to tip on the bench. To avoid this loss of high value samples due to these accidents, a dedicated rack for cuvettes is recommended. These racks have special square-footed wells for the cuvettes.
On top, the array of several cuvettes with individual sample content in a specific rack reduces the risk of mixing up your samples.

Video – Easy programming of standard curves for colorimetric assays with the Eppendorf BioSpectrometer

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Specifications	Macro Vis Cuvette	Semi-micro Vis Cuvette

Packaging	1,000 cuvettes (10 boxes × 100 cuvettes)	1,000 cuvettes (10 boxes × 100 cuvettes)
Dimensions (W × D × H)	12.5 × 12.5 × 45 mm	12.5 × 12.5 × 45 mm
Colorimetric protein assays	yes	yes
Fluorescence measurements	yes	yes
Light transmission	300 nm – 900 nm	300 nm – 900 nm
Maximum filling volume	−	−
Height of light source	all common cell heights	all common cell heights
Use in Eppendorf BioPhotometer^®	yes	yes
Use in Eppendorf BioSpectrometer^®	yes	yes
OD 600 methods	yes	yes
Temperature control	yes	no
Sample light path length (mm)	10 mm	10 mm
Cuvette blank	-	-
Material	PMMA	PMMA
dsDNA concentration range (UV 260 nm)	1 pg/µL 1,000 pg/µL (Pico Green Assay)	1 pg/µL 1,000 pg/µL (Pico Green Assay)
BSA concentration range (UV 280 nm)	0.01 ng/µL – 0.01 µg/µL (NanoOrange Assay)	0.01 ng/µL – 0.01 µg/µL (NanoOrange Assay)

Minimum filling volume
in Eppendorf photometers	−	−
in devices of other manufacturers	−	−