What is the Tube Clip used for?

The Eppendorf Tubes ® 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.

Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?

It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.

Is it necessary to buy new equipment to be able to use the new tube?

The bottom shape of the Eppendorf Tubes ® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion ® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265

What types of applications can be carried out with Eppendorf Tubes ® 5.0 mL?

Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur ® . They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.

Are the Eppendorf Tubes ® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?

The Eppendorf Tubes ® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur ® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.

Up to which g-force can the Eppendorf Tubes ® 5.0 mL be centrifuged?

All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.

How resistant are the Eppendorf Tubes ® 5.0 mL to chemicals?

The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.

Is it possible to store samples in 5.0 mL Eppendorf Tubes ® in the freezer?

Yes, the tubes can be stored down to -86 °C.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Tubes^® 5.0 mL - Microtube

Eppendorf Tubes_REG_ 5.0 mL placed in centrifuge rotor buckets

Eppendorf microtubes_REG_ 5 mL placed in reservoir rack module

Eppendorf microtube_REG_ 5 mL next to 1.5 mL microtube and 15 mL conical tube

Eppendorf microtubes_REG_ 5 mL in high speed rotor

Eppendorf microtubes_REG_ 5 mL in amber with closed lid

Eppendorf microtube_REG_ rack with 5 mL and 1.5 mL tubes.

Eppendorf Microtube_REG_ 5 mL inside conical bottom centrifuge adaptor

5 mL microtubes in the Eppendorf ThermoMixer_REG_ C

Scientist pipetting into Eppendorf 5 mL microtubes_REG_ stored in rack

Eppendorf Tubes_REG_ 5 mL with screw cap and snap cap open

5 mL microtubes - screw cap, snap cap and amber

The ultimate screw cap microtube for sample security.

Screw cap microtube 5 mL with Eppendorf stamp

Eppendorf Storage Box: Space for up to 5 x 5 Eppendorf Tubes_REG_ 5 mL

Tubes 5.0 mL screw cap, amber and transparent

Light Protection as well as Transparency

Selection of Eppendorf tubes_REG_ including microtubes in 0.5 mL, 1.5 mL, 5 mL format, and conical tubes in 15 mL and 50 mL format

Product Information

Working with medium-sized volumes and unsure which microtube to choose? With the ability to process samples from 0.5 mL - 5 mL, Eppendorf Tubes 5.0 mL fill the gap between alternative tube formats and conical tubes for larger volumes up to 5.0 mL.

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Product Information

Technical Data

Applications

Features

Forget impractical and inconvenient processing of sample volumes and find the right vessel for sample volumes up to 5 mL. Eppendorf microtubes are the benchmark for simple and safe sample preparation with options for an easy-access snap cap or a secure screw cap.

Superior microtubes with unrivalled quality

Ensure your DNA and protein data is as reliable as can be! Eppendorf microtubes are manufactured without the use of slip agents, plasticizers and biocides preventing contaminating leachables interfering with quantification assays.

Graph showing UV absorbance spectra of pure water incubated for 30 mins at 90 °C in tubes from different manufacturers. The data shown was generated using the 5.0 mL snap cap format. For additional information and protocol description please see Application Note 264.

The 5 mL microtube, a complete system

The 5 mL microtube follows 50 years of the Eppendorf microliter system that included the 1.5 mL microtubes, our renowned Thermomixer, Microcentrifuge and Eppendorf pipettes. Building on this established and ever-popular concept, Eppendorf also offers the 5.0 mL system, with componenets that go hand-in-hand with our 5 mL microtubes to help streamline your workflow.

• Eppendorf Tubes^® 5.0 mL
• Space-saving Eppendorf tube rack and storage box
• Thermomixer-compatible thermoblocks.
• 5 mL pipettes (manual and electronic) and epT.I.P.S. pipette tips
• Universal centrifuge adapters, including high-speed options, for use in existing rotors
• Adapters for use in epMotion automated workstation

The original 5 mL system from Eppendorf offers many benefits to help you work with medium-sized volumes up to 5 mL.

• Simplified sample processing
• Reduced risk of contamination - easy access to sample and a lower risk of spillage
• Convenient for the preparation of mastermixes and buffers – no more splitting large volumes between several tubes!
• Suitable for cell-based applications – sterile and manufactured from the highest quality materials
• Space-saving – forget processing 5 mL samples in 15 mL conical tubes! Eppendorf 5 mL microtubes fit the gap where others don’t
• Higher DNA yields, even from samples with a limited quantity of source material (see Application Notes 262 and 263)

Graph: Superior DNA yields – low-copy plasmid DNA recovery can be significantly improved by increasing the culture volume. The Eppendorf microtube 5.0 mL minimizes the risk of contamination by eliminating the need to divide samples across smaller tubes.

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Eppendorf Tubes® 5.0 mL - Microtube

Tubes 5.0 mL with screw cap and snap cap ACT Label

Eppendorf Tubes® 5.0 mL placed in centrifuge rotor buckets

Eppendorf microtube® 5 mL tube clip

Eppendorf microtubes® 5 mL placed in reservoir rack module

Eppendorf microtube® 5 mL next to 1.5 mL microtube and 15 mL conical tube

Eppendorf microtubes® 5 mL in high speed rotor

Eppendorf microtubes® 5 mL in amber with closed lid

Eppendorf microtube® rack with 5 mL and 1.5 mL tubes.

Eppendorf Microtube® 5 mL inside conical bottom centrifuge adaptor

5 mL microtubes in the Eppendorf ThermoMixer® C

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

5 mL Eppendorf microtube®, open

Eppendorf Tubes® 5 mL with screw cap and snap cap open

5 mL microtubes - screw cap, snap cap and amber

The ultimate screw cap microtube for sample security.

Screw cap microtube 5 mL with Eppendorf stamp

Eppendorf Storage Box: Space for up to 5 x 5 Eppendorf Tubes 5 mL

Tubes 5.0 mL screw cap, amber and transparent

Light Protection as well as Transparency

Selection of Eppendorf tubes® including microtubes in 0.5 mL, 1.5 mL, 5 mL format, and conical tubes in 15 mL and 50 mL format

Tradeshow Animation

Tubes 5.0 mL

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Product Information

Superior microtubes with unrivalled quality

The 5 mL microtube, a complete system

The original 5 mL system from Eppendorf offers many benefits to help you work with medium-sized volumes up to 5 mL.

Eppendorf Tubes^® 5.0 mL - Microtube