How can I decontaminate the Move It lower part?

With Xplorer plus, the lower parts are autoclavable. With Research plus the complete pipette is autoclavable.

The Move It lower parts show indications for plates. I have a plate which does not fit into these formats. Can I nevertheless use the Move It pipettes?

First, please check with the option table, if you have the suitable Move It pipette availabe. Maybe you simply need a different model for your applications. In case you have an atypical vessel format you can of course also use Move It if not exceeding the distance range. Use a ruler to check which Move It model is suitable for your atypic vessel type. Within this range all distances can be set. Therefore please proceed as follows: 1) Attach tips 2) Set the two format limiters to maximum width range 3) Use the rotating knob to adjust the space between the tips until fitting to the atypic vessel 4) Set one of the format limiter to this position 5) Set the rotating knob to the second vessel type 6) Set the format limiter to this tip distance You can now work between these set limits by simply using the rotating knob.

Which vessel formats are compatible with the Move It pipette?

Depending on Move It model, it can be used with labware with a vessel distance from 4.5 - 33 mm. Some examples: Plates (384- / 96-/ 48-/ 24-/ 12-well formats), 0.2 / 0.5 / 1.5 / 2.0 / 5 mL tubes, different vials, etc. Check the Selection Guide in our Move-It brochure to find the most suitable model for your specific workflow and vessel distance. The cone distance adjustment is stepless to ensure maximum flexibility also for non-standard vessel formats.

What is reverse pipetting using an air-cushion pipette? Is there literature available?

Unlike forward pipetting, reverse pipetting involves pressing the pipette button to the second pressure point, allowing a greater volume to be aspirated into the tip. When dispensing liquid, there’s no pipette blow-out; instead, the residual liquid remains in the tip. This technique offers enhanced accuracy and precision for pipetting liquids with high viscosity or those prone to foam or produce bubbles. Eppendorf offers a variety of literature explaining the technique and proving application examples, such as the Userguide 020, several webinars, and YouTube videos.

What is pre-wetting using an air-cushion pipette, and is it volume-independent?

Regardless of the volume, the air cushion should be wetted by multiple filling and emptying of the tip before dispensing.

What are effective techniques for pipetting viscous liquids?

When pipetting viscous liquids, we recommend positive displacement instruments such as the Multipette (M4, E3, E3x). Those instruments offer the best dosing accuracy for viscous liquids due to the piston integrated into the tip. When handling viscous liquids with an air-cushion pipette (Research plus, Reference 2), be sure to pipette slowly and use reverse pipetting (see Userguide 020 or our extensive eBook on www.eppendorf.com/Pipetting-eBook for guidance). Additionally, adjust your pipette settings to account for differences in density by using the temporary adjustment. However, results will not match those achieved with a positive displacement device.

Are your pipettes free of nucleic acids?

We cannot guarantee that our pipettes are completely free of nucleic acids. While we maintain high standards of cleanliness and care during production, we do not implement specific measures to prevent nucleic acid contamination. To protect your samples from potential contamination, we recommend using positive displacement devices or filter tips, such as ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax.

When should I grease my Eppendorf pipettes?

The piston or cylinder in the lower part of the pipette should be relubricated after cleaning, decontamination, or exposure to grease-dissolving solutions such as organic solvents. Re-greasing your pipette after autoclaving is not necessary.

How should warm or cold liquids be pipetted?

Best Practices for Pipetting Warm or Cold Liquids: Warm and cold liquids can behave differently than those at room temperature, requiring adjustments in pipetting techniques. Temperature differences between the liquid and the pipette can affect the air cushion, resulting in inaccuracies and imprecision that may exceed permissible error limits. To achieve optimal results, ensure that the pipette, tips, liquid, and surrounding air are all at the same temperature. For enhanced accuracy and precision, consider using a positive displacement device like the Multipette M4 or E3/E3x.

Can the heat of my hand affect my pipetting result?

Modern pipettes address this issue by ensuring sufficient spatial separation between the piston and the handle. Additionally, the materials used in our pipettes support thermal shielding/isolation to prevent heat transfer from your hand (see Userguide 021).

Do Eppendorf pipettes always have to be kept in a stand or can they also be put down on the laboratory bench?

The pipette can be placed on the laboratory bench, but never with liquid inside the tip. In general, it is best to keep pipettes on the pipette carousel. When storing pipettes in a drawer, ensure the pipette does not come into contact with the drawer walls or other equipment, as this may damage the pipette when the drawer is opened or closed.

How can I adjust my air-cushion pipette for challenging liquids, long tips, or higher/lower altitudes?

Upon manufacturing, pipettes are calibrated to distilled water. Eppendorf mechanical pipettes allow temporary user adjustments, beneficial for liquids with different physical properties than water (density, viscosity, surface tension, vapor pressure), or when capillary actions occur (e.g., with DMSO). Adjustments are also beneficial when atmospheric pressure changes due to altitude or when using non-standard pipette tips (e.g., epT.I.P.S. long). The adjusted pipette delivers the correct volume only for the liquid used during adjustment. Please be aware that variable mechanical pipettes turn into "fixed-volume pipettes" when being adjusted. That means they can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. Research plus and Reference 2 pipettes feature an adjustment window where the change can be monitored. It can be set back to factory settings "0". For detailed procedures and examples, refer to the operating instructions and Application Note No. 198. In contrast to mechanical pipettes, the electronic Xplorer/Xplorer plus pipettes can be adjusted for the entire range of volume. Additionally, the electronic pipettes offer several options for adjusting the pipette in the "Options" menu, e.g. to 75 % ethanol, altitude, elongated tips. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings. For more information see User Guide 036. Temporary user adjustments can be reset to manufacturer settings without recalibration.

How do I determine the volume values when calibrating gravimetrically?

The most common method involves measuring the mass of distilled water (whose physical properties are known) using a balance, and then converting the mass to a volumetric value. This means that the measurement values from the analytical balance are multiplied by the correction factor Z (see SOP pipettes). This adjustment accounts for the density of water at the specific temperature and the relevant air conditions.

What does EN ISO 8655 cover?

ISO 8655-2022 is the global standard for piston-operated volumetric apparatus (POVA), including pipettes, burettes, dilutors, dispensers, and manual precision syringes, as defined by the International Organization for Standardization (ISO). It ensures standardized performance for manufacturers, calibration providers, and users.It is structured in 10 chapters: ISO 8655-1: Terminology, general requirements and user recommendations ISO 8655-2: Pipettes ISO 8655-3: Burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric reference measurement procedure ISO 8655-7: Alternative measurement procedures ISO 8655-8: Photometric reference measurement procedure ISO 8655-9: Syringes ISO 8655-10: User guidance, user qualification and POVA qualification.

What do the terms random error and systematic error mean in a pipette calibration?

In pipette calibration, random error and systematic error are two key concepts that help ensure accuracy and precision in measurements. Random Error refers to the variability in measurements when the same volume is dispensed multiple times. It is also known as imprecision and is determined by calculating the repeat standard deviation of 10 measurements. These errors tend to scatter around the true value and can be minimized by consistent technique and proper training. Systematic Error is the consistent deviation of the measured volume from the selected/true volume, also known as inaccuracy and is determined by establishing the mean value of 10 measurements. Systematic errors can arise from defective or misadjusted devices and need to be corrected through proper calibration and maintenance.

At which volumes should a variable pipette be calibrated?

According to the EN ISO 8655-2022 standard, three series of 10 measurements are used to calibrate a variable-volume pipette. The three test volumes are: 1. Nominal volume (the largest volume adjustable by the user, as determined by the manufacturer) 2. 50% of the nominal volume 3. 10% of the nominal volume, or the lower limit if the minimum is over 10%

What is the nominal volume of a variable pipette?

The nominal volume of a variable pipette is always its maximum volume. For a 100 - 1,000 µL pipette, this means 1,000 µL.

I very frequently work with different chemicals. Can I still perform pipette calibration using water?

As per EN ISO 8655-2022, pipette calibration, which involves checking liquid dispensed by the pipette on a balance, should always be performed using distilled or deionized water. If you are working with chemicals that have different properties than water, consider using the temporary adjustment to minimize errors as outlined in Application Note 198. In general, we recommend using direct displacement devices such as the Multipette M4/E3(x) for challenging liquids.

How are multi-channel pipettes tested?

According to ISO 8655-2022, every channel of a multi-channel pipette must be tested. While all channels need to be filled, only the channel being tested is measured. Due to the high number of measurements required (e.g., 240 measurements for an 8-channel pipette and 360 measurements for a 12-channel pipette), it is advisable to send the multi-channel pipette to a professional calibration lab. These labs are equipped with specialized balance setups that ensure all channels are filled while only one channel is measured at a time.

Is the pipette grease free of resins?

Yes, the pipette grease supplied by Eppendorf is free of resins.

What material is my pipette made of and what chemicals is it resistant to?

Please refer to the Operating Manual on our homepage for information on material or chemical resistance before use. The Research plus and Reference 2 have separate chemical resistance sheets. Please note that aggressive chemicals used over a longer period of time may shorten the lifetime of the pipette.

What is the difference between factory setting and user adjustment?

Eppendorf pipettes offer factory adjustment and temporary adjustment. The temporary adjustment is an easy way of adjusting the pipette temporarily to different conditions (e.g. pipetting a liquid with high density). The factory adjustment should be changed if the pipette was found to be out of specifications during calibration. Before adjusting, exclude other causes such as pipette handling, environmental errors, or worn-out parts.

How often should pipettes be calibrated?

The user determines the frequency of calibration. However, according to EN ISO 8655-2022 Part 1, calibration must be performed regularly and at a minimum, once per year. Routine tests should be performed more frequently, ideally at intervals shorter than those for calibration. The frequency of both calibration and routine tests should be established through a risk assessment. This assessment needs to consider factors such as the frequency of pipette use, the number of users, the aggressiveness of the liquids being dispensed, and the acceptable error limits set by the user. Additionally, any industry- or organization-specific frequency requirements must be adhered to.

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Move It^® Adjustable Tip Spacing Pipettes - Adjustable Pipette

Move It® adjustable multi-channel pipettes from Eppendorf, with electronic and mechanical micropipettes shown

Move It® adjustable tip spacing multi-channel pipettes are the ideal dispensing tool when switching vessel formats

Illustration highlighting the transfer of samples from tubes to a 96-well plate or agarose gel

Tubing-free connections of cone and piston-cylinder system in adjustable tip spacing multichannel pipette

Mechanical Eppendorf Research® plus Move It® adjustable tip spacing pipette with 8 channels

Eppendorf Xplorer® plus Move It® electronic pipette with 6 adjustable channels

Move It® Adjustable Tip Spacing Pipettes: Transparent Lower Part in Action (GIF, Frontal View)

Move It® Adjustable Tip Spacing Pipettes: Transparent Lower Part in Action (GIF, Side View)

Female Scientist using Eppendorf Research® plus Move It® Pipette for Easy Liquid Transfer Between Vessel Formats

Scientist using a Move It® adjustable tip spacing pipette to transfer liquid from a series of tubes to a plate

Scientist using a Move It® adjustable tip spacing pipette to dispense liquid into a 384-well plate

The software of your Eppendorf Xplorer® plus pipette can easily be updated using the Pipette Software Update Tool

Product Information

Do you frequently transfer samples across different vessel formats, such as between tubes and plates, and strive for more efficient and safe solutions? The Eppendorf Move It is a multi-channel pipette with adjustable spacing between tip cones, accelerating and simplifying synchronous pipetting of a series of samples. A unique design without tubing connections results in increased precision and durability as well as user and sample safety.

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Easily update the firmware of your electronic pipette with our Pipette Software Update Tool

You will find additional download material at the bottom of this page

Product Information

Applications

Features

Double your pipetting performance with adjustable spacer pipettes

Eppendorf redefined adjustable multi-channel pipettes from scratch according to their premium standards. Move It is the result of customer requests for an efficient and safe solution for synchronous pipetting of a series of samples between different vessel formats, such as between tubes and plates. What is unique about the Move It? This adjustable multi-channel pipette can switch its fit for different vessel formats without any tubing connections between cone and piston-cylinder system. This significantly enhances pipetting performance: Fewer movable, often fragile parts, means more precision and durability. Move It is autoclavable which increases user and sample safety.

Watch our Move It adjustable tip-spacing pipette in action in our product video.

One pipette – Many vessel formats

Move It adjustable spacing multi-channel pipettes significantly accelerate and simplify the workflow when frequent format changes are required. Let’s get ready to move a series of samples synchronously between different vessel formats, such as between tubes and plates. The operating time is significantly less than when using single-channel pipettes. For microplates, tubes, and agarose gels, the tip spacing can be freely selected between 4.5 and 33 mm depending on the model.

Save up to 70 % of your time when filling 384-well plates

One of the most efficient ways to manually fill a 384-well plate* is a combination of liquid medium distribution with an Eppendorf Xplorer plus 24-channel pipette and synchronous sample transfer with the Xplorer plus Move It pipette. Ask your sales representative for demo devices and see for yourself!

*results were measured by using 12-channel mechanical pipettes for liquid medium distribution and single-channel pipettes for individual sample transfers.

Easy and fast tip spacing adjustment

With an Eppendorf Move It adjustable pipette, source and destination formats can be adjusted and locked with the format limiter which quickly switches forward and backward within the set formats. This means less concentration is required when setting the next pipetting step, saving valuable time. With these Eppendorf adjustable spacer multi-channel pipettes, the format adjustment itself is carried out manually with an adjustment knob which allows for a quicker adjustment than via a motor. The format adjustment process is designed to allow the user to always maintain a relaxed body and hand position.

Tubeless adjustable cone system for reliability and safety

The Eppendorf way: Move It adjustable spacing multi-channel pipettes work without any tubing connections between cone and piston-cylinder system. This design offers special advantages compared with conventional instruments:

• Potential problems caused by the presence of tubing, such as porosity and leaks, are not an issue here. Instead, fewer moving parts in Eppendorf adjustable multi-channel pipettes increase precision and durability
• By eliminating tubing, the size of the air cushions is reduced to a minimum which has an additional positive effect on precision and accuracy
• Move It can be autoclaved (Research plus fully, Xplorer plus lower part only) which additionally increases user and sample-safety

Rotatable pipette head – 360°

An ergonomic optimization of your workplace can help to make workflows more streamlined. As Move It adjustable cone spacing multi-channel pipettes are designed without internal tubes, the pipette head is 360° rotatable. The user benefits from comfortable readability of the display. This not only supports an ergonomic and relaxed body posture. At the same time, it ensures fast identification of all parameters saving valuable time at the bench.

Optimal balance for a relaxed hand

Need a break? With a natural and relaxed hand position, the demand for breaks decreases. Move It pipettes reduce hand and body fatigue by perfectly balancing in your hand. This is achieved through an optimized design. Users also benefit from convenient visibility of tips and samples, allowing for stress-free operation all day long.

epT.I.P.S.^® 384 with SoftAttach^® technology for Move It 20 µL and 100 µL variants

The innovative epT.I.P.S. 384 tips for Eppendorf Move It pipette models with nominal volumes of 20 or 100 µL are equipped with elastic forming groves. These allow the tips to stretch as much as the shape of the tip cone predefines. Meanwhile, the tip attachment and ejection forces are greatly reduced. With this latest expression of the Eppendorf PhysioCare Concept, this system enables a perfect tip seal while operating forces are reduced to a minimum.

Your benefits:
• Reliable tip fit and tightness
• Smooth and even tip attachment
• Tip attachment forces reduced by up to 40 % per cone compared to standard tips
• Reduced ejection forces
• Perfect tip alignment due to extraordinary coaxiality for safe plate maneuvering and liquid transfer
• Extremely fine tip shape

Move It adjustable tip spacing pipettes selection guide

With the selection guide, it is possible to choose the right Eppendorf adjustable multi-channel pipette for every application at a glance. Select source and destination format according to your application and then select the channel and volume-variant best suited to your application. Each adjustable pipette variant is available as mechanical Eppendorf Research^® plus Move It or electronic Eppendorf Xplorer^® plus Move It pipette.

Additional benefits with the Xplorer plus Move It variants
Compared to mechanical pipettes, electronic pipettes have a number of advantages in many applications. The most important benefits of using an electronic pipette are: better ergonomics by requiring almost no operating forces, a higher precision and reproducibility and an additional efficiency gain due to various operating modes (such as e.g. pipetting and dispensing with only one tool). With the Eppendorf Pipette Software Update Tool you can easily update your electronic pipette and stay up to date with the latest software improvements and new features! Learn more at Eppendorf's dedicated microsite about electronic pipetting.

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Downloads: Move It^® Adjustable Tip Spacing Pipettes

Selection Guide

Get It Right! Selection Chart for Liquid Handling Instruments & Tips

Find the most suitable pipetting system for your lab applications

Unterschiedliche Gefäßformate? Schnell und sicher mehrere Proben gleichzeitig transferieren: Eppendorf Research^® plus und Eppendorf Xplorer^® plus Move It^®

Eppendorf Research^® plus and Eppendorf Xplorer^® plus Move It^® adjustable tip spacing pipettes
Fast and secure transfer of multiple samples between different formats

PDF 2.6MB

English

Flyer

Increase Your Pipetting Productivity Today

Higher throughput and increased reproducibility with Eppendorf multi-channel pipettes

You give your all to scientific research every day. Eppendorf liquid handling instruments help you grow beyond your limits

Eppendorf Instrumente für das Liquid Handling:
Konkurrenzlos in Technologie, Effizienz und Handling – seit über 50 Jahren.

PDF 8.3MB

German

Brochure

Pipette Service Done Right

Eppendorf Pipette Calibration

PDF 3.0MB

English

Brochure

Pipettenservice - Richtig & Wichtig!

Eppendorf Pipettenkalibrierung

PDF 2.8MB

German

Selection Guide

Suitable Move It^® Adjustable Tip Spacing Pipette Model by Vessel Format

Measure tube distances to determine the suitable Move It variant for your application

PDF .4MB

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Flyer

Simply Better Pipetting

With the Eppendorf Xplorer^® electronic pipette family

PDF .2MB

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Elevate Your Laboratory Standards

ISO standards for calibration of liquid handling devices

PDF 1.2MB

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Move It® Adjustable Tip Spacing Pipettes - Adjustable Pipette

Move It® adjustable tip spacing multi-channel pipettes from Eppendorf

Move It® adjustable tip spacing multi-channel pipettes are the ideal dispensing tool when switching vessel formats

Quickly move samples across different vessel formats – with ease!

Increase precision, accuracy, and durability with our adjustable tip spacing multichannel pipette

Eppendorf Research® plus Move It® 8-channel pipette, 5 – 100 µL, light yellow

Eppendorf Xplorer® plus Move It® 6-channel pipette, 15 – 300 µL, orange

Move It® adjustable tip spacing multi-channel pipettes work without any tubing connections between cone and piston-cylinder system

Easily and quickly adjust tip spacing with a manual adjustment knob

Female Scientist using Eppendorf Research® plus Move It® Pipette for Easy Liquid Transfer Between Vessel Formats

Accelerate and simplify synchronous pipetting of a series of samples and experience a new joy in the lab!

Choose Move It® pipettes with a minimum cone spacing of 4.5 mm when working with 384-well plates

The software of your Eppendorf Xplorer® plus pipette can easily be updated using the Pipette Software Update Tool

Move It® Adjustable Tip Spacing Pipettes: Product Video

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Pipetting and Mixing with Eppendorf Xplorer® (plus) Pipettes

Reverse Pipetting with Eppendorf Xplorer® plus Pipettes

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Temporary Adjustment of Eppendorf Xplorer® (plus) Pipettes