Cell Culture FAQ: How to handle various cell lines?

Lab Academy 20. August 2019

Cell culture work often implies the cultivation of various cell lines for different ongoing experiments. The simultaneous nourishment of different cell lines in a biosafety cabinet might save some time during a busy day in the laboratory but dramatically increases the risk of cross-contamination and can thus seriously impair the entire experiment. Therefore, some aspects should be considered when working with different cell lines:

Work with only one cell line at a time in the biosafety cabinet.
Use labeled aliquots of media and reagents for each individual cell line.
Disinfect the interior of the biosafety cabinet after a work session with one cell line.
Run the laminar flow for a few minutes between work sessions with different cell lines.

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CO2 Incubators – Best Practices for Selection, Set-up and Care

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Cell Culture FAQ: How to handle various cell lines?

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