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Discover the potential of the Mastercycler® X40 in your own lab.

Flexible, Productive and Easy-To-Use

The Eppendorf Mastercycler® X40 leads your standard PCR into a new era. The performance of the reliable aluminum block is developed for excellent temperature homogeneity. A 12-column gradient offers fast PCR optimization. You can use the gradient in whatever temperature step you wish, e.g. denaturation or annealing.

The lid handle of Mastercycler® X40 can be operated ergonomically with one hand and the SafeLid adapts to apply the perfect pressure on your samples for evaporation protection.

0.1 mL, 0.2 mL tubes and tubestrips, or any type of PCR plate – skirted, semi-skirted and unskirted plates – can be used. All in a thermal cycler that is only slightly larger than a piece of paper and weighs just 7.25 kg.
All Mastercycler® X40 programming is done fast, intuitive, and comfortably on the chemical resistant glass surface touchscreen.

Software benefits:
  • Program migration feature: transfer protocols from other slower cycler models. The ramp rates automatically adjust to the total runtime your other cycler needed for the PCR run, so that you can start right away.
  • Well-established user interface: multiple programming options for ideal conditions of different types of PCR protocols, e.g. 3-step, nested, or gradient PCR.
  • 4 temperature modes enable you to always have the ideal temperature conditions for your sample volume.
Left profile, front view and right profile of a Mastercycler X40 with labeling of components.

Get flexible, productive and optimized

Flexibly optimize your PCR with a 12-column temperature gradient and raise productivity by switching from PCR tubes to plates. The SafeLid automatically applies perfect pressure on the consumables to prevent evaporation. Each plate type fits the block ideally, so that you can also choose automated systems for up-, or downstream processing.

Become digital


The intuitive software and touchscreen offer easy programming and tracking runs. Clear user management guarantees simple handling of protocols. The integrated VisioNize® connectivity enables you to use VisioNize® Lab Suite and monitor, book or audit multiple devices in your digital lab.

Topview on PCR program of Mastercycler X40