No results found
Search Suggestions
With more and more developments in the cell and gene therapy, there is an increasing need for stem cells or stem cell derived products such as exosomes. In the workshop, we will share insights into strategies on how to cultivate stem cells in stirred rigid-wall bioreactors, discuss the importance of monitoring, control, and process automation and share hands-on advices for exosome isolation. Most eukaryotic cells release membrane-derived vesicles, also called extracellular vesicles (EVs) that can have an impact on both neighboring and distant cells. EVs are spherical cytosol fragments surrounded by lipid-bilayer membranes and hydrophilic proteins, similar to cell plasma membranes, and composed of various bioactive molecules, including RNAs, DNAs, proteins, mRNA, microRNA, and lipids. With the increasing interest in the research applications of exosomes, it becomes more and more important to require reproducible methods of cultivation and purification of EVs.
Important information
The workshop will be held live (no virtual participation possible)
Target group: Post doctoral researcher, PhD students, lab technicians and everyone who is afraid of growing stem cells in a stirred system
This workshop is limited to 30 participants. A waiting list will be offered in case the workshop is already fully booked. Please send an email to the Conference Office if you would like to be included on the waiting list.
Date & Time: Monday, November 20, 2023 | 03:00 pm - 05:30 pm
(followed by an informal Get Together with drinks and flying buffet)
Venue: Van der Valk Hotel Sassenheim - Leiden
Target group: Post doctoral researcher, PhD students, lab technicians and everyone who is afraid of growing stem cells in a stirred system
This workshop is limited to 30 participants. A waiting list will be offered in case the workshop is already fully booked. Please send an email to the Conference Office if you would like to be included on the waiting list.
Date & Time: Monday, November 20, 2023 | 03:00 pm - 05:30 pm
(followed by an informal Get Together with drinks and flying buffet)
Venue: Van der Valk Hotel Sassenheim - Leiden
Trainer
Recommended reading
F. Manstein et al., “High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling,” Stem Cells Transl. Med., 2023.
F. Manstein, K. Ullmann, W. Triebert, R. Zweigerdt, “Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors” STAR Protocol 2021 Dec.
H. Kempf, C. Kropp, R. Olmer, U. Martin, and R. Zweigerdt, “Cardiac differentiation of human pluripotent stem cells in scalable suspension culture,” Nat. Protoc., vol. 10, no. 9, pp. 1345–1361, 2015.
F. Manstein, K. Ullmann, W. Triebert, R. Zweigerdt, “Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors” STAR Protocol 2021 Dec.
H. Kempf, C. Kropp, R. Olmer, U. Martin, and R. Zweigerdt, “Cardiac differentiation of human pluripotent stem cells in scalable suspension culture,” Nat. Protoc., vol. 10, no. 9, pp. 1345–1361, 2015.
