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DNA LoBind® Tubes
Eppendorf LoBind® - How it works
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Product Information
Maximize nucleic acid recovery! Eppendorf LoBind® Tubes signifcantly reduce sample-to-surface binding to ensure maximal recovery of DNA and RNA molecules. The ideal solution for sample preparation and long-term storage of your precious nucleic acids!You will find additional download material at the bottom of this page
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Product Information
Applications
Features
Product Information
Minimize sample loss and maximize the recovery of precious nucleic acids with Eppendorf LoBind® Tubes. Combining superior manufacturing technologies and high-quality polypropylene, the LoBind® tubes offer almost 100 % recovery of DNA and RNA molecules without the need for surface coating.
Rest assured that Eppendorf LoBind® Tubes are of the highest quality and suitable for all applications including forensic analysis, microarray and next generating sequencing (NGS). LoBind® tubes undergo stringent batch-testing and certification by an independent laboratory to ensure they are free from DNA, DNase, RNase, and PCR inhibitors
Rest assured that Eppendorf LoBind® Tubes are of the highest quality and suitable for all applications including forensic analysis, microarray and next generating sequencing (NGS). LoBind® tubes undergo stringent batch-testing and certification by an independent laboratory to ensure they are free from DNA, DNase, RNase, and PCR inhibitors
An Eppendorf LoBind® solution for every problem!
Eppendorf DNA LoBind® is available in a variety of formats - from standard tubes to microplates and deep well plates - to help meet your specific needs, whether that’s low sample volumes or increased throughput.
Unparalleled DNA recovery rate
Eppendorf LoBind® Tubes recover up to 99 % of DNA, independent of incubation temperature and time, when compared to competitor tubes. Graph shows DNA recovery rate (0.2 ng/µL DNA fragment (130 bp, 32P labeled) in 2.5 M NaCl/TE buffer) in Eppendorf LoBind® Tubes and competitor tubes after various incubation conditions.
Eppendorf LoBind® Tubes offer greater recovery potential when compared to a low bind competitor tube. An assessment of Eppendorf LoBind® Tube consistency in the preparation and storage of standards for real-time PCR absolute quantifications showed that the low adsorption of DNA to LoBind® Tubes improved the quality of standard curves and DNA quantification in assayed samples.
Image: Preparation of DNA standards: Genomic DNA stock solution was serially diluted 10–fold in competitor low bind tubes or Eppendorf DNA LoBind® Tubes. 22 µL aliquots of each dilution were stored for 24 hours at –80 °C before analysis by real-time PCR. Samples stored in Eppendorf LoBind® Tubes had a greater PCR efficiency when compared to the competitor. See Application Note 226 for more details.
Image: Preparation of DNA standards: Genomic DNA stock solution was serially diluted 10–fold in competitor low bind tubes or Eppendorf DNA LoBind® Tubes. 22 µL aliquots of each dilution were stored for 24 hours at –80 °C before analysis by real-time PCR. Samples stored in Eppendorf LoBind® Tubes had a greater PCR efficiency when compared to the competitor. See Application Note 226 for more details.
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Applications
- Preparation or storage of DNA and RNA samples
- Forensic trace analysis
- Preparing dilution series in quantitative qPCR
- Sample preparation in next-generation sequencing
- Creation of genomic or oligonucleotide libraries
Features
- Eppendorf LoBind material ensures optimized sample recovery for improved assay results
- Free of surface coating (e.g., silicone) to minimize the risk of sample interference
- Lot-certified PCR clean purity grade: free of human DNA, DNase, RNase and PCR inhibitors
- Available in tube, microplate, and deepwell plate formats for easy-up scaling
- Precise lid sealing for minimal evaporation rates in tube format
- Rated up to 30,000 × g (25,000 × g for 2.0 mL tube) centrifugation speed for molecular biology applications