Why does the control-LED go out when I change the parameters of a soft key?
Because the altered values no longer match the parameters assigned to that soft key the control-LED light goes out. This prevents any confusion bewteen the mixing parameters.
Why do you recommend that I mix deepwell plates at max. 2,000 rpm?
Depending on geometry and filling volume, DWPs might have a high balance point and heavy weight, respectively. Due to safety reasons, the imbalance detector may be activated at mixing frequencies above 2,000 rpm and automatically reduce the mixing frequency (to avoid plates jumping out of the Universal Holder).
Why do you recommend mixing at max. 2,000 rpm when using the tube holders?
Because of their high balance point and their maximum capacity (e.g., Tube Holder 1.5/2.0 ml), the imbalance detector might be activated at mixing frequencies above 2,000 rpm. This automatically reduces the mixing frequency to prevent the tubes and the tube holder from jumping out of the Universal Holder.
How can MixMate mix samples in a controlled manner, without any lid wetting?
The interplay of several factors that influence on the mixing motion has been optimized, in particular: The minimization of vertical motion, the operating smoothness and as well as the sturdy plate/tube holders. All serve to prevent any uncontrolled, chaotic movement and thus ensure an optimum transfer of mixing energy from the device to the sample. Still, the risk of any lid wetting is dependent on outside factors such as tube size, chosen mixing speed, filling volume and the properties of the sample material (e.g., buffer composition, viscosity, density), etc. To easily configure your optimum mixing parameters despite such variations, MixMate is equipped with five preprogrammed soft keys to enable fast and controlled mixing of various plate and tube formats in standard applications.
Why does the vortexer continue to run for approx. 2 seconds after relieving pressure on the vortex mat?
This 2-second overrun makes the vortexing process more ergonomic, because the vortex mat need not be pressed continuously to keep the vortexer running. It also eases the process of performing vortexing in several tubes successively.
My plate does not fit into the Universal Holder, why?
The Universal Holder can only accommodate plate formats that comply with the SBS Standard for Microplates
Which cleaning and disinfection agents can be recommended for cleaning and disinfection of ThermoMixer C, ThermoMixer F and ThermoStat C?
Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes. Do not use any aggressive chemicals on the device or its accessories, such as strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol. If the device becomes contaminated with aggressive chemicals, clean it immediately with a mild cleaning agent. Use Lint-free cloth, mild, soap-based lab cleaner and dist. Water. Please refer to your operating manual chapter Maintenance.
Is the housing of ThermoMixer C, ThermoMixer F and ThermoStat C stable against UV irradiation?
A disinfection by UV irradiation cannot be recommended, because discoloration of the housing materials can occur.
Which DNA and RNAse deconatmination agents can be recommended for decontamination of the devices and the SmartBlocks?
We tested DNA away and RNase away and we can recommend to use both agents.
Which decontamination agent do you recommend to remove radioactivity from the devices and SmartBlocks?
We recommend to use Count off.
Are start and end position of the SmartBlocks the same?
No. Start and end position are not identical.
How can the temperature accuracy be verified?
The best possible temperature accuracy can be guaranteed by individually adjusting a SmartBlock. Optimized temperature control for each block type keeps temperature overshoots and deviations caused by external interferences to a minimum. To guarantee optimal performance over the entire period of use, Eppendorf offers a preventive maintenance program and certification services: your Eppendorf ThermoMixers, ThermoStat C as well as the SmartBlocks are regularly cleaned, checked and adjusted. Additional information is available at: https://www.eppendorf.com/epservices
. Customers can also verify the temperature accuracy by themself. Eppendorf provides for that purpose the Temperature Verification System – Single Well for Eppendorf Mastercyclers (0056 000.003) in combination with the 384 well Sensor (0056 002.006).
Can cell culture plates be used in the SmartBlock plates?
Also cell cultuture plates can be used in the SmartBlock plates in so far the dimensions of the plate comply to the SBS standard.
Can ThermoMixer C, ThermoMixers F & ThermoStat C be operated with remote controle?
No. The devices can not be contolled remote via a PC.
Can ThermoMixer C, ThermoMixers F & ThermoStat C be used in a cold room?
Yes, one can use the devices in a cold room but some safety hints should be attended: Condensate can form in the device after it has been moved from a cool environment to a warmer environment. Wait at least 3 hours after positioning the device. Plug the device only after this time has elapsed. In case the device is positioned continually in a cold room and was switched off for a longer time – over night for instance - switch it on for at least 1 h before starting a mixing process.
Is it possible to put the SmartBlocks for a rapid cool down into a -20°C freezer?
The SmartBlocks can be used at ambient temperatures of 5°C – 40°C and can be cooled appropriate. Cooling down of SmartBlocks in a freezer should not be done.
ThermoMixer C; ThermoStat C: Is it possible to run more than one program in automatic succession?
No. Each program has to be chosen and run separately. But the 4 possible steps within a program run in automatic succession.
Which SmartBlocks and vessels can be used with ThermoMixers F?
The ThermoMixers F possess a fix installed SmartBlock and can only be used with the appropriate microtest tubes or plates, such as MTP’s, DWP’s or skirted plates complying the SBS standard. For using PCR plates our SmartBlocks PCR 96 and PCR 384 in combination with ThermoMixer C is the proper choice.
The ThermoMixer / ThermoStat shows the error message “ThermoTop not recognized”.
Please check if a compatible SmartBlock has been attached and the ThermoTop has been vertically placed from above. The centering pins behind the heating/cooling plate have to fit perfectly into the recesses of the ThermoTop. Place the ThermoTop once again upon the device. In case the device shows still the error message, please contact the technical service.
Do the IsoRacks fit upon the SmartBlocks for microreaction tubes (0.5 mL; 1.5 mL; 2.0 mL)?
No. The IsoRacks fit only upon the Thermoblocks for the older devices Thermomixer comfort, Thermomixer compact & ThermoStat plus.
Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?
SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.
Can the ThermoStat C be used in a cold room?
Yes, one can use the devices in a cold room but some safety hints should be attended: Condensate can form in the device after it has been moved from a cool environment to a warmer environment. Wait at least 3 hours after positioning the device. Plug the device only after this time has elapsed.
What does the term 2DMix-Control technology describe/stand for?
2DMix-Control technology describes the ability of the MixMate, ThermoMixer C and ThermoMixers F to mix small sample volumes in various plate and tube formats quickly and in a controlled manner. To achieve this, the interplay of all crucial factors for efficient and controlled mixing, have been optimized. And these are:
- Generation of sufficient mixing energy: due to an orbital mixing movement at a high mixing speed and a relatively large mixing radius (1.5 mm) the devices generate enough mixing energy (analogous to g-force in centrifuges) to mix even the smallest volumes efficiently.
- Minimized loss of mixing energy: special attention was paid to reducing inherent vibration. Smooth operation combined with reliable stability ensures that all the mixing energy is available for mixing and not wasted through transmission of vibrations to the device or its surroundings.
- Controlled transmission of energy from the device to the sample: Minimizing vertical movements enable a plane, 2-dimensional (2D) mixing stroke. This, in conjunction with the reliable plate and tube holders and SmartBlocks, prevents uncontrolled chaotic movements and allows all formats to be held securely, even at high mixing frequencies.
What types of applications can be carried out with Eppendorf Tubes® 5.0 mL?
Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur®. They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.
Is it necessary to buy new equipment to be able to use the new tube?
The bottom shape of the Eppendorf Tubes® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265
Is it possible to store samples in 5.0 mL Eppendorf Tubes® in the freezer?
Yes, the tubes can be stored down to -86 °C.
How resistant are the Eppendorf Tubes® 5.0 mL to chemicals?
The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.
Are the Eppendorf Tubes® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?
The Eppendorf Tubes® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.
Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?
It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.
What is the Tube Clip used for?
The Eppendorf Tubes® 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.