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1250 Dokumenty
How do I choose the correct glass bottom thickness?
How do I choose the correct glass bottom thickness?
Eppendorf Cell Imaging Dishes are available with two different cover glass bottom thicknesses: 145 µm (No. 1.0) and 170 µm (No. 1.5). However, for most applications 170 µm is the preferred thickness. Most microscope objectives are designed to be used with a cover glass that has a thickness of 170 µm. The cover glass thickness should correspond to the optimum thickness indicated on the microscope objective.
Do I have to fill the whole Cell Imaging Dish with culture media or is it enough when only…
Do I have to fill the whole Cell Imaging Dish with culture media or is it enough when only the glass bottom is covered with liquid? How are Eppendorf Cell Imaging Dishes typically used?
In general we recommend filling the whole dish with culture media to minimize evaporation and ensure optimal cell growth especially during long-term experiments. The special design of the imaging area as defined central cavity with a depth of 2 mm enables to concentrate cells conveniently in the imaging area and to use very low volumes of e.g. staining substances or antibodies. This helps to reduce the costs of your experiment. The following procedure ensures that the cells grow only within the imaging area: first seed the cells into the central cavity of the dish to restrict cell attachment to the imaging area (cell seeding volume 0.75 mL). After cells have been attached to the glass bottom, the whole dish can be filled with additional media (working volume 2.0 mL).
Can the cover glass bottom be removed from the dish?
Can the cover glass bottom be removed from the dish?
No, the cover glass bottom cannot be removed from the dish. The glass is fixed tightly to the dish and there is a risk of injury by breaking the glass when trying to remove it.
Can the chamber of the Eppendorf Cell Imaging Slide and Coverglass be removed without using…
Can the chamber of the Eppendorf Cell Imaging Slide and Coverglass be removed without using an additional tool?
Yes, the chambers of both – Slides and Coverglasses - can be removed without an additional tool. However, when working with the Imaging Coverglasses some users might find it more convenient to use e.g. tweezers or a pipette tip to fix the glass bottom when removing the chamber.
Are the chambers / lids made of polystyrene?
Are the chambers / lids made of polystyrene?
No, the chambers are made of COC (cyclic olefin copolymer), which is in contrast to polystyrene resistant to substances like acetone and is more stable to different temperatures.
Is the attachment of the chamber to the Slide / Coverglass leak-tight?
Is the attachment of the chamber to the Slide / Coverglass leak-tight?
Yes, Eppendorf guarantees the liquid-tightness of all Cell Imaging Consumables. As part of our Quality Control procedure each LOT of products is tested and certified as being leak-tight.
Can the chamber be reused once it is detached from the Slide / Coverglass?
Can the chamber be reused once it is detached from the Slide / Coverglass?
When the chamber is detached it cannot be fixed tightly to a microscope slide or a cover glass again. Apart from this we do not recommend re-using the chamber for any cell culture applications to avoid any risk of contamination because of non-sterile conditions.
What material is used for the Eppendorf Cell Imaging Plates with film bottom plates? …
What material is used for the Eppendorf Cell Imaging Plates with film bottom plates?
The film bottom of the Eppendorf Cell Imaging Plates is made of fluorocarbon, a material that provides very low autofluorescence and high gas transfer rates.
Can I use the Eppendorf Cell Imaging Plates for automated systems?
Can I use the Eppendorf Cell Imaging Plates for automated systems?
The Eppendorf 96-Well Cell Imaging Plates conform to the SBS format. Therefore they fit to most automated systems.
Can the Eppendorf Cell Imaging Plates be used in a centrifuge?
Can the Eppendorf Cell Imaging Plates be used in a centrifuge?
Yes, the Cell Imaging Plates can be centrifuged at a speed up to 300 x g. However, the centrifugation stability of each plate type generally depends on the centrifuge and its accessories, the ambient conditions and the liquid used.
What is the chemical resistance of Eppendorf Cell Imaging Products?
What is the chemical resistance of Eppendorf Cell Imaging Products?
Please find an overview about the chemical resistance of our Cell Imaging Consumables against the most important chemicals here.
What certificates are available for Eppendorf Cell Imaging Consumables?
What certificates are available for Eppendorf Cell Imaging Consumables?
In the attached table you can see the available certificates for our Cell Imaging Consumables.
What are the specifications of the Eppendorf Cell Imaging Dishes?
What are the specifications of the Eppendorf Cell Imaging Dishes?
Please find an overview on technical data, volumens and optical properties of the Eppendorf Cell Imaging Dishes in this table.
What are the specifications of the Eppendorf Cell Imaging Slides & Eppendorf Cell Imaging…
What are the specifications of the Eppendorf Cell Imaging Slides & Eppendorf Cell Imaging Coverglasses?
Please find an overview on technical data, volumes and optical properties of the Eppendorf Cell Imaging Slides & Eppendorf Cell Imaging Coverglasses in the table here.
What are the specifications of the Eppendorf Cell Imaging Plates?
What are the specifications of the Eppendorf Cell Imaging Plates?
Please find an overview on technical data, volumes and optical properties of the Eppendorf Cell Imaging Plates in the table here.
How does the shaker agitation rate and orbit affect culture growth?
How does the shaker agitation rate and orbit affect culture growth?
Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double〖RPM〗_1/〖RPM〗_2 = (r_2^2)/(r_1^2 ), then 〖RPM〗_2 = √(〖RPM〗_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm.
Do Eppendorf shakers come with platforms?
Do Eppendorf shakers come with platforms?
Each Eppendorf shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. only 250 mL clamps) and provide in most cases the best capacity but does not allow for the use of different size clamps or holders. Universal Platforms are designed for use with a combination of various-sized clamps and test tube racks providing maximum flexibility. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately.
What is the temperature offset function?
What is the temperature offset function?
The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.
I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration? …
I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?
The Eppendorf Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The Eppendorf Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine.
Why are some flask clamps supplied with an extra spring set?
Why are some flask clamps supplied with an extra spring set?
This "extra spring set" (we call it a girdle) is included with clamps for 2 L, 2.8 L, 4 L and 6 L flasks. It is intended to be used with the already installed spring set. This girdle helps prevent large flasks from spinning inside the clamp and reduces the risk of flasks becoming dislodged or scored by the steel clamp. One girdle is already in place on the clamp, the other is packed separately. To install this extra “girdle”, follow the instructions in the manual supplied with the shaker.
How is temperature accuracy defined by Eppendorf?
How is temperature accuracy defined by Eppendorf?
Accuracy is defined as the degree of closeness between the displayed value and the actual value as determined by a traceable device. All Eppendorf Incubated Innova and I-Series shakers have a temperature Accuracy of  ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range. New Brunswick Incubated Excella Shakers are accurate within 0.1°C at 37°C.
How is Uniformity defined by Eppendorf?
How is Uniformity defined by Eppendorf?
Uniformity is the ability to maintain uniform temepratures throughout the platform. New Brunswick Incubated Innova Shaker temperatures will not deviate by more than +/- 0.25 °C at 37 °C.
What is an orbit ?
What is an orbit ?
The orbit of a shaker is the distance traveled around the central drive. This can be descibed in inches, centimeters, or milimeters. If our shakers had a one inch (2.54 cm) orbit. That means that that the lateral movement of the platform would equal one inch from one point to another that is 180° opposite that position. All of the New Brunswick. shakers have a circular orbit
What wavelengths are used in your growth lamps in a photosynthetic light bank?
What wavelengths are used in your growth lamps in a photosynthetic light bank?
Each of the bulbs that is fit into our photobanks is a wide spectrum bulb that provides the red and blue wavelength of energy needed for photosynthesis. Innova 42R has six bulbs as the Innova 43R and 44R have nine bulbs. To have this option installed, the shaker needs to have the refrigeration option installed as well.
Which screws will fit to which platform ?
Which screws will fit to which platform ?
All of Eppendorf Inc. clamps and accessories are packaged with multiple screws to fix them to our universal platforms, included in M1013-0261 clamp screw kit (Stainless steel platform screw #S2117-3050 (10-32 x 5/16 in (7.9mm)), Stainless Steel, Aluminum, and Aluminum and Phenolic (Plastic) #S2116-3051 (10-24 x 5/16 in (7.9 mm)), and Wood #S2116-3101 (10-24 x 5/8 in (15.87mm)).
How can I disinfect, clean, maintain my shaker?
How can I disinfect, clean, maintain my shaker?
We recommend cleaning the exterior of our units with a damp cloth or any standard household or laboratory cleaner to wipe the surfaces clean. Do not use abrasive or corrosive compounds to clean the instruments as they may damage the unit and void the warranty. It is the responsibility of theuser to carry out the proper decontamination procedures if hazardous materials are spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should contact Eppendorf Inc. to ensure that the proposed method will not damage the equipment. Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends on the nature of the spill. Spills involving fresh cultures or samples of known concentrations of biomass should be flooded with decontamination solution and soaked for at least 5 minutes before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occuring in areeas warmer than ambient/room temperature, should be exposed to decontamination solution for at least one hour before being cleaned up. Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process.
Respiratory protection should be considered for spills where aerosolization is suspected.
Which flask type fits in which clamp ?
Which flask type fits in which clamp ?
Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.
Which vessel fits in Eppendorf Test tube racks ?
Which vessel fits in Eppendorf Test tube racks ?
Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the Userguides section of the shaker product page.
What is the humidity option ? Is the value of humidity shown in the display ? How can I…
What is the humidity option ? Is the value of humidity shown in the display ? How can I avoid evaporation?
The humidity monitoring option that is offered with our Innova line displays the Relative Humidity (RH) of the chamber. This value will be reported if the shaker is equiped with the humidity sensor. The value can be shown in the main display screen if programmed to do so or the value can be viewed with all other parameters under the SUMM (summary) screen.The calibrated sensor is accurate within 3 % up to 95 % RH and 5 % up to 99 % RH. To avaoid evaporation in the shakers, it is recommended to fill the humidification resevoir beneath the platform, this will mitigate loss of culture volume.
Why is the triple eccentric drive special ? What is the difference between Innova and…
Why is the triple eccentric drive special ? What is the difference between Innova and Excella Shakers drive ?
The Innova and I-series Triple eccentric drive consists of three individual bearings and eccentric shafts mounted in a triangular fashion for precise uniform circular motion at every point on the platform. The heavy duty drive has a single shaft that utilizes springs to aid in balancing the load. This drive works great with small to medium loads at lower speeds.