Do the Eppendorf Cell Imaging Consumables promote attachment and proliferation of adherent cells?
All Eppendorf Cell Imaging Consumables are tissue-culture (TC) treated for improved adhesion of cells on the glass (Eppendorf Cell Imaging Dish, - Slide, - Coverglass, - Plate with glass bottom) or film bottom surface (Eppendorf Cell Imaging Plate with clear film bottom).
Is there a difference regarding surface treatment between Eppendorf Imaging Consumables (cover glass, glass, film bottom) and Eppendorf Cell Culture Consumables (polystyrene)?
Both - Eppendorf Cell Culture Consumables and Eppendorf Cell Imaging Consumables are tissue-culture (TC) treated. Although the methods applied are slightly different due to different material properties of polystyrene, film and glass all TC-treatments are chemical modifications (no coatings) generating functional groups covalently bound to the base material. This surface modification results in an improved adhesion of anchorage dependent cells.
Do I have to use biological coatings like Fibronectin, Poly-D/L-Lysine or Collagen to make the cells adhere to the surface?
The surface of all Eppendorf Cell Imaging Consumables is tissue-culture (TC) treated meaning that it promotes the attachment of most adherent cell types. However, some sensitive primary cells or cell lines might make it necessary to coat the surface prior to their cultivation.
What is the shelf life of Eppendorf Cell Imaging Consumables?
All Eppendorf Cell Imaging Consumables have a shelf life of 5 years after date of production.
How long do Eppendorf Cell Imaging products stay sterile in the closed packaging?
As long as you keep the packaging closed the products are sterile. Eppendorf guarantees a sterility of 5 years for unopened packages.
Do the adhesives that are used for the Eppendorf Cell Imaging Consumables have any influence on the cells?
The adhesives used for Eppendorf Cell Imaging Consumables are non-cytotoxic and biocompatible according to DIN EN ISO 10993-5:2009.
Which Eppendorf Cell Imaging Consumables are suitable for live cell imaging?
All Eppendorf Imaging Consumables with cover glass and film bottom are best suited for studying living cells: Cell Imaging Dishes, Cell Imaging Plates and Cell Imaging Coverglasses.
Which Eppendorf Imaging Consumable would you recommend for hypoxia-studies?
Eppendorf Imaging Plates with clear film bottom are best suited for cultivating cells under hypoxic conditions or oxygen-controlled conditions, respectively. The 25 µm fluorocarbon film bottom allows optimal gas transfer between cell culture medium and the surrounding Incubator atmosphere.
Can any of the Eppendorf Cell Imaging Consumables be reused?
We do not recommend reusing any of the Eppendorf Cell Imaging Consumables regardless of the format. All Eppendorf Cell Imaging Consumables are intended for single use only.
Which immersion oils are compatible with Eppendorf Cell Imaging products when using oil immersion objectives?
Eppendorf Cell Imaging Consumables are compatible with all standard immersion oils. The use of immersion oil does not lead to damage of the glass or film bottom material.
Is the plastic surface of the dish also tissue-culture (TC)-treated?
Yes, the glass area as well as the whole surface of the Cell Imaging Dish is TC-treated to support attachment of adherent cells.
How do I choose the correct glass bottom thickness?
Eppendorf Cell Imaging Dishes are available with two different cover glass bottom thicknesses: 145 µm (No. 1.0) and 170 µm (No. 1.5). However, for most applications 170 µm is the preferred thickness. Most microscope objectives are designed to be used with a cover glass that has a thickness of 170 µm. The cover glass thickness should correspond to the optimum thickness indicated on the microscope objective.
Do I have to fill the whole Cell Imaging Dish with culture media or is it enough when only the glass bottom is covered with liquid? How are Eppendorf Cell Imaging Dishes typically used?
In general we recommend filling the whole dish with culture media to minimize evaporation and ensure optimal cell growth especially during long-term experiments. The special design of the imaging area as defined central cavity with a depth of 2 mm enables to concentrate cells conveniently in the imaging area and to use very low volumes of e.g. staining substances or antibodies. This helps to reduce the costs of your experiment. The following procedure ensures that the cells grow only within the imaging area: first seed the cells into the central cavity of the dish to restrict cell attachment to the imaging area (cell seeding volume 0.75 mL). After cells have been attached to the glass bottom, the whole dish can be filled with additional media (working volume 2.0 mL).
Can the cover glass bottom be removed from the dish?
No, the cover glass bottom cannot be removed from the dish. The glass is fixed tightly to the dish and there is a risk of injury by breaking the glass when trying to remove it.
Can the chamber of the Eppendorf Cell Imaging Slide and Coverglass be removed without using an additional tool?
Yes, the chambers of both – Slides and Coverglasses - can be removed without an additional tool. However, when working with the Imaging Coverglasses some users might find it more convenient to use e.g. tweezers or a pipette tip to fix the glass bottom when removing the chamber.
Are the chambers / lids made of polystyrene?
No, the chambers are made of COC (cyclic olefin copolymer), which is in contrast to polystyrene resistant to substances like acetone and is more stable to different temperatures.
Is the attachment of the chamber to the Slide / Coverglass leak-tight?
Yes, Eppendorf guarantees the liquid-tightness of all Cell Imaging Consumables. As part of our Quality Control procedure each LOT of products is tested and certified as being leak-tight.
Can the chamber be reused once it is detached from the Slide / Coverglass?
When the chamber is detached it cannot be fixed tightly to a microscope slide or a cover glass again. Apart from this we do not recommend re-using the chamber for any cell culture applications to avoid any risk of contamination because of non-sterile conditions.
What material is used for the Eppendorf Cell Imaging Plates with film bottom plates?
The film bottom of the Eppendorf Cell Imaging Plates is made of fluorocarbon, a material that provides very low autofluorescence and high gas transfer rates.
Can I use the Eppendorf Cell Imaging Plates for automated systems?
The Eppendorf 96-Well Cell Imaging Plates conform to the SBS format. Therefore they fit to most automated systems.
Can the Eppendorf Cell Imaging Plates be used in a centrifuge?
Yes, the Cell Imaging Plates can be centrifuged at a speed up to 300 x g. However, the centrifugation stability of each plate type generally depends on the centrifuge and its accessories, the ambient conditions and the liquid used.
What is the chemical resistance of Eppendorf Cell Imaging Products?
Please find an overview about the chemical resistance of our Cell Imaging Consumables against the most important chemicals here
What certificates are available for Eppendorf Cell Imaging Consumables?
In the attached table
you can see the available certificates for our Cell Imaging Consumables.
What are the specifications of the Eppendorf Cell Imaging Dishes?
Please find an overview on technical data, volumens and optical properties of the Eppendorf Cell Imaging Dishes in this table
What are the specifications of the Eppendorf Cell Imaging Slides & Eppendorf Cell Imaging Coverglasses?
Please find an overview on technical data, volumes and optical properties of the Eppendorf Cell Imaging Slides & Eppendorf Cell Imaging Coverglasses in the table here
What are the specifications of the Eppendorf Cell Imaging Plates?
Please find an overview on technical data, volumes and optical properties of the Eppendorf Cell Imaging Plates in the table here
How does the shaker agitation rate and orbit affect culture growth?
Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double〖RPM〗_1/〖RPM〗_2 = (r_2^2)/(r_1^2 ), then 〖RPM〗_2 = √(〖RPM〗_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm."
Do Eppendorf shakers come with platforms?
Each Eppendorf shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. 250 mL clamps) and provide the greatest capacity but does not allow for the use of different size clamps or holders. The most popular platforms are Universal Platforms which are designed for use with a combination of various-sized clamps and test tube racks providing more flexability. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately. For applications running below 250 rpm Sticky Tape and/or Sticky Pad are also available in lieu of clamps. (However Sticky Pad and Sticky Tape cannot be used in waterbath shakers).
What is the temperature offset function?
The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.
I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?
The Eppendorf Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The Eppendorf Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine."