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Can the Centrifuge 5424/ 5424 R be operated in the cooling room at 4° C?
Can the Centrifuge 5424/ 5424 R be operated in the cooling room at 4° C?
The Centrifuge 5424 can be operated at an ambient temperature in the range from 2° C to 40° C.
Operation in the cooling room at a temperature of down to 4° C is therefore possible. The permitted range of the ambient temperature is between 10 °C and 40 °C for the Centrifuge 5424 R, so that this centrifuge is not to be operated in a cooling room at 4 °C.
Does the lid latch have to be serviced or greased at regular intervals?
Does the lid latch have to be serviced or greased at regular intervals?
No, in order to ensure the full functionality of the lid latch, it must not be cleaned or greased. If you feel that this is nevertheless necessary, please contact our Technical Service. The contact address can be found in the operating manual of your centrifuge.
What is "Fast Temp" (5424R)?
What is "Fast Temp" (5424R)?
The "Fast Temp" function is a temperature control run of the Centrifuge 5424 R without samples und with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and g force (rcf) or speed (rpm) as well as “FT” as symbol for a “Fast Temp” temperature run. You can find a more detailed description in the operating instructions of the Centrifuge 5424/ 5424 R.
When does the continuous cooling of the rotor chamber start (5424R)?
When does the continuous cooling of the rotor chamber start (5424R)?
The continuous cooling starts with each rotor standstill of the Centrifuge 5424 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:
- The centrifuge is switched on.
- The centrifuge lid is closed.
- The nominal temperature is lower than the ambient temperature.
- The centrifuge is not in standby mode.
Continuous cooling is ended by opening the centrifuge lid or by pressing any key.
What is the lowest temperature that can be reached with the Centrifuge 5424 R?
What is the lowest temperature that can be reached with the Centrifuge 5424 R?
The lowest temperature of the Centrifuge 5424 R is -10 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.
What is the temperature range of the Centrifuge 5424 R?
What is the temperature range of the Centrifuge 5424 R?
With the Centrifuge 5424 R it is possible to set the temperature within a range from -10 °C to +40°C.
Does the Centrifuge 5424 R has an energy saving function?
Does the Centrifuge 5424 R has an energy saving function?
Yes, the ECO shut-off automatically switchs off the compressor after 8 hours of non-use.
Can the Centrifuge 5430/ 5430 R be opened in case of a power failure?
Can the Centrifuge 5430/ 5430 R be opened in case of a power failure?
Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic caps on the left-hand and right-hand side of the Centrifuge 5430 have to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.
How can the Centrifuge 5430/ 5430 R be cleaned?
How can the Centrifuge 5430/ 5430 R be cleaned?
The Centrifuge 5430/5430 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.
Sometimes my Centrifuge 5430/ 5430 R displays "EP". What does that mean?
Sometimes my Centrifuge 5430/ 5430 R displays "EP". What does that mean?
"EP" is displayed when the automatic standby function is active. It is not an error code. If the centrifuge remains unused for more than 20 min., the standby function is activated, indicated by "EP" on the display. By using the standby key, the centrifuge is re-activated and ready to use. The letters "EP" were chosen because a lot of Eppendorf products already have this prefix in their name.
Does the Centrifuge 5430/ 5430 R recognize an inserted rotor automatically?
Does the Centrifuge 5430/ 5430 R recognize an inserted rotor automatically?
The Centrifuge 5430/ 5430 R has got an automatic rotor recognition, so that a newly inserted rotor is recognized automatically as soon as a run is started. The maximum speed will be automatically limited for each rotor. This prevents overspeeding of the rotor.
Which material is the standard fixed-angle rotor for micro test tubes made of?
Which material is the standard fixed-angle rotor for micro test tubes made of?
The aerosol-tight standard fixed-angle rotor is made of massive, anodized aluminum. This material is durable and has got a very good chemical resistance due to the special coating. The aerosol-tight rotor lid is made of the same material.
What tubes can be centrifuged with the Centrifuge 5430/ 5430 R?
What tubes can be centrifuged with the Centrifuge 5430/ 5430 R?
Due to the different rotors and adapters, a wide variety of tubes and plates can be centrifuged with the Centrifuge 5430/ 5430 R:
• Micro test tubes (0.5 - 2.0 mL)
• PCR strips and 0.2 mL PCR tubes
• Spin columns
• Cryo tubes
• Conical tubes (15 mL and 50 mL)
• Blood collection tubes
• Oak Ridge Tubes (10 mL, 30 mL and 50 mL)
• PCR plates (96 and 384 well)
• Micro test plates
• Deepwell plates (up to 29 mm height)
• Slides
Can the Centrifuge 5430/5430 R be operated in the cooling room at 4° C?
Can the Centrifuge 5430/5430 R be operated in the cooling room at 4° C?
The Centrifuge 5430 can be operated at an ambient temperature in the range from 4° C to 40° C. Operation in the cooling room at a temperature of down to 4° C is therefore possible. The permitted range of the ambient temperature is between 10 °C and 40 °C for the Centrifuge 5430 R, so that this centrifuge may not be operated in a cooling room at 4 °C.
What tubes do you recommend for centrifugation at 30,000 x g?
What tubes do you recommend for centrifugation at 30,000 x g?
The specifications of the tube manufacturers on the centrifugation resistance must generally be taken into consideration for the centrifugation of tubes of all kinds. This applies in particular for centrifugation at 30,000 x g, as only very few micro test tubes can withstand these extreme gravitational forces to date without breaking. The following tubes can, for example, be centrifuged at 30,000 x g:
• Eppendorf Safe-Lock Tubes 0.5 mL
• Eppendorf Safe-Lock Tubes 1.5 mL
• Eppendorf Tubes 3810X 1.5 mL
When does the opening of the centrifuge lid take place automatically or manually?
When does the opening of the centrifuge lid take place automatically or manually?
Following the end of a centrifuge run, only the lid of the Centrifuge 5430 is automatically opened in order to provide quicker access to the sample and to avoid potential heat accumulation. When working with radioactive material, this function should be deactivated in the device menu and the centrifuge lid manually opened upon conclusion of the centrifugation run. The Centrifuge 5430 R does not come equipped with the option of automatic or manual opening of the centrifuge lid. In order to maintain a consistent temperature in the rotor chamber and in the sample, the centrifuge lid for this device must always be manually opened by pressing the "open" key.
What is "Fast Temp"?
What is "Fast Temp"?
The "Fast Temp" function is a temperature control run of the Centrifuge 5430 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. In addition to the current temperature and the g-count (rcf) / rotational speed, the remaining duration of the temperature control run also appears in the display, so that one can more precisely plan the subsequent workflow. You can find a more detailed description in the operating instructions of the Centrifuge 5430/5430 R.
What can I use the "Fast Temp pro" function for?
What can I use the "Fast Temp pro" function for?
With the "Fast Temp pro" function it is possible to automatically start the "Fast Temp" temperature control run at a predetermined time. In order that the Centrifuge 5430 R already shows the desired temperature at the start of work, one can set a point in time in the device menu at which the temperature control run should be started. The rotor then begins to spin in the centrifuge at a rotor and temperature-specific rotational speed in order to quickly reach the set nominal temperature. This function can thereby be programmed in such a way that it is carried out once or several times. You can find a more detailed description in the operating instructions of the Centrifuge 5430/5430 R.
When does the continuous cooling of the rotor chamber start?
When does the continuous cooling of the rotor chamber start?
The continuous cooling starts with each rotor standstill of the Centrifuge 5430 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:
- The centrifuge is switched on.
- The centrifuge lid is closed.
- The nominal temperature is lower than the ambient temperature.
- The centrifuge is not in standby mode.
Continuous cooling is ended by opening the centrifuge lid or by pressing the standby key.
What is the lowest temperature that can be reached with the Centrifuge 5430 R?
What is the lowest temperature that can be reached with the Centrifuge 5430 R?
The lowest temperature of the Centrifuge 5430 R is -11 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.
What is the temperature range of the Centrifuge 5430 R?
What is the temperature range of the Centrifuge 5430 R?
With the Centrifuge 5430 R it is possible to set the temperature within a range from -11 °C to +40 °C.
Does the Centrifuge 5430 R has an energy saving function?
Does the Centrifuge 5430 R has an energy saving function?
Yes, the ECO shut-off automatically switchs off the compressor after 8 hours of non-use. The device has a stand-by button.
For inactivating prions, can the rotors of the Centrifuge 5430/ 5430R be autoclaved at 135…
For inactivating prions, can the rotors of the Centrifuge 5430/ 5430R be autoclaved at 135 °C ?
The standard rotor FA-45-30-11 can be autoclaved at 135 °C.
Is it possible to operate the Centrifuge 5702 in the cooling room?
Is it possible to operate the Centrifuge 5702 in the cooling room?
Yes, the Centrifuge 5702 may be operated in the cooling room. When moving the centrifuge from the cooling room to a normal lab room, the centrifuge must either run warm for half an hour in the cooling room or warm up for at least 3 hours in the lab room without being connected to the power supply. This is to avoid damage caused by condensation.
Is it possible to operate the Centrifuge 5702 R in the cooling room?
Is it possible to operate the Centrifuge 5702 R in the cooling room?
No, the Centrifuge 5702 R must not be operated in the cooling room. To avoid damage caused by condensation, the centrifuge should be operated in a normal lab room. To ensure optimum operation of the centrifuge, the ambient temperature should be between 10 °C and 40 °C. At temperatures below 18 °C, a warming-up time of approx. 1 hr. is required to ensure faultless operation.
Which material is the rotor chamber made of?
Which material is the rotor chamber made of?
The rotor chamber is made of high-quality steel and is therefore extremely resistant.
How can the centrifuge be opened in the case of power failure?
How can the centrifuge be opened in the case of power failure?
First, pull the plug and wait until the rotor comes to a standstill (can take up to 5 minutes!). The emergency lid release is located on the bottom of the device: a small, white plastic button is located in the base plate in front of the front right device foot. Remove this and pull the cord vertically downwards. The lid will open. Please ensure that the cord retracts completely into the housing when you later close the lid. Press the plastic button back into the base plate.
What is the minimum selectable speed and in which increments can it be increased?
What is the minimum selectable speed and in which increments can it be increased?
The minimum selectable speed is 100 rpm. It can be increased in increments of 100. The g-forces displayed are only approximations. The exact values can be calculated for the different adapters. Please bear in mind that a setting of 100 rpm equals a relative centrifugal force of 1.5 x g. The 100 x g displayed is only reached at a rotational speed of 800 rpm.
Is it possible that the time is counted down only after reaching the selected speed? …
Is it possible that the time is counted down only after reaching the selected speed?
Yes, this is possible. Pressing the START/STOP key for longer than 2 s with the centrifuge lid open initiates the switch to the "at set rpm" mode, as symbolized by a pictogram. To exit the "at set rpm" mode again, press the START/STOP key again for longer than 2 s with the centrifuge lid open, until the pictogram appears.
Is it possible to switch off the signal tone?
Is it possible to switch off the signal tone?
Yes. If the OPEN key is pressed for longer than 2 s while the centrifuge lid is open, either “b on“ (signal tone on) or “b of“ (signal tone off) will appear. With the Centrifuges 5702 R / 5702 RH, a pictogram on the display indicates that the signal tone is switched on.