Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Refrigerated benchtop Centrifuge 5910 R in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. For faster high-yield PBMC isolation, acceleration and deceleration ramps can be selected individually.