Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll® gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Optimized acceleration and deceleration ramps speed up high-yield PBMC isolation up to 88% (see Application Note 372). Refrigerated benchtop Centrifuge 5910 Ri in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. The new user management including run records optimally supports GxP documentation.