Product Solutions for the CAR T Cell Research Workflow

CAR-T Cell Research and Development

Well thought-out tools for pharmaceutical and biotech laboratories

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Product Solutions for the CAR-T Cell Research Workflow

CAR-T cells, genetically engineered and equipped with specific receptors to target and combat cancer cells, are primarily divided into two categories: autologous and allogenic CAR-T cells. Hereby, autologous CAR-T cells derive from the patient’s own white blood cells and are reintroduced after modification, whereas allogenic CAR-T cells are designed as off-the-shelf solutions for use in different patients. However, both approaches have their challenges. The manufacturing process for autologous CAR-T cells is complex and costly, as each patient’s cells must be individually processed. Allogeneic CAR-T cells, on the other hand, currently do not have the same safety profile, necessitating further research to address these issues.

ADVANCING CAR-T CELL RESEARCH: FROM PBMC ISOLATION TO LARGE-SCALE PRODUCTION

Reproducable PBMC isolation

The Centrifuge 5910 Ri has soft acceleration/break ramps for optimized PBMC isolation and significant time savings during Ficoll gradient separation. High throughput with up to 4 × 1,000 mL working volume ideal for cell harvesting on the benchtop.

Contamination proof thawing

The Eppendorf ThermoMixer^® C and the SmartBlock cryo-thaw exchangeable block offers a special thawing program that supports reproducible, reliable thawing of frozen PBMCs up to 1 mL in standard 1.8-2 mL cryovials.

Safe and consistent culturing of cells

Ensure optimal activation of T-Cells with the uniform atmosphere of the CellXpert^® C170i. Fanless design, seamless chamber, 180 °C sterilization cycle, and a removable water tray for reliable contamination control.

Safer pipetting

The ep Dualfilter T.I.P.S.^® pipette tips are proven to provide the optimized protection of your pipette and sample against unintentional and unnoticed contamination during viral work.

Best conditions for your mammalian suspension cell culture!

The CellXpert CS220 ensures precise temperature and CO₂ control, minimized evaporation by active humidity control and reliable shaking. The stainless steel
chamber and integrated 180 °C sterilization protect your cells from contaminations.

Optimized cell expansion

The DASbox^® Mini Parallel Bioreactor System provides precise control over culture conditions in a small volume range (100 mL-250 mL), optimizing CAR-T cell growth and viability during the crucial expansion phase.

Reliable and traceable sample storage

Freeze down your CAR-T cells and digitally store data (donor, date, CAR contstruct etc.) with the Safe Code system to readily access sample information even after transfer to LN2 (compatible with any barcode scanner).

Speed up your characterization assays

The Eppendorf Xplorer^® electronic pipettes enables quick filling of plates using the dispensing mode. When connected to the Pipette Manager, an even quicker operation of the pipette is possible.

Simplify your scale-up

With the BioFlo^® 320, scaling production is simpler than ever. Benefit from our intuitive Scale-Up Assist software, ensuring easy transition from bench to production while maintaining optimal growth conditions and process efficiency.

^{*All products are for Research Use Only (RUO).}