I have noticed that more solution is used for reverse pipetting than for normal pipetting. What is the reason for this?

Reverse pipetting means uptake of liquid including the blow-out volume and dispensing of liquid without the blow-out volume. Reverse pipetting is recommended to reduce the error with viscous or foaming liquids or when dispensing very small sample volumes. The excess solution aspirated remains in the tip and is discarded.

Can you recommend any literature on reverse pipetting?

Userguides 20 and 21 describe the technique and give application examples. They can be downloaded from the Knowledge Base on our website.

Should the pipette tip be wetted with liquid before pipetting volumes smaller than 10 µL?

Regardless of the volume, the air cushion should be wetted by multiple filling and emptying of the tip before dispensing.

What is the best way to pipette viscous liquids?

When pipetting viscous liquids, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).

Is there an Eppendorf pipette which can be used to pipette 40 % polyethylene glycol 8000 (PEG)?

Yes, for pipetting PEG instruments operating according to the positive displacement principle like the Biomaster or a Multipette/Repeater can be used.

What is the best way to pipette chloroform?

When pipetting chloroform, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).

What is the best way to pipette diethyl ether?

Diethyl ether has such a high vapor pressure that it would leak out of the tip immediately when an air cushion pipette is used. For this reason, it can only be pipetted using a positive displacement pipette, such as Biomaster, Multipette/Repeater, and Varipette.

Are Eppendorf pipettes resistant to acetonitrile?

Yes, Eppendorf pipettes are resistant to acetonitrile but care must be taken to avoid damage to printing and display window.

What should be considered when pipetting methanol with air cushion pipettes?

Due to the increased vapor pressure, we recommend that the tip is prewetted at least 3 times and that the reverse pipetting technique is used.

What is the best pipette for pipetting a 99% solution of n-amyl alcohol?

A 99 % solution of n-amyl alcohol is a highly viscous solution. We recommend working with an instrument that operates according to the positive displacement principle like Multipette/Repeater, Varipette, or Biomaster.

What is the best way to dispense organic solvent?

It is best to use a positive displacement device like the Multipette/Repeater. Positive displacement devices are most suitable for precise and drip-free dispensing of liquids with high vapor pressure. As the liquid is enclosed in a tip with integrated piston, the solvent vapor does not come into direct contact with the instrument.

I would like to pipette dichloromethane and n-hexanol very precisely. Can I use air cushion pipettes for this application?

Both dichloromethane and n-hexanol have a very high vapor pressure. For that reason, positive displacement instruments like the Biomaster and the Multipette/Repeater should be used.

Are your pipettes free of nucleic acids?

No, we cannot guarantee this. Of course we work carefully and cleanly in production, but no special measures are taken to prevent the infiltration of nucleic acids. To protect your pipette from contamination, we recommend using positive displacement pipettes, for example, our Biomaster or filtertips (ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax).

How can radioactive contamination be removed from Eppendorf pipettes?

Following contamination of pipettes with radioactive liquids, we recommend placing the contaminated parts in a complex-forming liquid or special cleaning solution. Then rinse very thoroughly with distilled water and dry. Grease piston lightly afterwards.

Do Eppendorf pipettes always need to be greased?

No, the piston only has to be greased if grease-dissolving solutions like organic solvents have been used.

Can the pipette withstand evacuation (applying a vacuum)?

No, air cushion pipettes should not be used under vacuum, as the pressure ratios are not right.

How should warm or cold liquids be pipetted?

Exactly in the same way as liquids at room temperature, however, please note that solutions may behave differently. In addition, the pipetting accuracy / precision may be outside the permissible error limits. In order to achieve maximum accuracy / precision, all components should have the same temperature: pipette, tips, liquid, and the surrounding air.

Are the handles of Eppendorf pipettes made of a special material to minimize temperature effects during pipetting?

Temperature effects are minimized in our pipettes by their design. We use a special polypropylene which satisfies the special requirements on a pipette handle.

Do Eppendorf pipettes always have to be kept in a stand or can they also be put down on the laboratory bench?

They can be put down on the laboratory bench - but never with a full tip.

I often work with different chemicals. Can you tell me when I need to adjust my pipettes?

This belongs to the permissible errors of your specific application. In general an adjustment of air cushion pipettes, in other words altering the stroke of the pipette, is required for solutions of a density higher 1.2 mg/µL. Please be aware that variable manual pipettes turn into "fixed-volume pipettes" when being adjusted: They can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. Eppendorf manual pipettes have a user adjustment for temporary adjustment purposes. This user adjustment can easily be set back to manufacturer settings without the need for recalibration. For the correct procedure and examples for settings of user adjustments for different liquids please see the operating instructions and CD enclosed to your pipette.

How do I determine the volume values when calibrating gravimetrically?

The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.

What does EN ISO 8655 cover?

ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters: ISO 8655-1: Terminology, general requirements, and user recommendations ISO 8655-2: Piston pipettes ISO 8655-3: Piston burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric method for the determination of measurement error ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)

What do the terms random error and systematic error mean in a pipette calibration?

The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

At which volumes should a variable pipette be calibrated?

According to the EN ISO 8655 standard, a variable pipette should be calibrated to 3 volumes during conformity testing: 1. Nominal volume, 2. 50 % of nominal volume, 3. Smallest value that can be set or 10 % of nominal volume.

What is the nominal volume of a variable pipette?

The nominal volume of a variable pipette is always the maximum volume. For a 100 - 1,000 µL pipette, it is therefore 1,000 µL.

I very frequently work with different chemicals. Can I still perform pipette calibration using water?

Calibration, i.e. checking a pipette on a balance, is always performed using distilled or deionized water (see EN ISO 8655).

During weighing (pipette calibration) I obtained steadily decreasing measuring results. Why is this?

The weight fluctuations you measured would appear to be a temperature problem. Please ensure that the pipette, the distilled water used for testing, the tips etc. are all at the same temperature. It is recommended that the pipettes be placed in the weighing room at least 2 hours before the test is performed. While measuring, ensure that transfer of heat from your hands is kept to a minimum (otherwise weight fluctuations may occur!) Tip: do not constantly grasp the pipette in the palm of your hand while waiting for each measurement result! We recommend our standard operating procedure (SOP) for pipettes as a source of basic information on pipette calibration.

How are multi-channel pipettes tested?

According to ISO 8655, all channels of multi-channel pipettes are to be tested. All channels have to be filled but only the channel to be tested is measured. Because of the high number of measurements (8-channel pipette: 240 measurements, 12-channel pipette: 360 measurements), it makes sense to send the multi-channel pipette to a professional calibration lab. These labs have special constructions for balances to ensure that all channels are filled but only one channel is measured.

Is the pipette grease free of resins?

Yes, the pipette grease supplied by Eppendorf is free of resins.

How is the wall mount for pipettes attached to the wall?

The pipette holder is affixed to the wall with adhesive.

How many times can Reference 2 be autoclaved?

Our pipettes are checked for a minimum lifetime of five years. This means a normal use with 200,000 strokes, 26,000 volume changes, 100,000 tip ejections, and 175 autoclavings without the pipetting results and pipette material being affected.

Do I have to regrease the Reference 2 after autoclaving?

If the pipette grease is not removed actively (e.g., by cleaning with alcohols before autoclaving), there is no need to regrease the piston-cylinder system. During endurance testing (200,000 strokes, 26,000 volume changes, 100,000 tip ejections, and 175 autoclavings), the Reference 2 was not regreased and gravimetrically checked after each autoclaving. No decline in systematic and random measurement error was observed. If regreasing is needed, Eppendorf grease for Research plus and Reference 2 must be used. Only this grease withstands the heat in the required manner.

The housing of Reference 2 contains different materials. What effects does this have on the pipette during autoclaving?

The Reference 2 is mounted in a way that all materials have room for expansion. Furthermore, only materials are used which are resistant to severe conditions, e.g. autoclaving (pressure, rise in temperature).

Is it possible to inactivate the spring-loaded tip cone of a Reference 2 single-channel pipette?

Yes. The scope of delivery includes a locking ring with which the spring-loaded tip cone can easily be inactivated. Disassemble the upper from the lower part of the pipette and push the locking ring onto the lower part from the top. Please refer to the operating manual for an illustrated description.

Is it possible to inactivate the spring-loaded tip cone of a Reference 2 multi-channel pipette?

Yes. The Reference 2 multi-channel pipettes have a switch on the lower part which inactivates/activates the spring-loaded tip cone.

Where and how can the Reference 2 pipettes be adjusted?

Reference 2 pipettes offer factory adjustment and user adjustment. The factory adjustment should be changed if the pipette was found to be out of specifications during calibration. Before adjusting, exclude other causes such as pipette handling, environmental errors, or worn-out parts. The user adjustment is an easy way of adjusting the pipette temporarily to different conditions (e.g. pipetting a liquid with high density). Please note that the adjustment is only valid for the chosen volume and not for the full volume range.

How often should pipettes be calibrated?

The user decides upon the frequency of calibration, the number of test volumes and the number of measurements for each test volume within the framework of monitoring his test materials. This can vary depending on the frequency of use of the pipette, the number of users of the device, the aggressiveness of the liquids to be dispensed and the error limits deemed acceptable by the user. However, an inspection should be carried out at least once a year. See also EN ISO 8655-6.

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Eppendorf Reference^® 2 - Single-Button Pipette

Eppendorf Reference® 2 single-channel pipette and multi-channel pipette

Eppendorf Reference® 2 four-digit volume display for easy, quick and accurate volume setting

Eppendorf Reference® 2 single-channel pipette along with 8- and 12-channel choices

Changing volume settings on the precise Eppendorf Reference® 2 pipette is quick and easy

Eppendorf Reference® 2 mechanical pipette in use with a tube

On/Off switch to deactivate spring-loaded tip cones on Eppendorf Reference 2 multi-channel lower part

Eppendorf Reference® 2 single-channel pipette family

Eppendorf Reference® 2 multi-channel pipette family

View of parts for the Eppendorf Reference® 2 mechanical pipette

epT.I.P.S.® macro volume tips facilitate easy reach to the bottom of e.g. 15 mL centrifugation tubes or flasks

The Pipette Carousel 2 with space for up to six Eppendorf pipettes

Laboratory technician using an Eppendorf Reference® 2 mechanical single-button pipette in the lab

Laboratory technician with Eppendorf Reference® 2 mechanical pipette in the lab

Laboratory technician adjusting Eppendorf Reference® 2 one-button pipette in the lab

Product Information

Choose the Eppendorf Reference 2 mechanical pipette with ergonomic single-button operation and benefit from its high precision and accuracy with reduced operating efforts. Its smooth surface and full autoclavability guarantee efficient decontamination - making it the ideal companion when working under sterile conditions. Available as a single-channel pipette in fixed or variable volume, and as 8- and 12-channel pipettes - all with time-saving single-button operation. Once you have experienced this unique operating philosophy you will not want to change back!

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Product Information

Technical Data

Applications

Features

A premium pipette that delivers extraordinary precision and accuracy

The Eppendorf Reference 2 mechanical air-cushion pipette with outstanding precision and accuracy is extremely reliable and robust, allowing for a long service life. With its unique single-button operation, the Reference 2 provides fast and ergonomic handling with reduced operating efforts and active aerosol reduction. The clear haptic feedback helps to avoid accidental tip ejection. Don’t compromise on quality and reproducibility – choose the Eppendorf Reference 2 to confidently pipette precious samples accurately and improve your results.
The sturdy handle manufactured with stainless steel protects the pipette from impact and in combination with spring-loaded bumpers, the Reference 2 pipette is likely to stay within calibration even if it takes a fall. These high levels of security make the Reference 2 your ideal companion in applications requiring extremely high levels of accuracy.

Fast and ergonomic single-button operation

With the precise Eppendorf Reference 2 pipette, you can benefit from reduced strain thanks to lower operating forces for aspiration, dispensing and tip ejection. Users can also experience faster operation times as there is no need to move your thumb from side to side to eject tips, meaning you can move quickly through pipetting taks. And as tips are ejected immediately following dispensing, you can benefit from a reduced aerosol contamination of the pipette.

User-friendly temporary adjustment option

It is important to make sure you adjust your pipette according to sample type. The Reference 2 mechanical pipette allows you to do this easily without disturbing factory settings. A temporary and simple adjustment allows for an easy alteration of your Reference 2 to perform accurate pipetting of different liquids. Recommended settings are provided in the manual and you can easily and quickly reset to the manufacturer settings.

The ideal lab companion in sterile work environments

The Reference 2 pipette can easily be taken apart, is fully autoclavable and features a smooth housing being made in one pour. This offers advantages for any researcher concerned about bacterial contamination. With its low-adhesive surface and a minimum of cavities, grooves and sliding parts, the Reference 2 is easy to decontaminate and particularly suitable for use in class A/B cleanrooms and other sterile environments.

You have the choice: we offer a variety of single- and multi-channel options

Not all experiments are based on the same working conditions. Depending on the vessel type, you can choose from single-channel Reference 2 pipettes that are ideal for working with tubes and flasks, and multi-channel Reference 2 pipettes better suited for plates.

• Tubes & flasks: Single-channel choices, available as variable or fixed-volume pipettes
• 96- and 24-well plates: 8- and 12-channel pipette choices with option to deactivate the spring-loaded tip cones.

Fatigue-free and ergonomic pipetting

With it's unique single-button operation, the Eppendorf Reference 2 pipette features an innovative and highly ergonomic operation concept that was designed in line with the Eppendorf PhysioCare Concept ^® to minimize hand and arm strain. As most other Eppendorf pipettes, the Reference 2 premium pipette is of course equipped with the spring-loaded tip cone and SoftEject technologies from Eppendorf.

Spring-loaded tip cones allow for reliable tip fit and effortless attachment

Eppendorf pipettes with spring-loaded tip cone save you time and energy. With haptic feedback, all it takes is one gentle push-down for easy tip attachment. The spring limits the attachment forces and at the same time guarantees a reproducible and safe tip fit. This clever design provides increased user-to-user reproducibility and more consistent results between different lab members. All micropipettes from Eppendorf up to 1,000 µL (except Move It^® 300 µL models) are equipped with the spring-loaded tip cone feature. In all Reference 2 multi-channel pipettes, the springs can easily be deactivated with an "On/Off" switch on the pipette lower part.

SoftEject technology reduces tip ejection forces by 50 %

All non-adjustable multi-channel pipettes from Eppendorf use the innovative SoftEject technology to ensure that during each single tip ejection stroke, the outside tips eject before the inside tips. This is not noticeable to the user but reduces tip ejection forces by 50 %. You just lightly press the ejection button once and the tips are automatically ejected in succession.

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Specifications	Eppendorf Reference^® 2 - 4924000010	Eppendorf Reference^® 2 - 4924000029	Eppendorf Reference^® 2 - 4926000018	Eppendorf Reference^® 2 - 4926000026	Eppendorf Reference^® 2 - 4924000037	Eppendorf Reference^® 2 - 4924000045	Eppendorf Reference^® 2 - 4924000053	Eppendorf Reference^® 2 - 4926000034	Eppendorf Reference^® 2 - 4926000042	Eppendorf Reference^® 2 - 4924000061	Eppendorf Reference^® 2 - 4924000070	Eppendorf Reference^® 2 - 4926000050	Eppendorf Reference^® 2 - 4926000069	Eppendorf Reference^® 2 - 4924000088	Eppendorf Reference^® 2 - 4924000096	Eppendorf Reference^® 2 - 4924000100	Eppendorf Reference^® 2 - 4924000118	Eppendorf Reference^® 2 - 4925000014	Eppendorf Reference^® 2 - 4925000022	Eppendorf Reference^® 2 - 4925000030	Eppendorf Reference^® 2 - 4925000049	Eppendorf Reference^® 2 - 4925000057	Eppendorf Reference^® 2 - 4925000065	Eppendorf Reference^® 2 - 4925000073	Eppendorf Reference^® 2 - 4925000081	Eppendorf Reference^® 2 - 4925000090	Eppendorf Reference^® 2 - 4925000103	Eppendorf Reference^® 2 - 4925000111	Eppendorf Reference^® 2 - 4925000120	Eppendorf Reference^® 2 - 4925000138	Eppendorf Reference^® 2 - 4925000146	Eppendorf Reference^® 2 - 4925000154	Eppendorf Reference^® 2 - 4925000162	Eppendorf Reference^® 2 - 4925000170

Product type	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes	Pipettes
Pipetting type	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system	Air-cushion system
Number of channels	1-channel	1-channel	8-channel	12-channel	1-channel	1-channel	1-channel	8-channel	12-channel	1-channel	1-channel	8-channel	12-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel	1-channel
Volume range	0.1 – 2.5 µL	0.5 – 10 µL	0.5 – 10 µL	0.5 – 10 µL	2 – 20 µL	2 – 20 µL	10 – 100 µL	10 – 100 µL	10 – 100 µL	20 – 200 µL	30 – 300 µL	30 – 300 µL	30 – 300 µL	100 – 1,000 µL	0.25 – 2.5 mL	0.5 – 5 mL	1 – 10 mL	1 µL	2 µL	5 µL	10 µL	10 µL	20 µL	20 µL	25 µL	50 µL	100 µL	200 µL	200 µL	250 µL	500 µL	1,000 µL	2 mL	2.5 mL
Color code	dark gray	medium gray	medium gray	medium gray	light gray	yellow	yellow	yellow	yellow	yellow	orange	orange	orange	blue	red	violet	turquoise	dark gray	dark gray	medium gray	medium gray	yellow	light gray	yellow	yellow	yellow	yellow	yellow	blue	blue	blue	blue	red	red
Volume increment	0.002 µL	0.01 µL	0.01 µL	0.01 µL	0.02 µL	0.02 µL	0.1 µL	0.1 µL	0.1 µL	0.2 µL	0.2 µL	0.2 µL	0.2 µL	1 µL	0.002 mL	0.005 mL	0.01 mL	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−	−
Operating mode	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical	mechanical
Spring-loaded tip cone	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	no	no	no	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	no	no
Random measurement error	0.1 µL: ± 12%; ± 0.012 µL 0.25 µL: ± 6%; ± 0.015 µL 1.25 µL: ± 1.5%; ± 0.01875 µL 2.5 µL: ± 0.7%; ± 0.0175 µL	0.5 µL: ± 5%; ± 0.025 µL 1 µL: ± 1.8%; ± 0.018 µL 5 µL: ± 0.8%; ± 0.04 µL 10 µL: ± 0.4%; ± 0.04 µL	0.5 µL: ± 8%; ± 0.04 µL 1 µL: ± 5%; ± 0.05 µL 5 µL: ± 2%; ± 0.1 µL 10 µL: ± 1%; ± 0.1 µL	0.5 µL: ± 8%; ± 0.04 µL 1 µL: ± 5%; ± 0.05 µL 5 µL: ± 2%; ± 0.1 µL 10 µL: ± 1%; ± 0.1 µL	2 µL: ± 1.5%; ± 0.03 µL 20 µL: ± 0.6%; ± 0.06 µL 20 µL: ± 0.3%; ± 0.06 µL	2 µL: ± 1.5%; ± 0.03 µL 20 µL: ± 0.6%; ± 0.06 µL 20 µL: ± 0.3%; ± 0.06 µL	10 µL: ± 0.7%; ± 0.07 µL 50 µL: ± 0.3%; ± 0.15 µL 100 µL: ± 0.2%; ± 0.2 µL	10 µL: ± 2%; ± 0.2 µL 50 µL: ± 0.8%; ± 0.4 µL 100 µL: ± 0.3%; ± 0.3 µL	10 µL: ± 2%; ± 0.2 µL 50 µL: ± 0.8%; ± 0.4 µL 100 µL: ± 0.3%; ± 0.3 µL	20 µL: ± 0.7%; ± 0.14 µL 100 µL: ± 0.3%; ± 0.03 µL 200 µL: ± 0.2%; ± 0.4 µL	30 µL: ± 0.7%; ± 0.21 µL 150 µL: ± 0.3%; ± 0.45 µL 300 µL: ± 0.2%; ± 0.6 µL	30 µL: ± 1%; ± 0.3 µL 150 µL: ± 0.5%; ± 0.75 µL 300 µL: ± 0.3%; ± 0.9 µL	30 µL: ± 1%; ± 0.3 µL 150 µL: ± 0.5%; ± 0.75 µL 300 µL: ± 0.3%; ± 0.9 µL	100 µL: ± 0.6%; ± 0.6 µL 500 µL: ± 0.2%; ± 1 µL 1.000 µL: ± 0.2%; ± 2 µL	250 µL: ± 1.2%; ± 3 µL 500 µL: ± 0.3%; ± 1.5 µL 1250 µL: ± 0.2%; ± 2.5 µL 2500 µL: ± 0.2%; ± 5 µL	500 µL: ± 0.6%; ± 3 µL 2500 µL: ± 0.25%; ± 6.25 µL 5.000 µL: ± 0.15%; ± 7.5 µL	1.000 µL: ± 0.6%; ± 6 µL 5.000 µL: ± 0.2%; ± 10 µL 10.000 µL: ± 0.15%; ± 15 µL	1 µL: ± 1.8%; ± 0.018 µL	2 µL: ± 1.2%; ± 0.024 µL	5 µL: ± 0.6%; ± 0.03 µL	10 µL: ± 0.5%; ± 0.05 µL	10 µL: ± 0.6%; ± 0.06 µL	20 µL: ± 0.3%; ± 0.06 µL	20 µL: ± 0.3%; ± 0.06 µL	25 µL: ± 0.3%; ± 0.075 µL	50 µL: ± 0.3%; ± 0.15 µL	100 µL: ± 0.2%; ± 0.2 µL	200 µL: ± 0.2%; ± 0.4 µL	200 µL: ± 0.2%; ± 0.4 µL	250 µL: ± 0.2%; ± 0.5 µL	500 µL: ± 0.2%; ± 1 µL	1.000 µL: ± 0.2%; ± 2 µL	2.000 µL: ± 0.2%; ± 4 µL	2.500 µL: ± 0.2%; ± 5 µL
Systematic measurement error	0.1 µL: ± 48%; ± 0.048 µL 0.25 µL: ± 12%; ± 0.03 µL 1.25 µL: ± 2.5%; ± 0.031 µL 2.5 µL: ± 1.4%; ± 0.035 µL	0.5 µL: ± 8%; ± 0.04 µL 1 µL: ± 2.5%; ± 0.025 µL 5 µL: ± 1.5%; ± 0.075 µL 10 µL: ± 1%; ± 0.1 µL	0.5 µL: ± 12%; ± 0.06 µL 1 µL: ± 8%; ± 0.08 µL 5 µL: ± 4%; ± 0.2 µL 10 µL: ± 2%; ± 0.2 µL	0.5 µL: ± 12%; ± 0.06 µL 1 µL: ± 8%; ± 0.08 µL 5 µL: ± 4%; ± 0.2 µL 10 µL: ± 2%; ± 0.2 µL	2 µL: ± 3%; ± 0.06 µL 20 µL: ± 1%; ± 0.1 µL 20 µL: ± 0.8%; ± 0.16 µL	2 µL: ± 5%; ± 0.1 µL 20 µL: ± 1.2%; ± 0.12 µL 20 µL: ± 1%; ± 0.2 µL	10 µL: ± 3%; ± 0.3 µL 50 µL: ± 1%; ± 0.5 µL 100 µL: ± 0.8%; ± 0.8 µL	10 µL: ± 3%; ± 0.3 µL 50 µL: ± 1%; ± 0.5 µL 100 µL: ± 0.8%; ± 0.8 µL	10 µL: ± 3%; ± 0.3 µL 50 µL: ± 1%; ± 0.5 µL 100 µL: ± 0.8%; ± 0.8 µL	20 µL: ± 2.5%; ± 0.5 µL 100 µL: ± 1%; ± 1 µL 200 µL: ± 0.6%; ± 1.2 µL	30 µL: ± 2.5%; ± 0.75 µL 150 µL: ± 1%; ± 1.5 µL 300 µL: ± 0.6%; ± 1.8 µL	30 µL: ± 3%; ± 0.9 µL 150 µL: ± 1%; ± 1.5 µL 300 µL: ± 0.6%; ± 1.8 µL	30 µL: ± 3%; ± 0.9 µL 150 µL: ± 1%; ± 1.5 µL 300 µL: ± 0.6%; ± 1.8 µL	100 µL: ± 3%; ± 3 µL 500 µL: ± 1%; ± 5 µL 1.000 µL: ± 0.6%; ± 6 µL	250 µL: ± 4.8%; ± 1.2 µL 500 µL: ± 1.5%; ± 7.5 µL 1250 µL: ± 0.8%; ± 10 µL 2500 µL: ± 0.6%; ± 15 µL	500 µL: ± 2.4%; ± 12 µL 2500 µL: ± 1.2%; ± 30 µL 5.000 µL: ± 0.6%; ± 30 µL	1.000 µL: ± 3%; ± 30 µL 5.000 µL: ± 0.8%; ± 40 µL 10.000 µL: ± 0.6%; ± 60 µL	1 µL: ± 2.5%; ± 0.025 µL	2 µL: ± 2%; ± 0.04 µL	5 µL: ± 1.2%; ± 0.06 µL	10 µL: ± 1%; ± 0.1 µL	10 µL: ± 1.2%; ± 0.12 µL	20 µL: ± 0.8%; ± 0.16 µL	20 µL: ± 1%; ± 0.2 µL	25 µL: ± 1%; ± 0.25 µL	50 µL: ± 0.7%; ± 0.35 µL	100 µL: ± 0.6%; ± 0.6 µL	200 µL: ± 0.6%; ± 1.2 µL	200 µL: ± 0.6%; ± 1.2 µL	250 µL: ± 0.6%; ± 1.5 µL	500 µL: ± 0.6%; ± 3 µL	1.000 µL: ± 0.6%; ± 6 µL	2.000 µL: ± 0.6%; ± 12 µL	2.500 µL: ± 0.6%; ± 15 µL
Autoclavable	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
Consumables	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®	epT.I.P.S.^®
Open system (third party consumables)	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes	yes
Delivery content	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, O-ring tool	Pipette, epT.I.P.S.^® Box 2.0 pipette tips, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, epT.I.P.S.^® pipette tips sample bag, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Filter sleeve, Filter cartridges (x10), Pipette wrench	Pipette, epT.I.P.S.^® pipette tips sample bag, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Filter sleeve, Filter cartridges (x10), Pipette wrench	Pipette, epT.I.P.S.^® pipette tips sample bag, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Filter sleeve, Filter cartridges (x10), Pipette wrench	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)	Pipette, Operating manual, Certificate, Adjustment seal for temporary adjustment, Adjustment seal for factory adjustment (x2), Grease for pipettes, Locking ring (to prevent spring action)

Downloads: Eppendorf Reference^® 2

Flyer

Eppendorf Pipette Multi-Packs (Starter Kits)

Pick your pack and save on Eppendorf pipettes with our 3- and 6-packs

PDF .7MB

English

Selection Guide

Get It Right! Selection Chart for Liquid Handling Instruments & Tips

Find the most suitable pipetting system for your lab applications

PDF 1.5MB

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Flyer

Increase Your Pipetting Productivity Today

Higher throughput and increased reproducibility with Eppendorf multi-channel pipettes

You give your all to scientific research every day. Eppendorf liquid handling instruments help you grow beyond your limits

PDF 6.2MB

English

Brochure

Pipette Service Done Right

Eppendorf Pipette Calibration

PDF 3.0MB

English

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Eppendorf Reference® 2 - Single-Button Pipette

Eppendorf Reference® 2 single- and multi-channel pipettes with one-button operation

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Episode 1: Micropipettes

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Product Information

Eppendorf Reference® 2 One-Button Pipette

Products (34)

Sets & Bundles (8)

Services (36)

Accessories (16)

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Product Information

A premium pipette that delivers extraordinary precision and accuracy

Fast and ergonomic single-button operation

User-friendly temporary adjustment option

The ideal lab companion in sterile work environments

You have the choice: we offer a variety of single- and multi-channel options

Fatigue-free and ergonomic pipetting

Spring-loaded tip cones allow for reliable tip fit and effortless attachment

SoftEject technology reduces tip ejection forces by 50 %

Technical Data

Applications

Features

Downloads: Eppendorf Reference® 2

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