Multiple kits for whole genome library preparation exist on the market. For an exemplary comparison of library preparation time, steps and costs of different kits, see table 1. With the release of the Nextera® DNA Flex Library Preparation kit, Illumina® introduced a new chemistry which streamlines these time-consuming steps into a faster, highly flexible workflow. To even further improve the library preparation, automated liquid handling systems should be used. Ideally, minimal hands-on time is necessary and the system is optimized to work with the kit. This saves preparation time and guarantees reproducibility of library preparations. Two things play important roles: Logically compartmented workflows and testing thorough or qualifying the kit method.