Search Results for: „pipettes”

Yes, Eppendorf pipettes are resistant to acetonitrile but care must be taken to avoid damage to printing and display window.

Eppendorf tubes, plates including LoBind, and pipette tips are resistant to 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

​All Eppendorf bio-based products fulfill the same standards as fossil-based Eppendorf consumables: They perform the same mechanical and chemical stability and have the same low leaching profile. A detailed overview of quality criteria for Eppendorf products regarding raw material and production is provided in APPLICATION NOTE NO. 056.

Yes, for the high-volume pipettes 2.5 mL, 5 mL and 10 mL. Nose cone filters are filters which are pushed into the nose cone.

As the liquid normally does not contact the pipette, the application is possible. If the material of the pipette gets in direct contact for a longer time the tip ejector can be damaged (it can be changed as a spare part).

"Eau de Javel" is the old name for a bleach solution containing potassium hypochlorite (KOCl). Solutions containing NaOCl, which ought to behave very similarly to KOCl in chemical terms, have been tested. These solutions can be used with our tips and for decontaminating the surface of Research pipettes. Commercially-available surface decontaminants like RNase AWAY and DNA AWAY are also possible. If the surface is treated for less than an hour, subsequent cleaning with distilled, sterile water is required. After an extended period (a few months), plastic parts may be affected by the NaOCl.

No.

The upper part of the multi-channel pipette can be used with both 8-channel and 12-channel lower parts, insofar as the volume sizes are taken into account.

Compared to the multi-channel pipette, the single-channel Research pro 20 - 300 µl pipette has slight differences at the slowest (for gel loading) and fastest speeds. However, this has no effect on the "Mix" function.

Yes, the material of the pipette is resistant to toluene.

The customer spare parts for the Research 3 neo pipettes can be found on the product page under 'spare parts' or in the Operating Manual chapter 12.3-12.6.

We cannot guarantee that our pipettes are completely free of nucleic acids. While we maintain high standards of cleanliness and care during production, we do not implement specific measures to prevent nucleic acid contamination. To protect your samples from potential contamination, we recommend using positive displacement devices or filter tips, such as ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax.

According to the EN ISO 8655-2022 standard, three series of 10 measurements are used to calibrate a variable-volume pipette. The three test volumes are:

1. Nominal volume (the largest volume adjustable by the user, as determined by the manufacturer)
2. 50% of the nominal volume
3. 10% of the nominal volume, or the lower limit if the minimum is over 10%

The factory adjustments of the fixed-volume Pipette Research plus cannot be adapted by the users. These adjustments can only be performed by a qualified Eppendorf service technician and a subsequent calibration is necessary.

Yes. Because every channel functions individually, the coincidental measurement deviation (imprecision) of the pipette is not influenced.

Yes. But direct contact of chloroform with the pipette should be avoided. To pipette solutions with high vapour pressures, we recommend the use of pipettes that function according to the positive-displacement technique, e.g. Biomaster or Multipette.

Yes, you can define your own liquid types by selecting the vapor pressure, dynamic viscosity and the desired piston speed.

Yes, the Research 3 neo pipette has a volume lock. The device is delivered in an unlocked state. By moving the slider below the volume display to the closed lock symbol on the left, the volume lock is active and the volume cannot be changed.

It is possible to activate the user management and protect different user accounts with a PIN or password. In addition, the user accounts can be defined with different access levels.

The Pipette Manager offers a user management with different accounts protected with a PIN or password. For each account, you may set a different user role with restricted, standard or administrator rights.

Like all our single-use plastic items, our pipette tips are also made of polypropylene and can therefore be stored at -10°C even for many years.
Please note that you should keep the pipette tips for several minutes at room temperature before pipetting.

If you want to use a connected pipette without the Pipette Manager, you can simply turn the selection dial from Opt to any operating mode you want and use it as any other non-connected pipette.

Yes, the Research 3 neo is designed for use with epT.I.P.S. and ep Dualfilter T.I.P.S. pipette/filter tips in different variants and purity grades.

In the "Pipetting and Mixing" mode, the sample is automatically mixed after dispensing. The mixing cycles and volume can be individually defined.

RFID is currently not supported. Pipettes can be added via QR codes and scanners. Import of existing documentation material is possible.

No. Only bottom parts with the same variable volume are generally interchangeable.

Yes, batteries for electronic liquid handling can be ordered separately as spare parts (4861601000) and should be replaced after approx. 3 years of use.

The ColorTag rings are fully autoclavable (121°C, 1 bar overpressure for 20 minutes) with no observed effect on the material. Slight color changes can occur which have no effect on the functionality of the rings.

No, air cushion pipettes should not be used under vacuum, as the pressure ratios are not right.

No. But the entire pipette can be autoclaved at 121 °C.

The Reference and Research plus pipettes can be autoclaved completely, from the Research pipette the lower part is autoclavable. The precision and accuracy are not affected.

Yes, the pipette offers two adjustment options. The temporary adjustment allows the user to adjust for liquid type, tip geometry, altitude, or reverse pipetting to maintain accuracy without recalibration and quickly change back to the factory setting/respectively last calibration. It can be found on the side of the pipette below the cover labelled 'ADJ'. Please refer to the Operating Manual chapter 6.12 for further instructions regarding the temporary adjustment and our tutorial video.
The factory adjustment changes the adjustment of the pipette permanently and can be found below the volume gear. A check of the factory adjustment is necessary after replacing volume-determining parts and is usually performed by professional service providers.

Yes, the Research 3 neo pipette can be disassembled. There is a detailed description of the disassembly in the Operating Manual chapter 7.1.3 - 7.1.6 as well as a tutorial video available.

The Research 3 neo pipettes are suitable to withstand H2O2 fumigation. 10 fumigation cycles with a H2O2 concentration of minimum 400 ppm and 3h contact time under laboratory conditions have been tested with no observed effect on material or function.

No, the Research pro does not fit in the pipette carousel, only in the charging stand designed for it.

Eppendorf manual pipettes have both, a user adjustment for temporary adjustment purposes and a factory adjustment. This user adjustment can easily be set back to manufacturer settings without the need for recalibration. Please be aware that variable manual pipettes turn into "fixed-volume pipettes" when being adjusted: They can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. For the correct procedure and examples for settings of user adjustments for different liquids please see the operating instructions and CD enclosed to your pipette.

No.
The principle of the Dualfilter (in ep Dualfilter T.I.P.S. and ep Dualfilter T.I.P.S. SealMax and their biobased variants) is to protect from aerosols, meaning small liquid droplets in the air and for SealMax also from liquid splashes. Gaseous vapour/fume from liquid samples is not retained by any filter. To protect the lower part of a pipette, it is best to use a lower part made of PTFE (higher chemical resistance, to withstand highly aggressive chemicals (e.g. TFA)). Please note that Eppendorf offers a PTFE lower part for some single-channel Research plus and Reference 2 pipettes. Important to bear in mind when using it: A lower pipette part made of PTFE is not autoclavable as PTFE is not dimensionally stable under heat. Pipette calibration is recommended after replacing the lower part.

The potential release of trace metal ions from the epTIPS under extreme conditions was measured by an independent and certified laboratory. The certificate that provides exact concentration per metal is provided as a pdf on our certificates homepage .

The pipette can be placed on the laboratory bench, but never with liquid inside the tip. In general, it is best to keep pipettes on the pipette carousel. When storing pipettes in a drawer, ensure the pipette does not come into contact with the drawer walls or other equipment, as this may damage the pipette when the drawer is opened or closed.

No, the ColorTag pipette marking rings are fully autoclavable and does not interfere with the autoclaving of your pipette.

No. The VisioNize^® Lab Suite cloud solution for remote device monitoring and maintenance scheduling is a separate product offering. No subscription plan is needed to work with the Pipette Manager.

No. The Pipette Manager uses the built-in WiFi to establish connections to electronic pipettes upgraded with an Eppendorf Xplorer^® connect module or tablets. It does not connect to the internet.

Eppendorf does not only service and calibrate Eppendorf pipettes, but also other brands of pipettes and dispensers.

You can turn the Pipetting Records mode on and off whenever you want. You can always pause a recording and work without or switch to another recording and continue. Once you finished the steps you want to record, you can finish the recording and export it for documentation.

Yes, after cleaning, the piston should be re-greased. A special grease is supplied to enable you to do this.

No, the Research 3 neo pipettes does not need to be disassembled before autoclaving. However, the protection filter in the 2 mL, 5 mL and 10 mL pipettes must be removed prior to autoclaving. The filter sleeve and the protection filter should be autoclaved separately. The ColorTag marking rings can remain attached.

No, the Pipette Manager is designed for flexible and spontaneous pipetting and rapid adjustments of workflows.

The weight fluctuations you measured would appear to be a temperature problem. Please ensure that the pipette, the distilled water used for testing, the tips etc. are all at the same temperature. It is recommended that the pipettes be placed in the weighing room at least 2 hours before the test is performed. While measuring, ensure that transfer of heat from your hands is kept to a minimum (otherwise weight fluctuations may occur!) Tip: do not constantly grasp the pipette in the palm of your hand while waiting for each measurement result! We recommend our standard operating procedure (SOP) for pipettes as a source of basic information on pipette calibration.

The calibration of connected electronic pipettes can be done with or without the Pipette Manager just like the calibration of any other electronic pipette by a professional pipette service provider like epServices or following the Standard Testing Procedure for Manual Dispensing Systems provided by Eppendorf.

Organic, heavy-metal-free dyes (PP granulate) are used to tint the pipette tips.

According to ISO 8655-2022, every channel of a multi-channel pipette must be tested. While all channels need to be filled, only the channel being tested is measured. Due to the high number of measurements required (e.g., 240 measurements for an 8-channel pipette and 360 measurements for a 12-channel pipette), it is advisable to send the multi-channel pipette to a professional calibration lab. These labs are equipped with specialized balance setups that ensure all channels are filled while only one channel is measured at a time.

Upon manufacturing, pipettes are calibrated to distilled water. Eppendorf mechanical pipettes allow temporary user adjustments, beneficial for liquids with different physical properties than water (density, viscosity, surface tension, vapor pressure), or when capillary actions occur (e.g., with DMSO). Adjustments are also beneficial when atmospheric pressure changes due to altitude or when using non-standard pipette tips (e.g., epT.I.P.S. long). The adjusted pipette delivers the correct volume only for the liquid used during adjustment. Please be aware that variable mechanical pipettes turn into "fixed-volume pipettes" when being adjusted. That means they can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. Research plus and Reference 2 pipettes feature an adjustment window where the change can be monitored. It can be set back to factory settings "0". For detailed procedures and examples, refer to the operating instructions and Application Note No. 198.

In contrast to mechanical pipettes, the electronic Xplorer/Xplorer plus pipettes can be adjusted for the entire range of volume. Additionally, the electronic pipettes offer several options for adjusting the pipette in the "Options" menu, e.g. to 75 % ethanol, altitude, elongated tips. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings. For more information see User Guide 036.

Temporary user adjustments can be reset to manufacturer settings without recalibration.

To clean the housing moisten a cloth with cleaning agent and remove external contamination. Wipe down the housing with water afterwards.
To clean the touch screen, select Menu - Clean Screen. Wipe the glass plate of the touch screen with a cloth and a mild cleaning agent. Touch the corners in the numbered sequence to unlock the touch screen.

You can acquire new features for free by downloading the latest software version from the Eppendorf Download Center and transferring it to the Pipette Manager using an USB drive. The update can be started by selecting Menu, Settings, System Settings, Update Software. The software update does not remove any data or settings from your device.
Software Downloads

In order to ease the tip attachment to the nose cone and to reduce the ejection forces, we recommend to use the new O-rings (red color) immediately.
The new O-rings are maintenance free and made of silicone. The new and the old rings are differentiated by the new red color.
We recommend to exchange the old O-rings. Order no. for multi-channel pipettes 100 / 300 µl: 4908 836.001 (12 pieces)
Order no. for multi-channel pipettes 1200 µl: 4908 716.009 (8 pieces).

Following contamination of pipettes with radioactive liquids, we recommend placing the contaminated parts in a complex-forming liquid or special cleaning solution. Then rinse very thoroughly with distilled water and dry. Grease piston lightly afterwards.

To calibrate Research 3 neo pipetted in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum volume to be dispensed), 50% of the nominal volume and 10% of the nominal volume of the Research 3 neo tips used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in the "Eppendorf SOP for checking pipettes" available for download at the product page.

Simply scan the QR-codes under Menu, Settings, System Settings, Network, Wi-Fi Access with your tablet. Scan Wi-Fi Access with QR Code to access the WiFi of the Pipette Manager. Then scan Access Interface to open the Pipette Manager interface in the tablet's browser.

Simply upgrade your electronic pipette with an Eppendorf Xplorer^® connect module and connect it with the supplied USB cable to the Pipette Manager. The Pipette Manager will notify you if an update is required. Once the update is performed, turn the selection dial to Opt. Select Wi-Fi and Connect AUTO. On the Pipette Manager, select Menu, Pipette Manager, Pair New Pipette and wait for approx. 20 sec.

First of all, the Move It is a normal pipette which, accordingly to ISO 8655, needs to be calibrated at least once a year. You can send the pipettes to your service provider, to the eppendorf service or do the calibration yourself. Maintenance should be performed by service providers only.

Depending on the liquid type, the Pipette Manager will automatically include required pre-wetting steps, define the appropriate pipetting speed or disable unsuitable operating modes. These functions can be customized by defining your own liquid types, which can be shared with your colleagues.

The pipette holder is affixed to the wall with adhesive.

The lithium polymer battery features high energy density and its service life usually spans several years, if properly maintained and stored. It is recommended however, to replace the battery after approx. 3 years of use.

​The Pipette Manager provides 30 open connections which you can use to connect either pipettes or tablets to manage connected pipette sets.
Example set ups for 5 or 6 workspaces:
a) 1 Pipette Manager + 4 tablets each connected to a pipette set of 5 pipettes.
b) 1 Pipette Manager + 5 tablets each connected to a pipette set of 4 pipettes.

The user determines the frequency of calibration. However, according to EN ISO 8655-2022 Part 1, calibration must be performed regularly and at a minimum, once per year. Routine tests should be performed more frequently, ideally at intervals shorter than those for calibration. The frequency of both calibration and routine tests should be established through a risk assessment. This assessment needs to consider factors such as the frequency of pipette use, the number of users, the aggressiveness of the liquids being dispensed, and the acceptable error limits set by the user. Additionally, any industry- or organization-specific frequency requirements must be adhered to.

As part of his test equipment monitoring process, the operator determines the frequency of the calibration as well as the quantity of the testing volume and the number of measurements for each testing volume. This may vary depending on our how often the pipette is used, by how many operators the device is used, how aggressive the liquids are that are to be pipetted and the maximum permissible errors defined by the operator. However, there should be at least 1x inspection per year. Also refer to ISO 8655.

A professional inspection of the Research 3 neo pipette including calibration should be carried out at least once a year, see also EN ISO 8655.
The user decides upon a higher frequency of calibration, the number of test volumes and the number of measurements for each test volume within the framework of monitoring their test materials. This can vary depending on the frequency of use of the pipette, the number of users of the device, the aggressiveness of the liquids to be dispensed and the error limits deemed acceptable by the user.

The calibration of connected electronic pipettes can be done like a calibration of a usual electronic pipette by using a calibration service. No additional efford is needed. Liquid types can not be calibrated.

The ColorTag marking rings can be cleaned with various cleaning agents, e.g. 70% ethanol. Please refer to the chemical resistance chapter in the the ColorTag Instructions for Use (IFU). It is recommended to clean ColorTag rings and pipettes separately, to allow the cleaning liquid to reach the surface below the ring. The ColorTag rings can be autoclaved either attached or separately from the pipette. It is also possible to sterilize them with UV-C irradiation or H2O2 fumigation.

Please refer to the Operating Manual chapter 7.2 for detailed instructions on how to clean, decontaminate and disinfect the pipette. A detailed list of suitable cleaning agents is provided as well as instructions on how to autoclave, treat the pipette with UV-light and apply H2O2 fumigation.

The Research 3 neo pipette should be checked for any damage on the outside before use. If the pipette is damaged, it must not be used and has to be sent in for service.
The Operating Manual chapter 7 provides detailed instructions on how to maintain the pipette.
Moreover, a professional inspection including calibration should be carried out at least once a year, see also EN ISO 8655.

All Eppendorf Xplorer^® and Xplorer plus pipettes with an on-off push button (instead of a switch) can be updated. This includes all Eppendorf Xplorer/plus pipettes manufactured since 2015. The corresponding Eppendorf Xplorer software version for the pipettes with a on-off push button is 03.01.00 or later. In order to find out the software version of your pipette, please check via: Options / Service / Softwareversion, to check if your Xplorer/ plus pipette can be connected to VisionNize pipette manager.

Dismantle the pipette into its individual parts (to do this, see operating instructions) and check whether there is any contamination. If so, you can wipe this off with a cloth and reassemble the pipette. Then recalibrate your pipette and follow the instructions in our SOP as you do so. Values which deviate significantly from the limit value should now no longer occur. If there is no contamination, please send the pipette to our pipette service department.

As per EN ISO 8655-2022, pipette calibration, which involves checking liquid dispensed by the pipette on a balance, should always be performed using distilled or deionized water. If you are working with chemicals that have different properties than water, consider using the temporary adjustment to minimize errors as outlined in Application Note 198. In general, we recommend using direct displacement devices such as the Repeater M4/E3(x) for challenging liquids.

You can use the shelf stand (4430603006) that is provided in the pack and available as an accessory. By attaching it to the back of the Easypet 3, you can park the Easypet 3 without contaminating the attached pipette.

Both dichloromethane and n-hexanol have a very high vapor pressure. For that reason, positive displacement instruments like the Biomaster and the Multipette/Repeater should be used.

No, provided that you work with Eppendorf single-channel pipettes and your sample is an aqueous solution. In this case, you can recover nearly 100% of your aqueous sample. Simply press down the operating button of your pipette slowly and dispense the liquid. The recovery rate of water has been shown in Application Note 273.

In this case the lowest possible volumes are 50 µl for samples and 450 µl for diluent. The largest volumes are 100 µl for sample and 900 µl for diluent.

Yes: The Pipette Holder 2 (white) is designed to hold Research 3 neo pipettes. The holder can be attached to the wall with adhesive tape or used with the Eppendorf Pipette Holder System (stand and carousel).

No, this is not necessary.

Yes, Move It^® Xplorer variants are compatible with the VisioNize^® pipette manager and can be connected.

Yes. The Reference 2 multi-channel pipettes have a switch on the lower part which deactivates/activates the spring-loaded tip cone.

Yes, microtiter plates can be filled using the 8-channel Research pipette. The spacing between nose cones is 9.1 mm, corresponding to the dimension between holes from well to well of MTP and PCR plates.

Yes. The Reference 2 multi-channel pipettes have a switch on the lower part which inactivates/activates the spring-loaded tip cone.

Yes, the delivery includes a locking ring to easily deactivate the spring-loaded tip cone. Disassemble the upper from the lower part of the pipette and push the locking ring onto the lower part from the top. Refer to the Operating Manual for an illustrated guide. This is useful for pipette tips not designed for Eppendorf pipette cones.

No, currently the Pipette Manager software is only available in English.

No, currently the software of the VisioNize^® pipette manager is only available in English.

The complete lower part of Pipette is autoclavable. Pull off / unscrew the ejection sleeve and disassemble the lower part using the tool provided. The pipette can also be decontaminated using UV light.

Yes, the pipette grease supplied by Eppendorf is free of resins.

Yes, the piston is made of teflon, the cylinders are made of PP. These materials are resistant.

Yes. However, the material may change color when exposed to intense UV light or when exposed to normal UV light for a prolonged period. This discoloration has no effect on the function, precision and accuracy of the pipette.

Yes, the Research 3 neo pipette can be autoclaved at 121°C and 1 bar overpressure for 20 minutes. The pipette should be cleaned, set to unlock and the protection filter and filter sleeve of the 2, 5 and 10 mL pipettes should be removed and autoclaved separately. No further disassembly is required.
Cool the pipette to ambient temperature and let it dry. Greasing is not necessary after autoclaving. For maximum precision and accuracy, a gravimetric test is recommended after autoclaving.

The Research 3 neo pipettes are resistant to UV-C irradiation. Pipettes might show some discoloration, which has no effect on functionality or pipetting performance (accuracy and precision). We recommend the use of an Hg-lamp, 254 nm, 30 W. The distance between lamp and pipettes should be approx. 60 cm. A dosage of up to 1200 J/cm² UV-C (254 nm) has been successfully tested.

Yes. However, the material may change color when exposed to intense UV light or when exposed to normal UV light for a prolonged period. This discoloration has no effect on the function, precision and accuracy of the pipette.

No. This applies to both the single- and multi-channel pipette.

There is no difference between connecting Eppendorf Xplorer or Xplorer plus pipettes. All features of the Pipette Manager are available on both models – Xplorer pipettes even gain additional pipetting modes (Sequential Dispensing, Sequential Pipetting, Diluting, Reverse Pipetting, Multi Aspiration).
The Programming mode as well as the History and Adjustment features can be exclusively used with Xplorer plus pipettes in offline mode though.

Yes, for pipetting PEG instruments operating according to the positive displacement principle like the Biomaster or a Multipette/Repeater can be used.

Please refer to our ColorTag Pipette Marking Rings – Selection Guide or the ColorTag Instructions for Use (IFU) for information on the available colors and sizes of ColorTag marking rings and their compatibility to Eppendorf manual Liquid Handling products.

The Research pro pipette is stable to UV. In the event of prolonged irradiation with UV light, material discolorations may occur, but these do not affect the proper function of the pipette.