Are you working with cells?
Are you working with cells?

Share with us your experience and tell us what you would expect from Eppendorf. Please take part in our short survey.

Cell Biology

Eukaryotic Cells

Cell Biology is a branch of biology that studies cells - the organelles they contain, their function, their physiological properties, their life cycle, the interactions with their environment etc. Cell Biology basic research can be divided into several subfields e.g. the study of cell metabolism, the study of cell genetics and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell communication and signaling. Research in cell biology overlaps to a great extent with other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology.

Cell manipulation

Cell manipulation

The TransferMan® 4 and InjectMan® 4 have been developed for precise micromanipulation of adherent and suspension cells. Application specific user profiles simplify the individual workflow process and the DualSpeed joystick combines direct and dynamic movement control.

 

 

  • Micromanipulators
    • Maximum stability ensures vibration-free work
    • One-hand operation for capillary and angle settings
    • Programmable Z-axis limit for preventing capillary breakage
    • Connection with Eppendorf PiezoXpert® and electronic microinjectors
    • Can be adapted to all conventional microscopes
  • Microinjectors
    • Very simple and clearly arranged operation for routine tasks
    • Injection volume ranges from femtoliters to microliters
    • Inject and Clean function can be triggered right on the device using the included mouse or optional foot control
    • Programmable injection guarantees high reproducibility
    • Connection to TransferMan® 4r, InjectMan® 4 or InjectMan®NI 2 enables semi-automatic microinjection
  • Capillaries
    • VacuTips for holding of suspension cells
    • TransferTips for stem cell transfer etc.
    • Femtotips and Femtotip II for microinjection of minimal volumina
    • Piezo-Drill Tips for piezo-assisted micromanipulation
  • Photometers
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetic
      (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM to 2,000 nM fluorescein with the BioSpectrometer fluorescence
  • Centrifuges
    • Max. rotor capacity: 48 x 1.5/2.0 mL, 6 x 50 mL, 2 x MTP
    • Max. speed: 30,130 x g (17,500 rpm)
    • Remarkable versatility with 12 different rotors
    • Soft-touch one-finger lid closure for ergonomic operation
    • Menu-driven, multi lingual operation menu (English, German,
      French, Spanish) with large backlit display
Cell seeding

Cell seeding

The Easypet 3 in combination with the serological pipettes enables you to control the forces that act on your cells during pipetting. Besides this, Eppendorf offers a new generation of Cell Culture Consumables with improved handling and stacking performance, optimized cell accessibility and safety.

  • Pipette controllers
    • Intuitive and convenient speed adjustment made simple with the tips
      of your fingers
    • Lightweight, well-balanced and ergonomic design that allows for fatigue-free pipetting
    • Vibrant backlit LEDs provide optical feedback of the remaining battery life
    • Lithium polymer rechargeable battery offers long cordless runtime
    • Smooth setting of pump speed
  • Serological Pipettes
    • Individually wrapped
    • Easy to open paper/plastic wrap
    • Subunits with dispenser option to keep stored products safe
    • Harmonized and well-fitting operation with Easypet 3, compatibility
      with all existing pipet helper
    • Sharp and clear graduation for facilitated read-out
  • Automation
    • 4 ANSI/SBS worktable and 3 virtual positions
    • Maximum pipetting accuracy from 1 to 1,000 μL
    • Calibrated 1-channel and 8-channel pipetting tools
    • Optical sensor1 for detecting liquids, labware, tips
    • Compatible with tubes (0.2 mL to 50 mL) and microplates
      with up to 384 wells
  • Dishes, Flasks & Plates
    • Unsurpassed safety in handling during transportation and while working due to corrugated handling ring
    • SplashProtect ring inside of the dish lid traps liquid and prevents spills during transportation or incubation
    • Robust stacking performance by pronounced rims on dish lid and exact fitting of lid and bottom when used in stacks
    • Easy differentiation of lid and dish bottom prevents unintended removal of the lid
    • Packaging combines tool-free opening, resealable top and side for tape-free closing and an easy solution to shrink the package for space-conscious storage
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
Cell cultivation

Cell cultivation

Our Cell Culture Consumables are developed to facilitate the lab work while offering safety and convenience in handling. With our comprehensive range of bioprocess solutions cells can be cultivated under controlled conditions, e.g. using single-use vessels.

 

 

  • Bioreactors
    • Rigid wall stirred-tank single-use vessels covering working volumes from 100 mL - 40L
    • Wide range of industry standard autoclavable glass bioreactors
    • Stainless steel sterilize-in-place vessels
  • Bioprocess Systems
    • Comprehensive and scalable hardware and software solutions for R&D, process development, pilot, and production
    • Small scale and scale down models, parallel operated bioreactors, bench scale solutions, and sterilize-in-place systems
    • Covering working volumes of 60mL - 520L
  • Dishes, Flasks & Plates
    • Easy and fast well identification by contrast rich individual well ID and OptiTrack® alphanumeric labeling
    • Moat surrounding the outer wells to prevent the “edge effect” when filled with liquid
    • Chimney-well design to enable the filling of the inter well spaces of the complete plate to level out well-to-well temperature shifts outside the incubator
    • Robust stacking performance by pronounced rims on plate lid and excellent fitting of lid and base when used in stacks
    • Lid taps under the plate for minimized surface contact and therefore reduced risk of contamination
  • CO2 Incubators
    • Temperature range from 4 °C above ambient to 50 °C
    • Shaking speeds between 25 – 400 rpm (±1 % full scale), 25 – 300 rpm if stacked, provides versatility for culturing a wide range of cell types.
    • Large 35.6 x 61 cm (14 in x 24 in) platform accommodates flasks up to 4 liters providing flexability with your cultures
    • Single or double stacked for incresed capacity
    • Inner and outer door saves valuable gasses while maintaining the perfect enviornment for your cells
  • Pipettes and Tips
    • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid an accidential tip ejection a clear haptic feedback is provided.
    • The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
    • The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
    • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids or other external conditions without the need for a full calibration.
    • The quick lock enables an easy cleaning and maintenance of your pipette.
Harvesting & Preparation

Harvesting & Preparation

The speed adjustment of our electronic pipettes helps to remove the cells very carefully and precise. A variety of different consumables such as the well-known Safe-Lock Tubes or our Conical Tubes (15 mL and 50 mL) can be used for the preparation and/ or sample collection.

  • Centrifuges
    • Max. speed: 3,000 x g (4,400 rpm)
    • Very compact footprint to fit on every lab bench
    • Very quiet operation to benefit your environment
    • Low access height for simple loading and unloading of samples
    • SOFT brake function for slow acceleration and braking. Optimized for cell separation by gradient centrifugation
  • Biopur® consumables
    • Safety guaranteed, even for the most sophisticated applications
    • Reduced costs - inconclusive, usually expensive tests no longer necessary
    • "Ready for use" products reduce labor and time required
    • Individually blistered consumables and “micro batches” in re-sealable boxes provide effective protection against soiling or contamination
  • Tubes & plates
    • Hinged Eppendorf Safe-Lock lid prevents unintentional lid opening during incubation and storage
    • Polypropylene provides high resistance to chemicals, mechanical stress and temperature extremes
    • Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio-assay results
    • g-Safe®: Exceptional centrifugation stability up to 30,000 x g prevents sample loss due to tube breakage and provides extra safety when working with hazardous samples
    • Precise lid sealing for minimal evaporation rates during long-term storage
  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity.
Analysis & Detection

Analysis & Detection

With the Eppendorf Detection equipment molecular analysis such as nucleic acid and protein quantification, kinetic studies, ELISA, fluorescence or microvolume measurements can be performed.

  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
  • Reader Plates
    • UV-VIS microplates feature an ultrathin film bottom for excellent light transmission in the UV range
    • Black Microplates offer an excellent signal-to-noise ratio - for clear signals even with low-concentration samples
    • White Eppendorf Microplates are optimized for highest sensitivity
      in the detection of luminescence signals by maximizing reflection
    • Solid black and white assay plates are made of polypropylene resulting
      in high resistance to chemicals, mechanical stress and temperature extremes
    • All plates are optimized for minimal autofluorescence and autoluminescence
  • Thermocyclers
    • Intuitive graphic programming
    • Small footprint
    • Universal block for ultimate flexibility
    • Connect up to 2 Mastercycler nexus eco to your existing unit
    • Available with gradient
  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
  • Cell Imaging consumables
    • Robust plate base with excellent planarity
    • TC treated surface for improved cell adhesion
    • Low skirt for full access to outer wells
    • 24-well and 96-well formats with glass or film bottom for high flexibility
Storage

Storage

The New Brunswick freezers meet the highest standards for quality in regard to performance, sample security, and convenience. To concentrate your samples prior to the storage, the Concentrator plus is available as well as different sizes and qualities of tubes and plates.

  • Freezer
    • Provides 30% more storage capacity than freezers of equal size
    • Inner doors in upright models are gasketed and insulated creating three separate compartments minimizing cold air loss
    • Unique heated air vent with plunger to clear ice buildup and to relieve vacuum formation to gain immediate access to the freezer
    • Built-in voltage stabilizer on U535, U725, C585 and C760 freezers provides extra sample security
  • Deepwell plates
    • OptiTrack® matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with
      heat sealing
    • Easy and reliable stacking
  • Conical Tube
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high.
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity.
     
    • Sealing Options
      • Storage Film and Foil glued seals for simple and dependable sealing
        during sample storage
      • Heat Sealing Film and foil for continuous locking with the best
        evaporation protection (see heat sealing materials)
      • Foil products are made of aluminum, can be pierced, and protect
        light-sensitive samples
      • Film products are transparent and provide protection against
        unwanted punctures
      • Eppendorf Plate Lid: stable, flexible protection of samples during
        short-term storage
      • Concentrator
        • Comes in 3 system configurations
        • Extremely quite operation <50 dB(A)
        • Chemical-resistant, maintenance-free PTFE diaphragm pump eliminates the need for changing up pump oil
        • Small footprint saves valuable bench space
        • Wide variety of 15 different rotor options to accommodate tubes
          from 0.2 mL to 50 mL, microplates and PCR plates