Webinars

Troubleshooting in bioprocessing

Successful bioprocessing is complex and depends on many factors. From the selection of the expression host, media composition, the proper cultivation system and cultivation strategy, adequate downstream processes, up to the development of a robust tech transfer.                                                                              

In this webinar we will address general and day-to-day challenges in the bioprocessing workflow from shake flask to production and discuss possible solutions. We will initially look on common challenges in reactor set-ups and configurations. What vessel and impeller type are the appropriate one for different culture demands (e.g. shear sensitivity, high oxygen demands), what process parameters control is essential and how the integration of additional analytical tools can help to improve process stability. We will address frequent challenges in cultivation, e.g. how to produce reproducible inoculums in shake flasks, how to prevent contamination in a bioreactor, during transfer or sampling and how to cope with difficulties in cultivation in the bioreactor, e.g. foaming or viscous solutions. We will further have a look on the different steps of a process and discuss optimization factors and measures.

Register now, if you want to have answers to frequently asked questions in bioprocessing and want to understand how to cope with challenges in bioprocessing from shake flask to bioreactor.     

If you want to get answers on specific questions during the webinar, send an email to bioprocess-experts@eppendorf.com by June 6.

Key Learning Objectives:

  • Get answers to frequently asked questions in bioprocessing.
  • Learn how to define and optimize important parameters to improve culture results.
  • Get hands-on advice to optimize each step of your process.
  • Live “Ask the Expert” session – Deepen your knowledge and optimize your process.

Duration (optional): 1 hour

Date & Time: Wednesday, June 23, 2021 at 9am EST| 2pm BST| 3pm CEST

Speakers:

  •  Ines Hartmann, Application Specialist Cell Handling, Eppendorf AG
  •  Ankita Desai, Bioprocess Field Application Specialist, Eppendorf North America

Register here

Ines Hartmann, Application Specialist Cell Handling, Eppendorf AG
Ankita Desai, Bioprocess Field Application Specialist, Eppendorf North America

Cell line identity- Preventing cell misidentification in your daily lab routine

1951 the first human cell line HeLa entered cell culture labs worldwide. 70 years later, we know that at least 129 cell lines are contaminated with HeLa cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lines are misidentified (ICLAC 2020) reveals a significant need for solid cell authentication that is increasingly required by scientific journals and auditing authorities.

In this webinar, we will discuss principles, methods, and share best practices to check cell identity. Join us to learn more about preventing cell misidentification by incorporating Good Cell Culture Practice into your daily routine and become a true cell culture expert.

Do you have questions for the experts?

Join the live Q&A session to deepen your knowledge, ask questions, and optimize your process. All questions asked during the live webinar or recorded session will be answered by our experts – during the Q&A session or afterwards.

Scheduling conflict?
If you can´t make it, just register to receive the link to the recorded session.

In this webinar you will learn:

  • Cell misidentification - What's that?
  • Explain and learn about causes and consequences of working with the wrong cell line
  • Discuss how to choose a cell authentication method
  • Discuss the background of STR amplification and capillary electrophoresis (CE) for successful cell authentication and how to apply these methods correctly

Date & Time: June 9, 2021 at 7am PDT, 10am EDT, 4pm CEST

Speakers:

  • Jessica Wagener, PhD, Application Specialist Cell Handling, Eppendorf AG
  • Gabriela Saldanha, Senior Product Manager, Promega Corp.

Register here

Jessica Wagener, PhD, Application Specialist Cell Handling, Eppendorf AG
Gabriela Saldanha, Senior Product Manager, Promega Corp.