The Art of Correct Pipetting

The use of pipettes is a part of scientists’ lab routines around the world. Pipetting techniques are generally passed down from seasoned colleagues to newcomers. But differences in pipetting techniques can also have a significant impact on the replicability of scientific findings. For this reason, an expert introduction to the correct handling and use of pipettes for various pipetting methods should be a fundamental aspect of every lab training program. But “old hands in the lab” can also profit demonstrably from guidance about optimal pipetting techniques, the essence of daily lab work. After all, even the smallest application failures can significantly impact the accuracy and replicability of findings.

But flawless pipetting techniques are not the only key factor in lab work. The right tools as well as the quality and frequency of their maintenance and calibration play a critical role as well. This is a lesson Dr. Nicole Kühl, the owner of InCelligence, an independent life-science training portal, has also learned. Driven by the desire to get to the bottom of things, to have an impact and to help people, the scientist established the information and training platform InCelligence in 2012. Together with her team of cell and molecular biologists, BTAs, MTAs and pharmacists, she has been tirelessly working since then to compile a base of knowledge about laboratory methods and tools, cell culture, quality management (GMP), PCR/RT-PCR, sequencing, NGS, protein analysis, ELISA and much more as well as to share this knowledge on her information platform and in special training courses. This work also includes independent comparisons of such products as pipettes , pipette tips , incubators , microscopes and cell-culture consumables. InCelligence rounds out its profile by offering comprehensive assistance with troubleshooting and standardization through SOPs as well as consulting and advisory services on the establishment of bioassays and testing in a regulated environment.