Benefits of Dispensers in Cell Culture

Lab Academy 14. Juni 2018

In cell culture avoiding contamination is of paramount importance. Positive displacement dispensers can have advantages over air-cushion pipettes and pipet controllers both in terms of preventing contaminations and reproducibility of results.

Benefits of positive displacement dispensers in cell culture are manifold. A major advantage is complete aerosol prevention as the sample is hermetically sealed in the tip. This ensures that no contamination of the instrument or cross-contamination between different samples will occur. In addition, keeping the cells in suspension by inverting the Combitip is very easy. And dispensing them into the target vessel more quickly can lead to better reproducibility.

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Reproducibility for cell seeding varies with the pipetting system used. The figure shows viable cell signal relative to number of seeded cells for air-cushion pipette (yellow), positive-displacement system (blue) and pipet controller (red).

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Eppendorf solutions

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Benefits of Dispensers in Cell Culture

Eppendorf solutions