Incorporating cell authentication testing into cell culture routine
Whereas no mandatory testing schedule exists at this time, it is recommended to systematically check and document cell line identity at least:
- When a foreign cell line enters the cell culture laboratory.
- During establishment of frozen cell stocks.
- At the beginning and at the end of an experimental project.
- Before distributing a cell line.
Choosing a cell authentication method
Several different methods are available to detect inter-species and intra-species cross-contamination in cell cultures. These methods verify cell line identity on the protein or genetic level. The acquisition and application of authentication methods is not difficult for trained scientists. However, reliable interpretation of the results requires advanced experience. Depending on the testing method, special laboratory equipment might also be necessary. For support, commercial services may be employed to perform testing and/or interpretation of analysis within a short turnaround time.